Copy Link Print Information

TCF-4/TCF7L2 Antibody (YA2167)

(Synonyms: TCF7L2; HMG box transcription factor 4; HTCF-4; T-cell factor-4 variant X2; T-cell factor 4; Transcription factor 7-like 2; TCF-4)
Customer Review

Based on 1 Customer Validation

TCF-4/TCF7L2 Antibody (YA2167) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TCF-4/TCF7L2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA2167)
  • TCF-4/TCF7L2 Antibody (YA2167) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC Info
 
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:5000 P-1:100-1:200 1:100-1:200 1:50-1:100

Product Details

Description

TCF-4/TCF7L2 Antibody (YA2167) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TCF-4/TCF7L2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 68 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 68 kDa
Species Reactivity Database

Entrez Gene: 6934 Human

SwissProt: Q9NQB0 Human

Immunogen

A synthetic peptide of human TCF-4 aa2-14.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA2167)
    Immunohistochemical analysis of paraffin-embedded human lung cancer using TCF-4/TCF7L2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82422, 1/100) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    TCF-4/TCF7L2 participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as a repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine

  • Subcellular Localization

    Nucleus, PML body; Nucleus

  • Expression


    Tissue_specificity:This gene was detected in small intestinal epithelial cells, with the highest expression at the top of the crypts and a gradient of expression from the crypts to the villi. It was also detected in colonic epithelial cells and colon cancer, as well as in breast epithelial cells and their derivative carcinomas.

  • Isoforms & Post-Translational Modification

    Q9NQB0 has 17 isomers: Q9NQB0-1: 67919 Da (predicted); Q9NQB0-2: 55152 Da (predicted); Q9NQB0-3: 67976 Da (predicted); Q9NQB0-4: 53270 Da (predicted); Q9NQB0-5: 66037 Da (predicted); Q9NQB0-6: 53676 Da (predicted); Q9NQB0-7: 66094 Da (predicted); Q9NQB0-8: 65291 Da (predicted); Q9NQB0-9: 51794 Da (predicted); Q9NQB0-10: 50611 Da (predicted); Q9NQB0-11: 54572 Da (predicted); Q9NQB0-12: 51159 Da (predicted); Q9NQB0-13: 63466 Da (predicted); Q9NQB0-14: 49166 Da (predicted); Q9NQB0-15: 49688 Da (predicted); Q9NQB0-16: 63930 Da (predicted); Q9NQB0-17: 36884 Da (predicted).
    In vitro, phosphorylated by TNIK;Phosphorylated at Thr-201 and/or Thr-212 by NLK. Phosphorylation by NLK at these sites inhibits DNA-binding by TCF7L2/TCF4, thereby preventing transcriptional activation of target genes of the canonical Wnt/beta-catenin signaling pathway;Polysumoylated. Sumoylation is enhanced by PIAS family members and desumoylation is enhanced by SENP2. Sumoylation/desumoylation regulates TCF7L2/TCF4 transcription activity in the Wnt/beta-catenin signaling pathway without altering interaction with CTNNB1 nor binding to DNA

  • Subunit

    Interacts with TGFB1I1 (By similarity). Interacts with CTNNB1 (via the armadillo repeat); forms stable transcription complex (PubMed:10080941, PubMed:17052462, PubMed:19816403, PubMed:28829046, PubMed:29739711, PubMed:9065401, PubMed:9916915). Interacts with EP300. Interacts with NLK. Interacts with CCDC85B (probably through the HMG box); prevents interaction with CTNNB1. Interacts with TNIK. Interacts with MAD2L2; prevents TCF7L2/TCF4 binding to promZIPK/DAPK3oters, negatively modulating its transcriptional activity. Interacts with ZIPK/DAPK3. Interacts with XIAP/BIRC4 and TLE3. Interacts with DDIT3/CHOP. The CTNNB1 and TCF7L2/TCF4 complex interacts with PML (isoform PML-4). Identified in a complex with CTNNB1 and FERMT2. Interacts with SPIN1 (PubMed:22258766, PubMed:24589551, PubMed:29061846). Interacts with C11orf84/SPINDOC in a SPIN1-dependent manner (PubMed:29061846). Interacts with DAZAP2; the interaction results in localization of DAZAP2 to the nucleus (PubMed:19304756)

  • SwissProt ID

    Q9NQB0

  • Gene ID
    6934 [NCBI]
  • Synonyms

    TCF7L2; HMG box transcription factor 4; HTCF-4; T-cell factor-4 variant X2; T-cell factor 4; Transcription factor 7-like 2; TCF-4

  • Research Field

    Epigenetics and Nuclear Signaling

TCF-4/TCF7L2 Antibody (YA2167) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung cancer using TCF-4/TCF7L2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82422, 1/100) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • TCF-4/TCF7L2 Antibody (YA2167) Product Image
    1/16
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA2167)
  • TCF-4/TCF7L2 Antibody (YA2167) Product Image
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA2167)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung cancer using TCF-4/TCF7L2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82422, 1/100) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TCF-4/TCF7L2 Antibody (YA2167)
  • TCF-4/TCF7L2 Antibody (YA2167) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lung cancer using TCF-4/TCF7L2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P82422, 1/100) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • TCF-4/TCF7L2 Antibody (YA2167) Product Image IHC-P