Cullin 5 Antibody (YA2585)(PBS only)
(Synonyms: CUL-5; VACM1; VACM-1)Cullin 5 Antibody (YA2585) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cullin 5.
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB
-
Reactivity :
Human, Mouse, Rat
-
Formulation:
Supplied in PBS, pH 7.4.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
|---|---|
| Dilution Ratio | 1:500-1:1000 |
Product Details
Cullin 5 Antibody (YA2585) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cullin 5.
-
Host Rabbit
-
Species ReactivityHuman, Mouse, Rat
-
Observed Molecular WeightObserved band size: 91 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 91 kDa
Recombinant protein of human Cullin 5
Affinity Purified
Non-conjugated
IgG
Product Properties
-
Appearance
Liquid
-
Formulation
Supplied in PBS, pH 7.4.
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
Cullin 5 core component of multiple cullin-5-RING E3 ubiquitin-protein ligase complexes (ECS complexes, also named CRL5 complexes), which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Acts a scaffold protein that contributes to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable SOCS box-containing substrate recognition component. Acts as a key regulator of neuron positioning during cortex development: component of various SOCS-containing ECS complexes, such as the ECS(SOCS7) complex, that regulate reelin signaling by mediating ubiquitination and degradation of DAB1. ECS(SOCS1) seems to direct ubiquitination of JAK2. The ECS(SOCS2) complex mediates the ubiquitination and subsequent proteasomal degradation of phosphorylated EPOR and GHR. The ECS(SPSB3) complex catalyzes ubiquitination of nuclear CGAS. ECS(KLHDC1) complex is part of the DesCEND (destruction via C-end degrons) pathway and mediates ubiquitination and degradation of truncated SELENOS selenoprotein produced by failed UGA/Sec decoding, which ends with a glycine. The ECS(ASB9) complex mediates ubiquitination and degradation of CKB. As part of some ECS complex, promotes 'Lys-11'-linked ubiquitination and degradation of BTRC. As part of a multisubunit ECS complex, polyubiquitinates monoubiquitinated POLR2A. As part of the ECS(RAB40C) complex, mediates ANKRD28 ubiquitination and degradation, thereby inhibiting protein phosphatase 6 (PP6) complex activity and focal adhesion assembly during cell migration. As part of the ECS(RAB40A) complex, mediates RHOU 'Lys-48'-linked ubiquitination and degradation, thus inhibiting focal adhesion disassembly during cell migration. As part of the ECS(RAB40B) complex, mediates LIMA1/EPLIN and RAP2 ubiquitination, thereby regulating actin cytoskeleton dynamics and stress fiber formation during cell migration. May form a cell surface vasopressin receptor; (Microbial infection) Following infection by HIV-1 virus, CUL5 associates with HIV-1 Vif proteins and forms a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex) that catalyzes ubiquitination and degradation of APOBEC3F and APOBEC3G. The complex can also ubiquitinate APOBEC3H to some extent; (Microbial infection) Seems to be involved in proteasomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19].
-
Subcellular Localization
Nucleus
-
Subunit
Component of multiple cullin-5-RING E3 ubiquitin-protein ligase complexes (ECS complexes, also named CRL5 complexes) formed of CUL5, Elongin BC (ELOB and ELOC), RNF7/RBX2 and a variable SOCS box domain-containing protein as substrate-specific recognition component (PubMed:11384984, PubMed:15601820, PubMed:16325183, PubMed:21119685, PubMed:27910872, PubMed:32513959, PubMed:33268465, PubMed:35512830, PubMed:26598620, PubMed:33999101, PubMed:35293963). CUL5-containing ECS complexes specifically contain RNF7/RBX2, and not RBX1, as catalytic subunit (PubMed:11384984, PubMed:15601820, PubMed:32513959, PubMed:33268465). Component of the ECS(ASB2) complex with the substrate recognition component ASB2 (PubMed:16325183, PubMed:21119685). Component of the ECS(ASB6) complex with the substrate recognition component ASB6 (PubMed:16325183). Component of the ECS(ASB7) complex with the substrate recognition component ASB7 (PubMed:16325183). Component of the ECS(ASB9) complex with the substrate recognition component ASB9 (PubMed:33268465). Component of the ECS(ASB11) complex with the substrate recognition component ASB11 (PubMed:24337577, PubMed:31387940, PubMed:38574733). Component of the ECS(ASB12) complex with the substrate recognition component ASB12 (PubMed:16325183). Component of the ECS(LRRC41) complex with the substrate recognition component LRRC41 (PubMed:11384984). Component of the ECS(SOCS1) complex with the substrate recognition component SOCS1 (PubMed:11384984). Component of the ECS(SOCS2) complex with the substrate recognition component SOCS2 (PubMed:21980433, PubMed:23897481, PubMed:25505247). Component of the ECS(WSB1) complex with the substrate recognition subunit WSB1 (PubMed:15601820). Component of the ECS(SOCS3) complex with the substrate recognition component SOCS3 (PubMed:15601820). Component of the ECS(SOCS7) complex with the substrate recognition component SOCS7 (By similarity). Component of the ECS(SPSB1) complex with the substrate recognition component SPSB1 (PubMed:15601820). Component of the ECS(SPSB3) complex with the substrate recognition component SPSB3 (PubMed:38418882). Component of the ECS(SPSB2) complex with the substrate recognition component SPSB2 (PubMed:15601820). Component of the ECS(SPSB4) complex with the substrate recognition component SPSB4 (PubMed:15601820). Component of the ECS(RAB40) complex with the substrate recognition subunit RAB40A, RAB40B or RAB40C (PubMed:15601820, PubMed:35512830, PubMed:26598620, PubMed:33999101, PubMed:35293963). Component of the ECS(KLHDC1) complex with the substrate recognition component KLHDC1 (PubMed:32200094). Component of the ECS(PCMTD1) complex with the substrate recognition subunit PCMTD1 (PubMed:35486881). May also form complexes containing RBX1 and ELOA or VHL; additional evidence is however required to confirm this result in vivo (PubMed:10230407, PubMed:11384984, PubMed:18805092, PubMed:19920177). Interacts (when neddylated) with ARIH2; leading to activate the E3 ligase activity of ARIH2 (PubMed:24076655, PubMed:33268465, PubMed:34518685). Interacts with ERCC6; the interaction is induced by DNA damaging agents or inhibitors of RNA polymerase II elongation (PubMed:28292928). Interacts with ELOA (via the BC-box) (PubMed:28292928). Interacts (unneddylated form) with DCUN1D1, DCUN1D2, DCUN1D3, DCUN1D4 and DCUN1D5; these interactions promote the cullin neddylation (PubMed:23201271, PubMed:26906416)
-
SwissProt ID
-
Synonyms
CUL-5; VACM1; VACM-1
-
Research Field
Cell Biology
Documentation
Powered by Bioz
