SMAD7 Antibody
(Synonyms: Mad homolog 7, hSMAD7, CRCS3, SMAD family member 7, )SMAD7 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to SMAD7.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IHC-F, ICC/IF, FC, IF-Tissue
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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FC
FC: Flow Cytometry
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|---|---|---|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:100-500 | 1:100-500 | 1:100-500 | 1:100-500 | 1ug/Test |
Product Details
SMAD7 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to SMAD7.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 46 kDa
KLH conjugated synthetic peptide derived from human Smad7 : 1-100/426
Endogenous
affinity purified by Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
SMAD7 is an Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator[1].
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Subcellular Localization
Nucleus; Cytoplasm
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Expression
Tissue_Specificity: Ubiquitous with higher expression in the lung and vascular endothelium.
Induction: By TGFB1. -
Isoforms & Post-Translational Modification
SMAD7 has 3 isoforms, O15105-1: amino acid length is 426, molecular weight is 46426 Da (predicted); O15105-2: amino acid length is 211, molecular weight is 23901 Da (predicted); O15105-3: amino acid length is 425, molecular weight is 46355 Da (predicted).
Phosphorylated by PDPK1. -
Subunit
Interacts with WWP1 (By similarity).
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SwissProt ID
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Synonyms
Mad homolog 7, hSMAD7, CRCS3, SMAD family member 7,
Documentation
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