HspA12A Antibody (YA2947)

Cat. No.: HY-P83202: Ficha de datos COA
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HspA12A Antibody (YA2947) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HspA12A.

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Tamaño	Stock	Cantidad
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10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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HspA12A Antibody (YA2947) Recomendaciones destacadas:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Descripciòn

Descripciòn

HspA12A Antibody (YA2947) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HspA12A.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Peso molecular

Predicted band size: 75 kDa;

Observed band size: 75 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O43301

Gene ID

259217 [NCBI]

Immunogen

A synthesized peptide derived from human HSPA12A aa104-143.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:50-1:100

Sensitivity	Endogenous	Pureza	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

ALL WB ICC

Western blot analysis of extracts from U-87MG(lane 2(20ug) and U-87MG(lane 3(40ug) using HspA12A Antibody (HY-P83202) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of A549 cells labeling HspA12A Antibody (HY-P83202) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with HspA12A Antibody (HY-P83202) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of A549 cells labeling HspA12A Antibody (HY-P83202) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with HspA12A Antibody (HY-P83202) at 1/150 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Adapter protein for SORL1, but not SORT1. Delays SORL1 internalization and affects SORL1 subcellular localization

Subcellular Localization：Cytoplasm; Nucleus

Expression：
Tissue_specificity:Widely expressed with highest levels in brain, kidney and muscle

Subunit：Interacts with SORL1 (via cytosolic C-terminus); this interaction affects SORL1 internalization and subcellular localization

RRID

AB_3105317

Database

Entrez Gene: 259217 Human ; 73442 Mouse ; 307997 Rat

SwissProt: O43301 Human ; Q8K0U4 Mouse ;

OMIM: 610701 Human

Research Field

Signal Transduction

Synonyms

FLJ13874 antibody; Heat shock 70 kDa protein 12A antibody; heat shock 70kD protein 12A antibody; heat shock 70kDa protein 12A antibody; HS12A_HUMAN antibody; Hspa12a antibody; KIAA0417 antibody

Documentación

Select Batch:

Ficha de datos (232 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

HspA12A Antibody (YA2947) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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HspA12A Antibody (YA2947)

HspA12A Antibody (YA2947) Recomendaciones destacadas:

Product Detail

Verification Image

Background

Descripciòn

HspA12A Antibody (YA2947) Related Classifications

Productos vistos recientemente:

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