Frizzled 1/2/7 Antibody
(Synonyms: FZD1, FZD2, FZD7, Frizzled-7, Fz-7, hFz7, FzE3)Frizzled 1/2/7 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Frizzled 1/2/7.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse, Rat, Chicken
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Formulation:
Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-5000 | 1:50-200 |
Product Details
Frizzled 1/2/7 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Frizzled 1/2/7.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat, Chicken
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Observed Molecular WeightObserved band size: 71 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 71,63 kDa
Synthesized peptide derived from human Frizzled 1/2/7
Endogenous
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Frizzled-1: Frizzled-1 is a Wnt protein receptor that can be activated by WNT3A, WNT3, and WNT1, and can also be activated by WNT2 (but with weaker efficacy), but not by WNT4, WNT5A, WNT5B, WNT6, WNT7A, or WNT7B. Frizzled-1 plays a role in the classical Wnt/β-catenin signaling pathway. The classical Wnt/β-catenin signaling pathway leads to the activation of Dishevelled proteins, the inhibition of GSK-3 kinase, the accumulation of β-catenin in the nucleus, and the activation of Wnt target genes[2].
Frizzled-2: Frizzled-2 is a Wnt protein receptor. Most Frizzled receptors are coupled to the classical β-catenin signaling pathway, which leads to the activation of Dishevelled proteins, the inhibition of GSK-3 kinase, the accumulation of β-catenin in the nucleus, and the activation of Wnt target genes[1].
Frizzled-7: Frizzled-7 is a Wnt protein receptor. Most Frizzled receptors are coupled to the classical β-catenin signaling pathway, which leads to the activation of Dishevelled proteins, inhibition of GSK-3 kinase, accumulation of β-catenin in the nucleus, and activation of Wnt target genes[1]. -
Subcellular Localization
Frizzled-1: Cell membrane; Multi-pass membrane protein
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Expression
Frizzled-1: Tissue specificity:Widely expressed. In the adult, mainly found in heart, placenta, skeletal muscle, lung, kidney, pancreas, prostate, testis, ovary and colon. In the fetus, expressed in brain, lung and kidney. Low levels in fetal liver. -
Isoforms & Post-Translational Modification
Frizzled-1: 647 amino acids, molecular weight 71,158 Da (predicted).
Frizzled-2: 565 amino acids, molecular weight 63,554 Da (predicted).
Frizzled-7: 574 amino acids, molecular weight 63,620 Da (predicted).
Frizzled-1/Frizzled-/Frizzled-7: Ubiquitinated by ZNRF3, leading to its degradation by the proteasome. -
Subunit
Frizzled-1: Interacts with MYOC.
Frizzled-2: Interacts with C.difficile toxin TcdB; frizzled receptors constitute the major host receptors for TcdB in the colonic epithelium.
Frizzled-7: Interacts with CCDC88C/DAPLE; the interaction displaces DVL1 from FZD7, leading to inhibition of canonical Wnt signaling and triggering of non-canonical Wnt responses. Interacts with MYOC. Binds to SDCBP; this interaction is increased by inositol trisphosphate (IP3). -
Synonyms
FZD1, FZD2, FZD7, Frizzled-7, Fz-7, hFz7, FzE3
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Research Field
Cell Biology
Documentation
References
[1]. Saal HM, et al. A mutation in FRIZZLED2 impairs Wnt signaling and causes autosomal dominant omodysplasia. Hum Mol Genet. 2015 Jun 15;24(12):3399-409. [Content Brief]
[2]. Gazit A, et al. Human frizzled 1 interacts with transforming Wnts to transduce a TCF dependent transcriptional response. Oncogene. 1999 Oct 28;18(44):5959-66. [Content Brief]
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