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YTHDC1 Antibody (YA6841)

(Synonyms: KIAA1966 antibody; Putative splicing factor YT521 antibody; splicing factor YT521-B antibody; YT521 antibody; YT521-B antibody; YTDC1_HUMAN antibody; YTH domain-containing protein 1 antibody; YTHDC1 antibody; )
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Based on 1 Customer Validation

YTHDC1 Antibody (YA6841) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to YTHDC1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for YTHDC1 Antibody (YA6841)
  • YTHDC1 Antibody (YA6841) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000 1:50 1:1000

Product Details

Description

YTHDC1 Antibody (YA6841) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to YTHDC1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 100 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 85 kDa
Immunogen

Recombinant protein within human YTHDC1 aa 1-550.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for YTHDC1 Antibody (YA6841)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HEK293 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using YTHDC1 Antibody (HY-P87148) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    YTHDC1 is a nuclear m⁶A RNA reader that selectively binds m⁶A-containing RNA through its YTH domain and preferentially recognizes GG (m⁶A) C sequences[1]. Mechanistically, YTHDC1 controls nuclear RNA metabolism by promoting mRNA splicing and by mediating export of methylated mRNAs from the nucleus to the cytoplasm through SRSF3 and NXF1[2][3]. In AML models, YTHDC1 supports leukemia cell proliferation, survival, AML development, and leukemia stem cell self-renewal through an MCM4 DNA-replication mechanism[4]. Compared with related isoforms, YTHDC1 functions as the only nuclear YTH-family m⁶A-binding protein in this export pathway, whereas YTHDF2 mainly mediates cytoplasmic decay of methylated mRNAs and YTHDC2 has RNA helicase activity essential for mammalian germline meiosis[2][5]. For experimental applications, early YTHDC1 inhibitors that occupy the RNA-binding pocket disrupt YTHDC1-m⁶A RNA interaction, dissolve YTHDC1 condensates, and suppress oncogenic gene expression in AML cell lines[6].

  • Subcellular Localization

    Nucleus,Nucleus speckle

  • Isoforms & Post-Translational Modification

    Q96MU7 has two isomers: Q96MU7-1: 84700 Da (predicted); Q96MU7-2: 82692 Da (predicted).
    Tyrosine phosphorylated

  • Subunit

    Interacts with SRSF1 (By similarity)

  • SwissProt ID

    Q96MU7

  • Gene ID
    91746 [NCBI]
  • Synonyms

    KIAA1966 antibody; Putative splicing factor YT521 antibody; splicing factor YT521-B antibody; YT521 antibody; YT521-B antibody; YTDC1_HUMAN antibody; YTH domain-containing protein 1 antibody; YTHDC1 antibody;

[1]. Xu C, et al. Structural basis for selective binding of m6A RNA by the YTHDC1 YTH domain. Nat Chem Biol. 2014 Nov;10(11):927-9. [Content Brief]

[2]. Roundtree IA, et al. YTHDC1 mediates nuclear export of N6-methyladenosine methylated mRNAs. Elife. 2017 Oct 6;6:e31311. [Content Brief]

[3]. Roundtree IA, et al. Nuclear m(6)A Reader YTHDC1 Regulates mRNA Splicing. Trends Genet. 2016 Jun;32(6):320-321. [Content Brief]

[4]. Sheng Y, et al. A critical role of nuclear m6A reader YTHDC1 in leukemogenesis by regulating MCM complex-mediated DNA replication. Blood. 2021 Dec 30;138(26):2838-2852. [Content Brief]

[5]. Li L, et al. The XRN1-regulated RNA helicase activity of YTHDC2 ensures mouse fertility independently of m6A recognition. Mol Cell. 2022 May 5;82(9):1678-1690.e12. [Content Brief]

[6]. Ali SR, et al. Nerve Density and Neuronal Biomarkers in Cancer. Cancers (Basel). 2022 Oct 1;14(19):4817. [Content Brief]

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YTHDC1 Antibody (YA6841) Related Classifications

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Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HEK293 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using YTHDC1 Antibody (HY-P87148) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • YTHDC1 Antibody (YA6841) Product Image
    1/16
  • Experimental Validation Results for YTHDC1 Antibody (YA6841)
  • YTHDC1 Antibody (YA6841) Product Image
  • Experimental Validation Results for YTHDC1 Antibody (YA6841)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HEK293 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using YTHDC1 Antibody (HY-P87148) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for YTHDC1 Antibody (YA6841)
  • YTHDC1 Antibody (YA6841) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HEK293 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using YTHDC1 Antibody (HY-P87148) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • YTHDC1 Antibody (YA6841) Product Image WB