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LDHD Antibody (YA6635)

(Synonyms: D lactate dehydrogenase antibody; DLD antibody; Lactate dehydrogenase D antibody; Ldhd antibody; LDHD_HUMAN antibody; MGC57726 antibody; mitochondrial antibody; Probable D lactate dehydrogenase mitochondrial antibody; Probable D-lactate dehydrogenase antibody; )
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Based on 1 Customer Validation

LDHD Antibody (YA6635) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LDHD.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for LDHD Antibody (YA6635)
  • LDHD Antibody (YA6635) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:2000 1:50-1:100 1:50-1:200

Product Details

Description

LDHD Antibody (YA6635) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LDHD.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 55 kDa
Immunogen

Recombinant protein within Human LDHD aa 368-507 / 507.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for LDHD Antibody (YA6635)
    Western blot analysis was performed on protein extracts (25 μg) from Mouse kidney (lane 2), Mouse liver (lane 3), Rat liver (lane 4), Rat kidney (lane 5), HepG2 (lane 6), and MCF-7 (lane 7) using LDHD antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    LDHD is involved in D-lactate, but not L-lactate catabolic process

  • Subcellular Localization

    Mitochondrion

  • Expression


    Tissue_Specificity: Expressed moderately in heart and liver and at lower levels in skeletal muscle and kidney

  • Isoforms & Post-Translational Modification

    Q86WU2 has two isomers: Q86WU2-1: 54871 Da (predicted); Q86WU2-2: 52144 Da (predicted).

  • Subunit

    Interacts with CSRP3

  • SwissProt ID

    Q86WU2

  • Gene ID
    197257 [NCBI]
  • Synonyms

    D lactate dehydrogenase antibody; DLD antibody; Lactate dehydrogenase D antibody; Ldhd antibody; LDHD_HUMAN antibody; MGC57726 antibody; mitochondrial antibody; Probable D lactate dehydrogenase mitochondrial antibody; Probable D-lactate dehydrogenase antibody;

LDHD Antibody (YA6635) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Mouse kidney (lane 2), Mouse liver (lane 3), Rat liver (lane 4), Rat kidney (lane 5), HepG2 (lane 6), and MCF-7 (lane 7) using LDHD antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • LDHD Antibody (YA6635) Product Image
    1/16
  • Experimental Validation Results for LDHD Antibody (YA6635)
  • LDHD Antibody (YA6635) Product Image
  • Experimental Validation Results for LDHD Antibody (YA6635)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Mouse kidney (lane 2), Mouse liver (lane 3), Rat liver (lane 4), Rat kidney (lane 5), HepG2 (lane 6), and MCF-7 (lane 7) using LDHD antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for LDHD Antibody (YA6635)
  • LDHD Antibody (YA6635) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from Mouse kidney (lane 2), Mouse liver (lane 3), Rat liver (lane 4), Rat kidney (lane 5), HepG2 (lane 6), and MCF-7 (lane 7) using LDHD antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • LDHD Antibody (YA6635) Product Image WB