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CDKN2A/p16INK4a Antibody (YA6130)

(Synonyms: Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3; Cyclin-dependent kinase 4 inhibitor A; CDK4I; Multiple tumor suppressor 1; MTS-1; p16-INK4a; p16-INK4; p16INK4A; p16; )
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Based on 1 publication(s) in Google Scholar

CDKN2A/p16INK4a Antibody (YA6130) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDKN2A/p16INK4a.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for CDKN2A/p16INK4a Antibody (YA6130)
  • Experimental Validation Results for CDKN2A/p16INK4a Antibody (YA6130)
  • Experimental Validation Results for CDKN2A/p16INK4a Antibody (YA6130)
  • CDKN2A/p16INK4a Antibody (YA6130) Product Image
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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:5000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

CDKN2A/p16INK4a Antibody (YA6130) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDKN2A/p16INK4a.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 17 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 17 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719157

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CDKN2A/p16INK4a Antibody (YA6130)
    Western blot analysis of extracts from HeLa(lane 2(20μg)), C6(lane 3(20μg)), L929(lane 4(20μg)) and MCF-7(lane 5(20μg)) using CDKN2A/p16INK4a Antibody（HY-P86438）. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CDKN2A/p16INK4a Antibody (YA6130)
    Immunocytochemistry analysis of Hela cells labeling CDKN2A with CDKN2A Antibody (HY-P86438) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CDKN2A Antibody (HY-P86438) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CDKN2A/p16INK4a Antibody (YA6130)
    Immunocytochemistry analysis of Hela cells labeling CDKN2A with CDKN2A Antibody (HY-P86438) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CDKN2A Antibody (HY-P86438) at 1/500 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    CDKN2A/p16INK4a acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Expression


    Tissue_specificity:It is widely expressed, but not detected in the brain or skeletal muscle. Isomer 3 is pancreas-specific.

  • Isoforms & Post-Translational Modification

    P42771 has 6 isomers: P42771-1: 16533 Da (predicted); P42771-2: 11215 Da (predicted); P42771-3: 12083 Da (predicted); Q8N726-1: 13903 Da (predicted); P42771-4: 17883 Da (predicted); Q8N726-2: 8731 Da (predicted).
    Phosphorylation seems to increase interaction with CDK4

  • Subunit

    Heterodimer with CDK4 or CDK6. Predominant p16 complexes contained CDK6. Interacts with CDK4 (both 'T-172'-phosphorylated and non-phosphorylated forms); the interaction inhibits cyclin D-CDK4 kinase activity. Interacts with ISCO2

  • SwissProt ID

    P42771

  • Gene ID
    1029 [NCBI]
  • Synonyms

    Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3; Cyclin-dependent kinase 4 inhibitor A; CDK4I; Multiple tumor suppressor 1; MTS-1; p16-INK4a; p16-INK4; p16INK4A; p16;

CDKN2A/p16INK4a Antibody (YA6130) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa(lane 2(20μg)), C6(lane 3(20μg)), L929(lane 4(20μg)) and MCF-7(lane 5(20μg)) using CDKN2A/p16INK4a Antibody（HY-P86438）. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling CDKN2A with CDKN2A Antibody (HY-P86438) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CDKN2A Antibody (HY-P86438) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling CDKN2A with CDKN2A Antibody (HY-P86438) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CDKN2A Antibody (HY-P86438) at 1/500 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CDKN2A/p16INK4a Antibody (YA6130) Product Image
    1/16
  • Experimental Validation Results for CDKN2A/p16INK4a Antibody (YA6130)
  • Experimental Validation Results for CDKN2A/p16INK4a Antibody (YA6130)
  • Experimental Validation Results for CDKN2A/p16INK4a Antibody (YA6130)
  • CDKN2A/p16INK4a Antibody (YA6130) Product Image
  • Experimental Validation Results for CDKN2A/p16INK4a Antibody (YA6130)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa(lane 2(20μg)), C6(lane 3(20μg)), L929(lane 4(20μg)) and MCF-7(lane 5(20μg)) using CDKN2A/p16INK4a Antibody（HY-P86438）. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CDKN2A/p16INK4a Antibody (YA6130)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling CDKN2A with CDKN2A Antibody (HY-P86438) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CDKN2A Antibody (HY-P86438) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CDKN2A/p16INK4a Antibody (YA6130)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling CDKN2A with CDKN2A Antibody (HY-P86438) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CDKN2A Antibody (HY-P86438) at 1/500 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CDKN2A/p16INK4a Antibody (YA6130)
  • Experimental Validation Results for CDKN2A/p16INK4a Antibody (YA6130)
  • Experimental Validation Results for CDKN2A/p16INK4a Antibody (YA6130)
  • CDKN2A/p16INK4a Antibody (YA6130) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa(lane 2(20μg)), C6(lane 3(20μg)), L929(lane 4(20μg)) and MCF-7(lane 5(20μg)) using CDKN2A/p16INK4a Antibody（HY-P86438）. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (Beta Actin, HY-P80993，1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001，1/10,000) was used for 1 hour at room temperature.

  • CDKN2A/p16INK4a Antibody (YA6130) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling CDKN2A with CDKN2A Antibody (HY-P86438) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CDKN2A Antibody (HY-P86438) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CDKN2A/p16INK4a Antibody (YA6130) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling CDKN2A with CDKN2A Antibody (HY-P86438) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CDKN2A Antibody (HY-P86438) at 1/500 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CDKN2A/p16INK4a Antibody (YA6130) Product Image WB
  • CDKN2A/p16INK4a Antibody (YA6130) Product Image ICC
  • CDKN2A/p16INK4a Antibody (YA6130) Product Image ICC