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  4. MGST1 Antibody (YA1636)

MGST1 Antibody (YA1636) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MGST1.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay
  • Product Detail

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Description

MGST1 Antibody (YA1636) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MGST1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight
Predicted band size: 18 kDa;
Observed band size: 18 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Rat
SwissProt ID
Gene ID
Immunogen

A synthetic peptide of human Microsomal Glutathione S-transferase 1

Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image
ALL IHC-P mIHC
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using MGST1 Antibody (YA1636). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81891, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using MGST1 Antibody (YA1636). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81891, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human liver tissue using MGST1 Antibody (YA1636). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81891, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)(HY-D1835). Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human lung adenocarcinoma tissue using MGST1 Antibody (YA1636). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81891, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)(HY-D1835). Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human breast cancer tissue using MGST1 Antibody (YA1636). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81891, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed using a Bicolor signal amplification fluorescence staining kit. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human kidney tissue using MGST1 Antibody (YA1636). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81891, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed using a Bicolor signal amplification fluorescence staining kit. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

Background
Function:Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles
Subcellular Localization:Endoplasmic reticulum membrane; Multi-pass membrane protein; Mitochondrion outer membrane
Expression:
Tissue_specificity:Highly expressed in liver
Isoforms & Post-Translational Modification:P10620 has 2 isomers: P10620-1: 17599 Da (predicted); P10620-2: 9984 Da (predicted).
Subunit:Homotrimer; The trimer binds only one molecule of glutathione
RRID
Database
Research Field

Cell Biology

Synonyms
Microsomal GST-I
Documentation

MGST1 Antibody (YA1636) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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MGST1 Antibody (YA1636)
Cat. No.:
HY-P81891
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