Copy Link Print Information

NOX2 Antibody (YA6865)

(Synonyms: CYBB; NOX2; Cytochrome b-245 heavy chain; CGD91-phox; Cytochrome b; 558; subunit beta; Cytochrome b558 subunit beta; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2Neutrophil cytochrome b 91 kDa polypeptide; Superoxide-generating NADPH oxidase heavy chain subunit; gp91-1; gp91-phox; p22 phagocyte B-cytochrome)
Customer Review

Based on 1 Customer Validation

NOX2 Antibody (YA6865) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NOX2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for NOX2 Antibody (YA6865)
  • Experimental Validation Results for NOX2 Antibody (YA6865)
  • Experimental Validation Results for NOX2 Antibody (YA6865)
  • Experimental Validation Results for NOX2 Antibody (YA6865)
  • Experimental Validation Results for NOX2 Antibody (YA6865)
  • NOX2 Antibody (YA6865) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:5000 1:500 1:1000 1:500 1-2μg/sample

Product Details

Description

NOX2 Antibody (YA6865) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NOX2.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 65 kDa
Species Reactivity Database
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719278

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NOX2 Antibody (YA6865)
    Western blot analysis of extracts from RAW164.7(lane 2(20ug) and RAW164.7(lane 3(40ug) using NOX2 Antibody (HY-P87174) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for NOX2 Antibody (YA6865)
    Immunohistochemical analysis of paraffin-embedded rat liver tissue using NOX2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for NOX2 Antibody (YA6865)
    Immunohistochemical analysis of paraffin-embedded rat liver tissue using NOX2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for NOX2 Antibody (YA6865)
    Immunocytochemistry analysis of RAW 264.7 cells labeling NOX2 with NOX2 Antibody (HY-P87174)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NOX2 Antibody (HY-P87174) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for NOX2 Antibody (YA6865)
    Immunocytochemistry analysis of RAW 264.7 cells labeling NOX2 with NOX2 Antibody (HY-P87174) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NOX2 Antibody (HY-P87174) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    NOX2 (NADPH oxidase 2) is a catalytic member of the NADPH oxidase family that is specialized for reactive oxygen species (ROS) generation and serves as a central component of phagocyte antimicrobial defense and redox signaling pathways[1][2]. NOX2 activation requires assembly of membrane and cytosolic regulatory subunits, enabling superoxide production at the plasma membrane or phagosomal membrane during immune responses[2]. Mechanistically, NOX2-derived ROS contribute not only to pathogen killing but also to immune regulation, antigen presentation, and maintenance of self-tolerance, linking oxidative signaling to both innate and adaptive immunity[3][4]. Dysregulated NOX2 activity has been implicated in multiple inflammatory and oxidative stress-associated disease models, while genetic deficiency of NOX2 causes chronic granulomatous disease characterized by impaired microbial killing and recurrent infections[3][5]. In disease models, NOX2-derived ROS have been associated with inflammation, fibrosis, and cardiovascular pathology, highlighting the broad biological relevance of this isoform[5][6]. Compared with related NOX isoforms, NOX2 is predominantly expressed in phagocytic cells, whereas NOX1, NOX3, NOX4, and NOX5 are primarily expressed in non-phagocytic tissues, indicating distinct physiological functions and cellular contexts[7]. For experimental applications, NOX inhibitors and NOX2-targeted inhibitory peptides such as NOX2ds-tat have been used to investigate NOX2-dependent signaling; however, limited isoform selectivity remains an important consideration when interpreting pharmacological studies[8].

  • Subcellular Localization

    Cell membrane

  • Expression


    Tissue_Specificity: Detected in neutrophils (at protein level)

  • Isoforms & Post-Translational Modification

    P04839: 570 amino acids, molecular weight 65336 Da.

