Cytochrome C Antibody (YA6421)
(Synonyms: CytC; CYC; CYCS; Cytochrome c somatic; HCS; CYC_HUMAN; Cytochrome c; MSA06; THC4.)
Customer Review
Based on 1 Customer Validation
Cytochrome C Antibody (YA6421) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to Cytochrome C.
For research use only. We do not sell to patients.
-
Host:
Mouse
-
Isotype:
IgG
-
Application:
WB, IHC-P, IHC-F, ICC/IF, IF-Tissue
-
Reactivity :
Human, Mouse, Rat
-
Formulation:
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
-
Conjugation:
Non-conjugated
ALL
WB
IHC-P
mIHC
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Service
Applications
| Application |
WB
WB: Western Blot
|
IHC-P
IHC-P: Immunohistochemistry-Paraffin
|
IHC-F
IHC-F: Immunohistochemistry-Frozen
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
|
|---|---|---|---|---|---|
| Dilution Ratio | 1:2000-1:20000 | 1:200-1:800 | 1:200-1:800 | 1:100-1:500 | 1:200-1:800 |
Product Details
Description
Cytochrome C Antibody (YA6421) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to Cytochrome C.
-
Host Mouse
-
Clonality Monoclonal
-
Species ReactivityHuman, Mouse, Rat
-
Observed Molecular WeightObserved band size: 15 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 12 kDa
Immunogen
Recombinant human Cytochrome C Protein
Purification
affinity purified.
Conjugation
Non-conjugated
Modification
Unmodified
Isotype
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
-
Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Verification Images
-
Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), A549 (lane 3), Mouse muscle (lane 4), Mouse heart (lane 5), Mouse kidney (lane 6), and Rat kidney (lane 7) using Cytochrome C antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005). -
Immunohistochemical analysis of paraffin-embedded human colon cancer using Cytochrome C antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86729, 1/800) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human ovarian cancer using Cytochrome C antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86729, 1/800) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human bladder cancer using Cytochrome C antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86729, 1/800) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human thyroid cancer using Cytochrome C antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86729, 1/800) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver using Cytochrome C antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86729, 1/800) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human glioma using Cytochrome C antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86729, 1/800) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human non-small cell lung cancer using Cytochrome C antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86729, 1/800) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human bladder cancer using Cytochrome C antibody. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate (pH 6.0) for 14 minutes. The tissues were blocked in 5% BSA for 10 minutes at room temperature and then probed with the primary antibody (HY-P86729, 1/800) for 2 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). Fluorescence was then developed with Vari Fluor 488 TSA(200×) (HY-D1837). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium (HY-K1047). -
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human liver cancer using Cytochrome C antibody. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate (pH 6.0) for 14 minutes. The tissues were blocked in 5% BSA for 10 minutes at room temperature and then probed with the primary antibody (HY-P86729, 1/800) for 2 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). Fluorescence was then developed with Vari Fluor 488 TSA(200×) (HY-D1837). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium (HY-K1047). -
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human liver using Cytochrome C antibody. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate (pH 6.0) for 14 minutes. The tissues were blocked in 5% BSA for 10 minutes at room temperature and then probed with the primary antibody (HY-P86729, 1/800) for 2 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). Fluorescence was then developed with Vari Fluor 488 TSA(200×) (HY-D1837). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium (HY-K1047). -
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human cervical cancer using Cytochrome C antibody. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate (pH 6.0) for 14 minutes. The tissues were blocked in 5% BSA for 10 minutes at room temperature and then probed with the primary antibody (HY-P86729, 1/800) for 2 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). Fluorescence was then developed with Vari Fluor 488 TSA(200×) (HY-D1837). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium (HY-K1047). -
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human colon cancer using Cytochrome C antibody. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate (pH 6.0) for 14 minutes. The tissues were blocked in 5% BSA for 10 minutes at room temperature and then probed with the primary antibody (HY-P86729, 1/800) for 2 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). Fluorescence was then developed with Vari Fluor 488 TSA(200×) (HY-D1837). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium (HY-K1047). -
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarain cancer using Cytochrome C antibody. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate (pH 6.0) for 14 minutes. The tissues were blocked in 5% BSA for 10 minutes at room temperature and then probed with the primary antibody (HY-P86729, 1/800) for 2 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). Fluorescence was then developed with Vari Fluor 488 TSA(200×) (HY-D1837). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium (HY-K1047). -
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human prostate using Cytochrome C antibody. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate (pH 6.0) for 14 minutes. The tissues were blocked in 5% BSA for 10 minutes at room temperature and then probed with the primary antibody (HY-P86729, 1/800) for 2 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). Fluorescence was then developed with Vari Fluor 488 TSA(200×) (HY-D1837). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium (HY-K1047). -
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human prostate cancer using Cytochrome C antibody. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate (pH 6.0) for 14 minutes. The tissues were blocked in 5% BSA for 10 minutes at room temperature and then probed with the primary antibody (HY-P86729, 1/800) for 2 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). Fluorescence was then developed with Vari Fluor 488 TSA(200×) (HY-D1837). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium (HY-K1047). -
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human kidney using Cytochrome C antibody. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate (pH 6.0) for 14 minutes. The tissues were blocked in 5% BSA for 10 minutes at room temperature and then probed with the primary antibody (HY-P86729, 1/800) for 2 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). Fluorescence was then developed with Vari Fluor 488 TSA(200×) (HY-D1837). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium (HY-K1047). -
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human stomach cancer using Cytochrome C antibody. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate (pH 6.0) for 14 minutes. The tissues were blocked in 5% BSA for 10 minutes at room temperature and then probed with the primary antibody (HY-P86729, 1/800) for 2 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). Fluorescence was then developed with Vari Fluor 488 TSA(200×) (HY-D1837). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium (HY-K1047). -
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human pancreas using Cytochrome C antibody. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate (pH 6.0) for 14 minutes. The tissues were blocked in 5% BSA for 10 minutes at room temperature and then probed with the primary antibody (HY-P86729, 1/800) for 2 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). Fluorescence was then developed with Vari Fluor 488 TSA(200×) (HY-D1837). The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium (HY-K1047).
Background
-
Function
Cytochrome C is an Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain|Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases
-
Subcellular Localization
Mitochondrion intermembrane space
-
Isoforms & Post-Translational Modification
P99999: 105 amino acids, molecular weight 11749 Da.
-
SwissProt ID
-
Synonyms
CytC; CYC; CYCS; Cytochrome c somatic; HCS; CYC_HUMAN; Cytochrome c; MSA06; THC4.
Documentation
Select Batch:
-
Data Sheet (231 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
User Guide for Antibodies (1077 KB)
Powered by Bioz
