CASK Antibody (YA6492)
(Synonyms: CAGH39 antibody; Caki antibody; Calcium/calmodulin dependent serine protein kinase antibody; Calcium/calmodulin dependent serine protein kinase (MAGUK family) antibody; Calcium/calmodulin dependent serine protein kinase membrane associated guanylate kinase antibody; Calcium/calmodulin-dependent serine protein kinase antibody; CAMGUK antibody; CAMGUK protein antibody; CAMGUK, drosophila, homolog of antibody; casK antibody; CAGH39 antibody; Caki antibody; Calcium/calmodulin dependent serine protein kinase antibody; Calcium/calmodulin dependent serine protein kinase (MAGUK family) antibody; Calcium/calmodulin dependent serine protein kinase membrane associated guanylate kinase antibody; Calcium/calmodulin-dependent serine protein kinase antibody; CAMGUK antibody; CAMGUK protein antibody; CAMGUK, drosophila, homolog of antibody; casK antibody; CMG antibody; CSKP_HUMAN antibody; DXPri1 antibody; DXRib1 antibody; FGS4 antibody; FLJ22219 antibody; FLJ31914 antibody; hCASK antibody; LIN 2 antibody; Lin 2 homolog antibody; LIN2 antibody; Lin2 homolog antibody; MICPCH antibody; MRXSNA antibody; Pals3 antibody; Peripheral plasma membrane protein CASK antibody; Protein lin-2 homolog antibody; TNRC8 antibody; Trinucleotide repeat containing 8 antibody; Vertebtate LIN2 homolog antibody; )CASK Antibody (YA6492) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CASK.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:2000 | 1:500-1:2000 |
Product Details
CASK Antibody (YA6492) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CASK.
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Host Mouse
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 105 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 105 kDa
Recombinant protein within Human CASK aa 311-513 / 926.
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Membrane-associated guanylate kinase CASK is a multidomain MAGUK protein with Ca²⁺/calmodulin-kinase, PDZ, SH3, and guanylate kinase domains, and it localizes to neuronal synapses[1]. Mechanistically, CASK scaffolds synaptic protein complexes through interactions with neurexin, Veli/LIN-7, Mint 1, Caskin 1, syndecan-2, and PMCA4b[1][2][3][4]. CASK also links membrane signaling to nuclear regulation, because PMCA4b binding reduced T-element-dependent transcriptional activity in a Ca²⁺-dependent reporter system[4]. In disease models, CASK deletion in mice caused neonatal lethality and altered spontaneous glutamatergic and GABAergic release, while missense mutations associated with neurodevelopmental disorders disrupted binding to SAP97, CINAP, Tbr1, and neurexin[5][6]. Compared with related MAGUK proteins, CASK is distinguished by its Ca²⁺/calmodulin-dependent kinase domain and by alternative transcript variants that change binding affinities to interaction partners[5][7]. For experimental design, current literature supports CASK mutation, knockdown, knockout, splicing, and protein-interaction assays, but it does not establish validated CASK agonists or inhibitors for pathway-specific intervention[5][6][7].
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Subcellular Localization
Nucleus,Cytoplasm,Cell membrane
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Expression
Tissue_Specificity: Ubiquitous. Expression is significantly greater in brain relative to kidney, lung, and liver and in fetal brain and kidney relative to lung and liver -
Isoforms & Post-Translational Modification
O14936 has 6 isomers: O14936-1: 105123 Da (predicted); O14936-2: 104520 Da (predicted); O14936-3: 102114 Da (predicted); O14936-4: 102165 Da (predicted); O14936-5: 59383 Da (predicted); O14936-6: 103211 Da (predicted).
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Subunit
CASK and LIN7 form two mutually exclusive tripartite complexes with APBA1 or CASKIN1 (By similarity)
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SwissProt ID
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Synonyms
CAGH39 antibody; Caki antibody; Calcium/calmodulin dependent serine protein kinase antibody; Calcium/calmodulin dependent serine protein kinase (MAGUK family) antibody; Calcium/calmodulin dependent serine protein kinase membrane associated guanylate kinase antibody; Calcium/calmodulin-dependent serine protein kinase antibody; CAMGUK antibody; CAMGUK protein antibody; CAMGUK, drosophila, homolog of antibody; casK antibody; CAGH39 antibody; Caki antibody; Calcium/calmodulin dependent serine protein kinase antibody; Calcium/calmodulin dependent serine protein kinase (MAGUK family) antibody; Calcium/calmodulin dependent serine protein kinase membrane associated guanylate kinase antibody; Calcium/calmodulin-dependent serine protein kinase antibody; CAMGUK antibody; CAMGUK protein antibody; CAMGUK, drosophila, homolog of antibody; casK antibody; CMG antibody; CSKP_HUMAN antibody; DXPri1 antibody; DXRib1 antibody; FGS4 antibody; FLJ22219 antibody; FLJ31914 antibody; hCASK antibody; LIN 2 antibody; Lin 2 homolog antibody; LIN2 antibody; Lin2 homolog antibody; MICPCH antibody; MRXSNA antibody; Pals3 antibody; Peripheral plasma membrane protein CASK antibody; Protein lin-2 homolog antibody; TNRC8 antibody; Trinucleotide repeat containing 8 antibody; Vertebtate LIN2 homolog antibody;
Documentation
[1]. Hsueh YP, et al. Direct interaction of CASK/LIN-2 and syndecan heparan sulfate proteoglycan and their overlapping distribution in neuronal synapses. J Cell Biol. 1998 Jul 13;142(1):139-51. [Content Brief]
[2]. Tabuchi K, et al. CASK participates in alternative tripartite complexes in which Mint 1 competes for binding with caskin 1, a novel CASK-binding protein. J Neurosci. 2002 Jun 1;22(11):4264-73. [Content Brief]
[3]. Silva B, et al. Interactions between amyloid precursor protein-like (APPL) and MAGUK scaffolding proteins contribute to appetitive long-term memory in Drosophila melanogaster. J Neurogenet. 2020 Mar;34(1):92-105. [Content Brief]
[4]. Schuh K, et al. Interaction of the plasma membrane Ca2+ pump 4b/CI with the Ca2+/calmodulin-dependent membrane-associated kinase CASK. J Biol Chem. 2003 Mar 14;278(11):9778-83. [Content Brief]
[5]. Atasoy D, et al. Deletion of CASK in mice is lethal and impairs synaptic function. Proc Natl Acad Sci U S A. 2007 Feb 13;104(7):2525-30. [Content Brief]
[6]. Pan YE, et al. Missense mutations in CASK, coding for the calcium-/calmodulin-dependent serine protein kinase, interfere with neurexin binding and neurexin-induced oligomerization. J Neurochem. 2021 May;157(4):1331-1350. [Content Brief]
[7]. Tibbe D, et al. Functional analysis of CASK transcript variants expressed in human brain. PLoS One. 2021 Jun 16;16(6):e0253223. [Content Brief]
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