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VEGFA Antibody (YA3814)

(Synonyms: VPF; VEGF; MVCD1)
Customer Review

Based on 1 Customer Validation

VEGFA Antibody (YA3814) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to VEGFA.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, ICC/IF, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for VEGFA Antibody (YA3814)
  • Experimental Validation Results for VEGFA Antibody (YA3814)
  • VEGFA Antibody (YA3814) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:10000

Product Details

Description

VEGFA Antibody (YA3814) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to VEGFA.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 25-50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 27 kDa
Immunogen

Purified recombinant fragment of human VEGFA (AA: 207-371) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718839

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for VEGFA Antibody (YA3814)
    Immunocytochemistry analysis of NIH-3T3 cells labeling VEGFA with VEGFA Antibody (HY-P84117) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with VEGFA Antibody (HY-P84117) at 1/300 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for VEGFA Antibody (YA3814)
    Immunocytochemistry analysis of NIH-3T3 cells labeling VEGFA with VEGFA Antibody (HY-P84117) at 1/600 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with VEGFA Antibody (HY-P84117) at 1/600 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    VEGFA participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A. Involved in protecting cells from hypoxia-mediated cell death; Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Also binds the DEAR/FBXW7-AS1 receptor; Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth[1][2][3].

  • Subcellular Localization

    Cytoplasm; Nucleus; Secreted; Endoplasmic reticulum; Golgi apparatus; Secreted, extracellular space, extracellular matrix; Secreted; Secreted; Secreted

  • Expression


    Tissue_specificity:Higher expression in pituitary tumors than in the pituitary gland; widespread expression; widespread expression; widespread expression; not widely expressed; not widely expressed

    Induction:By hypoxia. Regulated by growth factors, cytokines, gonadotropins, nitric oxide, hypoglycemia and oncogenic mutations

  • Isoforms & Post-Translational Modification

    P15692 has 17 isomers: P15692-13: 43597 Da (predicted); P15692-1: 27042 Da (predicted); P15692-2: 25173 Da (predicted); P15692-3: 24422 Da (predicted); P15692-4: 22314 Da (predicted); P15692-5: 20218 Da (predicted); P15692-6: 20064 Da (predicted); P15692-8: 22259 Da (predicted); P15692-9: 17219 Da (predicted); P15692-10: 15981 Da (predicted); P15692-11: 40738 Da (predicted); P15692-12: 35643 Da (predicted); P15692-14: 45467 Da (predicted); P15692-15: 40683 Da (predicted); P15692-16: 42846 Da (predicted); P15692-17: 38642 Da (predicted); P15692-18: 34406 Da (predicted).
    Produced by use of an alternative upstream CUG codon and post-translationally processed into the N-terminal N-VEGF form and the C-terminal secreted VEGFA form

  • Subunit

    Homodimer; disulfide-linked (By similarity). Interacts with NRP1 (PubMed:26503042). Interacts with isoform 2 of BSG (PubMed:25825981)

  • SwissProt ID

    P15692

  • Gene ID
    7422 [NCBI]
  • Synonyms

    VPF; VEGF; MVCD1

VEGFA Antibody (YA3814) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of NIH-3T3 cells labeling VEGFA with VEGFA Antibody (HY-P84117) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with VEGFA Antibody (HY-P84117) at 1/300 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of NIH-3T3 cells labeling VEGFA with VEGFA Antibody (HY-P84117) at 1/600 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with VEGFA Antibody (HY-P84117) at 1/600 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • VEGFA Antibody (YA3814) Product Image
    1/16
  • Experimental Validation Results for VEGFA Antibody (YA3814)
  • Experimental Validation Results for VEGFA Antibody (YA3814)
  • VEGFA Antibody (YA3814) Product Image
  • Experimental Validation Results for VEGFA Antibody (YA3814)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of NIH-3T3 cells labeling VEGFA with VEGFA Antibody (HY-P84117) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with VEGFA Antibody (HY-P84117) at 1/300 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for VEGFA Antibody (YA3814)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of NIH-3T3 cells labeling VEGFA with VEGFA Antibody (HY-P84117) at 1/600 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with VEGFA Antibody (HY-P84117) at 1/600 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for VEGFA Antibody (YA3814)
  • Experimental Validation Results for VEGFA Antibody (YA3814)
  • VEGFA Antibody (YA3814) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of NIH-3T3 cells labeling VEGFA with VEGFA Antibody (HY-P84117) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with VEGFA Antibody (HY-P84117) at 1/300 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • VEGFA Antibody (YA3814) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of NIH-3T3 cells labeling VEGFA with VEGFA Antibody (HY-P84117) at 1/600 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with VEGFA Antibody (HY-P84117) at 1/600 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • VEGFA Antibody (YA3814) Product Image ICC
  • VEGFA Antibody (YA3814) Product Image ICC