LAMP1 Antibody (YA9167)
(Synonyms: CD107a, Lamp1, Lamp 1)
Based on 1 Customer Validation
LAMP1 Antibody (YA9167) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to LAMP1.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, IHC-P, ICC/IF, IP, IF-Tissue, mIHC
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Reactivity :
human, mouse, rat
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Formulation:
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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mIHC
mIHC: Multiplex Immunohistochemical
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|---|---|
| Dilution Ratio | 1:500-1000 | 1:50-100 | 1:50-100 | 1:50-100 | 1:50-100 | 1-2μg per 100-500μg Total protein |
Product Details
LAMP1 Antibody (YA9167) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to LAMP1.
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Host Mouse
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Species Reactivityhuman, mouse, rat
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Calculated Molecular Weight Predicted band size: 120 kDa
A synthesized peptide derived from rat LA LAMP1.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at 2-8°C for 1 year, do not freeze.
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Shipping
Shipping with blue ice.
Verification Images
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Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded Mouse kidney tissue using LAMP1 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P89823, 1:50 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
Background
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Function
LAMP1 is a Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis. Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity (By similarity). Also plays an important role in NK-cells cytotoxicity. Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule (By similarity). In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content. Presents carbohydrate ligands to selectins (By similarity). Also implicated in tumor cell metastasis[1][2][3].
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Subcellular Localization
Lysosome membrane; Endosome membrane; Late endosome membrane; Cell membrane; Cytolytic granule membrane
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Isoforms & Post-Translational Modification
LAMP1 has an amino acid length of 406, molecular weight is 43865 Da.
O- and N-glycosylated; some of the N-glycans attached to LAMP-1 are polylactosaminoglycans -
Subunit
Interacts with ABCB9; this interaction strongly stabilizes ABCB9 and protects ABCB9 against lysosomal degradation.
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SwissProt ID
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Synonyms
CD107a, Lamp1, Lamp 1
Documentation
References
[1]. Eskelinen EL, et al. Disturbed cholesterol traffic but normal proteolytic function in LAMP-1/LAMP-2 double-deficient fibroblasts. Mol Biol Cell. 2004 Jul;15(7):3132-45. [Content Brief]
[2]. Cohnen A, et al. Surface CD107a/LAMP-1 protects natural killer cells from degranulation-associated damage. Blood. 2013 Aug 22;122(8):1411-8. [Content Brief]
[3]. Heffernan M, et al. Molecular characterization of P2B/LAMP-1, a major protein target of a metastasis-associated oligosaccharide structure. Cancer Res. 1989 Nov 1;49(21):6077-84. [Content Brief]
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