VEGFA Antibody (YA3814)

Cat. No.: HY-P84117: Data Sheet COA
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VEGFA Antibody (YA3814) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to VEGFA.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

VEGFA Antibody (YA3814) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to VEGFA.

Host

Mouse

Clonality

Monoclonal Antibody

Molecular Weight

Predicted band size: 27 kDa;

Observed band size: 25-50 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P15692

Gene ID

7422 [NCBI]

Immunogen

Purified recombinant fragment of human VEGFA (AA: 207-371) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed:35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity); Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed:34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed:17446437); Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth

Subcellular Localization：Cytoplasm; Nucleus; Secreted; Endoplasmic reticulum; Golgi apparatus; Secreted, extracellular space, extracellular matrix; Secreted; Secreted; Secreted

Expression：
Tissue_specificity:Higher expression in pituitary tumors than in the pituitary gland; widespread expression; widespread expression; widespread expression; not widely expressed; not widely expressed

Induction:By hypoxia. Regulated by growth factors, cytokines, gonadotropins, nitric oxide, hypoglycemia and oncogenic mutations

Isoforms & Post-Translational Modification：P15692 has 17 isomers: P15692-13: 43597 Da (predicted); P15692-1: 27042 Da (predicted); P15692-2: 25173 Da (predicted); P15692-3: 24422 Da (predicted); P15692-4: 22314 Da (predicted); P15692-5: 20218 Da (predicted); P15692-6: 20064 Da (predicted); P15692-8: 22259 Da (predicted); P15692-9: 17219 Da (predicted); P15692-10: 15981 Da (predicted); P15692-11: 40738 Da (predicted); P15692-12: 35643 Da (predicted); P15692-14: 45467 Da (predicted); P15692-15: 40683 Da (predicted); P15692-16: 42846 Da (predicted); P15692-17: 38642 Da (predicted); P15692-18: 34406 Da (predicted).
Produced by use of an alternative upstream CUG codon and post-translationally processed into the N-terminal N-VEGF form and the C-terminal secreted VEGFA form

Subunit：Homodimer; disulfide-linked (By similarity). Interacts with NRP1 (PubMed:26503042). Interacts with isoform 2 of BSG (PubMed:25825981)

RRID

AB_3718839

Synonyms

VPF; VEGF; MVCD1

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)