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E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody (YA1817)

(Synonyms: UBA1; A1S9T; UBE1; Ubiquitin-like modifier-activating enzyme 1; Protein A1S9; Ubiquitin-activating enzyme E1)
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Based on 1 Customer Validation

E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody (YA1817) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to E1 Ubiquitin Activating Enzyme 1/UBA1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody (YA1817)
  • E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody (YA1817) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:2000 1:100-1:200 1:50-1:200 1:50-1:100

Product Details

Description

E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody (YA1817) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to E1 Ubiquitin Activating Enzyme 1/UBA1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 118 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 118 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human E1 Ubiquitin Activating Enzyme aa200-255/1058.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody (YA1817)
    Flow cytometric analysis of 1X106 K562 cells labeling UBA1 Antibody (HY-P82072, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    E1 Ubiquitin Activating Enzyme 1/UBA1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites[1][2][3][4].

  • Subcellular Localization

    Cytoplasm; Mitochondrion; Nucleus; Nucleus; Cytoplasm

  • Expression


    Tissue_specificity:Detected in red blood cells (protein level) . Widely present.

  • Isoforms & Post-Translational Modification

    P22314 has 2 isomers: P22314-1: 117849 Da (predicted); P22314-2: 113800 Da (predicted).
    ISGylated

  • Subunit

    Monomer (By similarity). Interacts with GAN (via BTB domain)

  • SwissProt ID

    P22314

  • Gene ID
    7317 [NCBI]
  • Synonyms

    UBA1; A1S9T; UBE1; Ubiquitin-like modifier-activating enzyme 1; Protein A1S9; Ubiquitin-activating enzyme E1

  • Research Field

    Cell Biology

References

[1]. Cook JC, et al. Isoforms of mammalian ubiquitin-activating enzyme. J Biol Chem. 1992 Dec 5;267(34):24315-21. [Content Brief]

[2]. Ayusawa D, et al. Complementation by a cloned human ubiquitin-activating enzyme E1 of the S-phase-arrested mouse FM3A cell mutant with thermolabile E1. Cell Struct Funct. 1992 Apr;17(2):113-22. [Content Brief]

[3]. Beck DB, et al. Somatic Mutations in UBA1 and Severe Adult-Onset Autoinflammatory Disease. N Engl J Med. 2020 Dec 31;383(27):2628-2638. [Content Brief]

[4]. Moudry P, et al. Ubiquitin-activating enzyme UBA1 is required for cellular response to DNA damage. Cell Cycle. 2012 Apr 15;11(8):1573-82. [Content Brief]

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E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody (YA1817) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 K562 cells labeling UBA1 Antibody (HY-P82072, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody (YA1817) Product Image
    1/16
  • Experimental Validation Results for E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody (YA1817)
  • E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody (YA1817) Product Image
  • Experimental Validation Results for E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody (YA1817)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 K562 cells labeling UBA1 Antibody (HY-P82072, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody (YA1817)
  • E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody (YA1817) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 K562 cells labeling UBA1 Antibody (HY-P82072, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • E1 Ubiquitin Activating Enzyme 1/UBA1 Antibody (YA1817) Product Image FC