  • Subunit

    Component of the phagocyte NADPH oxidase core complex/cytochrome b558 complex, composed of CYBB (heavy chain (beta)) and CYBA (light chain (alpha)) (PubMed:3600769, PubMed:36241643, PubMed:36413210)

  • SwissProt ID

    P04839

  • Gene ID
    1536 [NCBI]
  • Synonyms

    CYBB; NOX2; Cytochrome b-245 heavy chain; CGD91-phox; Cytochrome b; 558; subunit beta; Cytochrome b558 subunit beta; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2Neutrophil cytochrome b 91 kDa polypeptide; Superoxide-generating NADPH oxidase heavy chain subunit; gp91-1; gp91-phox; p22 phagocyte B-cytochrome

[1]. Cipriano A, et al. NADPH Oxidases: From Molecular Mechanisms to Current Inhibitors. J Med Chem. 2023 Sep 14;66(17):11632-11655. [Content Brief]

[2]. Paclet MH, et al. Regulation of Neutrophil NADPH Oxidase, NOX2: A Crucial Effector in Neutrophil Phenotype and Function. Front Cell Dev Biol. 2022 Jul 14;10:945749. [Content Brief]

[3]. Altenhöfer S, et al. Evolution of NADPH Oxidase Inhibitors: Selectivity and Mechanisms for Target Engagement. Antioxid Redox Signal. 2015 Aug 10;23(5):406-27. [Content Brief]

NOX2 Antibody (YA6865) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from RAW164.7(lane 2(20ug) and RAW164.7(lane 3(40ug) using NOX2 Antibody (HY-P87174) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using NOX2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using NOX2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of RAW 264.7 cells labeling NOX2 with NOX2 Antibody (HY-P87174)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NOX2 Antibody (HY-P87174) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of RAW 264.7 cells labeling NOX2 with NOX2 Antibody (HY-P87174) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NOX2 Antibody (HY-P87174) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • NOX2 Antibody (YA6865) Product Image
    1/16
  • Experimental Validation Results for NOX2 Antibody (YA6865)
  • Experimental Validation Results for NOX2 Antibody (YA6865)
  • Experimental Validation Results for NOX2 Antibody (YA6865)
  • Experimental Validation Results for NOX2 Antibody (YA6865)
  • Experimental Validation Results for NOX2 Antibody (YA6865)
  • NOX2 Antibody (YA6865) Product Image
  • Experimental Validation Results for NOX2 Antibody (YA6865)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from RAW164.7(lane 2(20ug) and RAW164.7(lane 3(40ug) using NOX2 Antibody (HY-P87174) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for NOX2 Antibody (YA6865)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using NOX2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for NOX2 Antibody (YA6865)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using NOX2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for NOX2 Antibody (YA6865)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of RAW 264.7 cells labeling NOX2 with NOX2 Antibody (HY-P87174)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NOX2 Antibody (HY-P87174) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for NOX2 Antibody (YA6865)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of RAW 264.7 cells labeling NOX2 with NOX2 Antibody (HY-P87174) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NOX2 Antibody (HY-P87174) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for NOX2 Antibody (YA6865)
  • Experimental Validation Results for NOX2 Antibody (YA6865)
  • Experimental Validation Results for NOX2 Antibody (YA6865)
  • Experimental Validation Results for NOX2 Antibody (YA6865)
  • Experimental Validation Results for NOX2 Antibody (YA6865)
  • NOX2 Antibody (YA6865) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from RAW164.7(lane 2(20ug) and RAW164.7(lane 3(40ug) using NOX2 Antibody (HY-P87174) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • NOX2 Antibody (YA6865) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat liver tissue using NOX2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • NOX2 Antibody (YA6865) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat liver tissue using NOX2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • NOX2 Antibody (YA6865) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of RAW 264.7 cells labeling NOX2 with NOX2 Antibody (HY-P87174)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NOX2 Antibody (HY-P87174) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • NOX2 Antibody (YA6865) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of RAW 264.7 cells labeling NOX2 with NOX2 Antibody (HY-P87174) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with NOX2 Antibody (HY-P87174) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • NOX2 Antibody (YA6865) Product Image WB
  • NOX2 Antibody (YA6865) Product Image IHC-P
  • NOX2 Antibody (YA6865) Product Image IHC-P
  • NOX2 Antibody (YA6865) Product Image ICC
  • NOX2 Antibody (YA6865) Product Image ICC