Mast Cell Tryptase Antibody (YA1533)

Cat. No.: HY-P81788: Data Sheet COA
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Mast Cell Tryptase Antibody (YA1533) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Mast Cell Tryptase.

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10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
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Mast Cell Tryptase Antibody (YA1533) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
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Mast Cell Tryptase Antibody (YA1533) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Mast Cell Tryptase.

Host

Rabbit

Clonality

Recombinant,Monoclonal

분자량

Predicted band size: 31 kDa;

Observed band size: 31 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q15661

Gene ID

7177 [NCBI]

Immunogen

A synthesized peptide derived from human Mast Cell Tryptase aa10-275.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IP IP: Immunoprecipitation	1:50

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

ALL WB IHC-P

Western blot analysis was performed on extracts from U937 (lane 1, 15 μg), and Mouse liver (lane 2, 15 μg) using Tryptase I Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue using Mast Cell Tryptase Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue using Mast Cell Tryptase Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates (PubMed:18854315)

Subcellular Localization：Secreted

Expression：
Tissue_specificity:Isomer 1 and isomer 2 are expressed in the lungs, stomach, spleen, heart, and skin; in these tissues, isomer 1 is dominant. Isomer 2 is expressed in aortic, splenic, and breast tumors, with the highest expression levels in endothelial cells of certain vessels around the aorta and in endothelial cells around tumors, while expression levels are very low (at the protein level) in mast cells.

Isoforms & Post-Translational Modification：Q15661 has 2 isomers: Q15661-1: 30515 Da (predicted); Q15661-2: 29533 Da (predicted).

Subunit：Homotetramer. The active tetramer is converted to inactive monomers at neutral and acidic pH in the absence of heparin. Low concentrations of inactive monomers become active monomers at pH 6.0 in the presence of heparin. When the concentration of active monomers is higher, they convert to active monomers and then to active tetramers. These monomers are active and functionally distinct from the tetrameric enzyme. In contrast to the hidden active sites in the tetrameric form, the active site of the monomeric form is accessible for macromolecular proteins and inhibitors, e.g. fibrinogen which is a substrate for the monomeric but not for the tetrameric form. The monomeric form forms a complex with SERPINB6

RRID

AB_3103891

Database

Entrez Gene: 7177 Human ; 100503895 Mouse ; 54271 Rat

SwissProt: Q15661 Human ; Q02844 Mouse ; P27435 Rat

OMIM: 191080 Human

Research Field

Immunology

Synonyms

TPS1; TPS2; TPSAB1; TPSB1; MCP 7; Mast cell protease 7; Tryptase alpha 1

각종 서류

Select Batch:

Data Sheet (235 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/a-375-cell.html [Content Brief]

Mast Cell Tryptase Antibody (YA1533) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Mast Cell Tryptase Antibody (YA1533)

Mast Cell Tryptase Antibody (YA1533) Featured Recommendations:

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Mast Cell Tryptase Antibody (YA1533) Related Classifications

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