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  5. E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody (YA1817)

E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody (YA1817)

Art. -Nr.: HY-P82072
COA
SDS
User Guide for Antibodies Technical Support

E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody (YA1817) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to E1 Ubiquitin Activating Enzyme 1/UBA1.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Beschreibung

Beschreibung

E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody (YA1817) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to E1 Ubiquitin Activating Enzyme 1/UBA1.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Molekulargewicht
Predicted band size: 118 kDa;
Observed band size: 118 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human E1 Ubiquitin Activating Enzyme aa200-255/1058.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:1000-1:2000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:100-1:200
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
FC
FC: Flow Cytometry
1:50-1:100
Sensitivity Endogenous Reinheit Affinity Chromatography
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Versand

Shipping with blue ice.
Background
Function：Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system (PubMed:1447181, PubMed:1606621, PubMed:33108101). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181). Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334)
Subcellular Localization：Cytoplasm; Mitochondrion; Nucleus; Nucleus; Cytoplasm
Expression：
Tissue_specificity:Detected in red blood cells (protein level) . Widely present.
Isoforms & Post-Translational Modification：P22314 has 2 isomers: P22314-1: 117849 Da (predicted); P22314-2: 113800 Da (predicted).
ISGylated
Subunit：Monomer (By similarity). Interacts with GAN (via BTB domain)
Database

Entrez Gene: 7317 Human ; 22201 Mouse ; 314432 Rat

SwissProt: P22314 Human ; Q02053 Mouse ; Q5U300 Rat

OMIM: 301830 Human

Research Field

Cell Biology
Synonyms
UBA1; A1S9T; UBE1; Ubiquitin-like modifier-activating enzyme 1; Protein A1S9; Ubiquitin-activating enzyme E1
Dokumentation
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody (YA1817) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody (YA1817)
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