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  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. Cullin 5 Antibody (YA2585)(PBS only)

Cullin 5 Antibody (YA2585)(PBS only)

Cat. No.: HY-P82840A
User Guide for Antibodies Technical Support

Cullin 5 Antibody (YA2585) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cullin 5.

For research use only. We do not sell to patients.

Size Stock
50 μL   Get quote  
100 μL   Get quote  

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Documentation

Description

Cullin 5 Antibody (YA2585) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cullin 5.
Host

Rabbit
Molecular Weight
Predicted band size: 91 kDa;
Observed band size: 91 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

Recombinant protein of human Cullin 5
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
Purity Affinity Purified Conjugation Non-conjugated
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in PBS, pH 7.4.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Background
Function：Core component of multiple cullin-5-RING E3 ubiquitin-protein ligase complexes (ECS complexes, also named CRL5 complexes), which mediate the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:11384984, PubMed:15601820, PubMed:21199876, PubMed:21980433, PubMed:23897481, PubMed:25505247, PubMed:27910872, PubMed:32200094, PubMed:33268465, PubMed:35512830, PubMed:38418882). Acts a scaffold protein that contributes to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme (PubMed:11384984, PubMed:15601820, PubMed:33268465). The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable SOCS box-containing substrate recognition component (PubMed:11384984, PubMed:15601820, PubMed:33268465). Acts as a key regulator of neuron positioning during cortex development: component of various SOCS-containing ECS complexes, such as the ECS(SOCS7) complex, that regulate reelin signaling by mediating ubiquitination and degradation of DAB1 (By similarity). ECS(SOCS1) seems to direct ubiquitination of JAK2 (PubMed:11384984). The ECS(SOCS2) complex mediates the ubiquitination and subsequent proteasomal degradation of phosphorylated EPOR and GHR (PubMed:21980433, PubMed:25505247). The ECS(SPSB3) complex catalyzes ubiquitination of nuclear CGAS (PubMed:38418882). ECS(KLHDC1) complex is part of the DesCEND (destruction via C-end degrons) pathway and mediates ubiquitination and degradation of truncated SELENOS selenoprotein produced by failed UGA/Sec decoding, which ends with a glycine (PubMed:32200094). The ECS(ASB9) complex mediates ubiquitination and degradation of CKB (PubMed:33268465). As part of some ECS complex, promotes 'Lys-11'-linked ubiquitination and degradation of BTRC (PubMed:27910872). As part of a multisubunit ECS complex, polyubiquitinates monoubiquitinated POLR2A (PubMed:19920177). As part of the ECS(RAB40C) complex, mediates ANKRD28 ubiquitination and degradation, thereby inhibiting protein phosphatase 6 (PP6) complex activity and focal adhesion assembly during cell migration (PubMed:35512830). As part of the ECS(RAB40A) complex, mediates RHOU 'Lys-48'-linked ubiquitination and degradation, thus inhibiting focal adhesion disassembly during cell migration (PubMed:26598620). As part of the ECS(RAB40B) complex, mediates LIMA1/EPLIN and RAP2 ubiquitination, thereby regulating actin cytoskeleton dynamics and stress fiber formation during cell migration (PubMed:33999101, PubMed:35293963). May form a cell surface vasopressin receptor (PubMed:9037604); (Microbial infection) Following infection by HIV-1 virus, CUL5 associates with HIV-1 Vif proteins and forms a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex) that catalyzes ubiquitination and degradation of APOBEC3F and APOBEC3G (PubMed:16636053, PubMed:22190037). The complex can also ubiquitinate APOBEC3H to some extent (PubMed:37640699); (Microbial infection) Seems to be involved in proteasomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein
Subcellular Localization：Nucleus
Subunit：Component of multiple cullin-5-RING E3 ubiquitin-protein ligase complexes (ECS complexes, also named CRL5 complexes) formed of CUL5, Elongin BC (ELOB and ELOC), RNF7/RBX2 and a variable SOCS box domain-containing protein as substrate-specific recognition component (PubMed:11384984, PubMed:15601820, PubMed:16325183, PubMed:21119685, PubMed:27910872, PubMed:32513959, PubMed:33268465, PubMed:35512830, PubMed:26598620, PubMed:33999101, PubMed:35293963). CUL5-containing ECS complexes specifically contain RNF7/RBX2, and not RBX1, as catalytic subunit (PubMed:11384984, PubMed:15601820, PubMed:32513959, PubMed:33268465). Component of the ECS(ASB2) complex with the substrate recognition component ASB2 (PubMed:16325183, PubMed:21119685). Component of the ECS(ASB6) complex with the substrate recognition component ASB6 (PubMed:16325183). Component of the ECS(ASB7) complex with the substrate recognition component ASB7 (PubMed:16325183). Component of the ECS(ASB9) complex with the substrate recognition component ASB9 (PubMed:33268465). Component of the ECS(ASB11) complex with the substrate recognition component ASB11 (PubMed:24337577, PubMed:31387940, PubMed:38574733). Component of the ECS(ASB12) complex with the substrate recognition component ASB12 (PubMed:16325183). Component of the ECS(LRRC41) complex with the substrate recognition component LRRC41 (PubMed:11384984). Component of the ECS(SOCS1) complex with the substrate recognition component SOCS1 (PubMed:11384984). Component of the ECS(SOCS2) complex with the substrate recognition component SOCS2 (PubMed:21980433, PubMed:23897481, PubMed:25505247). Component of the ECS(WSB1) complex with the substrate recognition subunit WSB1 (PubMed:15601820). Component of the ECS(SOCS3) complex with the substrate recognition component SOCS3 (PubMed:15601820). Component of the ECS(SOCS7) complex with the substrate recognition component SOCS7 (By similarity). Component of the ECS(SPSB1) complex with the substrate recognition component SPSB1 (PubMed:15601820). Component of the ECS(SPSB3) complex with the substrate recognition component SPSB3 (PubMed:38418882). Component of the ECS(SPSB2) complex with the substrate recognition component SPSB2 (PubMed:15601820). Component of the ECS(SPSB4) complex with the substrate recognition component SPSB4 (PubMed:15601820). Component of the ECS(RAB40) complex with the substrate recognition subunit RAB40A, RAB40B or RAB40C (PubMed:15601820, PubMed:35512830, PubMed:26598620, PubMed:33999101, PubMed:35293963). Component of the ECS(KLHDC1) complex with the substrate recognition component KLHDC1 (PubMed:32200094). Component of the ECS(PCMTD1) complex with the substrate recognition subunit PCMTD1 (PubMed:35486881). May also form complexes containing RBX1 and ELOA or VHL; additional evidence is however required to confirm this result in vivo (PubMed:10230407, PubMed:11384984, PubMed:18805092, PubMed:19920177). Interacts (when neddylated) with ARIH2; leading to activate the E3 ligase activity of ARIH2 (PubMed:24076655, PubMed:33268465, PubMed:34518685). Interacts with ERCC6; the interaction is induced by DNA damaging agents or inhibitors of RNA polymerase II elongation (PubMed:28292928). Interacts with ELOA (via the BC-box) (PubMed:28292928). Interacts (unneddylated form) with DCUN1D1, DCUN1D2, DCUN1D3, DCUN1D4 and DCUN1D5; these interactions promote the cullin neddylation (PubMed:23201271, PubMed:26906416)
Research Field

Cell Biology
Synonyms
CUL-5; VACM1; VACM-1
Documentation
User Guide for Antibodies (1077 KB)

Cullin 5 Antibody (YA2585)(PBS only) Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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