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CCR2 Antibody (YA548)

(Synonyms: C C chemokine receptor type 2 antibody; C C CKR 2 antibody; C-C chemokine receptor type 2 antibody; C-C CKR-2 antibody; CC chemokine receptor type 2 antibody; CC CKR 2 antibody; CC-CKR-2 antibody; CCCKR2 antibody; CCR 2 antibody; CCR-2 antibody; CCR1L antibody; CCR2 antibody; CCR2_HUMAN antibody; CCR2A antibody; CCR2B antibody; CCR5L antibody; CD192 antibody; CD192 antigen antibody; Chemokine C C motif receptor 2 antibody; Chemokine CC Motif Receptor 2 antibody; CKR 2 antibody; CKR2 antibody; CKR2A antibody; CKR2B antibody; CMKBR2 antibody; MCP 1 R antibody; MCP-1-R antibody; MCP1 RECEPTOR antibody; MCP1R antibody; Monocyte chemoattractant protein 1 receptor antibody; Monocyte Chemotactic Protein 1 Receptor antibody; Monocyte Chemotactic Protein 1 Receptor antibody)
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Based on 1 publication(s) in Google Scholar

CCR2 Antibody (YA548) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CCR2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP, FC

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CCR2 Antibody (YA548)
  • CCR2 Antibody (YA548) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:50-1:100 Use at an assay dependent concentration.

Product Details

Description

CCR2 Antibody (YA548) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CCR2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 42 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 42 kDa
Species Reactivity Database

Entrez Gene: 729230 Human

SwissProt: P41597 Human

Immunogen

Synthetic peptide within N-terminal human CCR2.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102472

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CCR2 Antibody (YA548)
    Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat(lane 3(20μg), 293T(lane 4(20μg), K562(lane 5(20μg) using CCR2 Antibody (HY-P80385). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Key functional receptor for CCL2 but can also bind CCL7, and CCL12 (PubMed:23408426, PubMed:38157855, PubMed:8048929, PubMed:8146186). Also transduces signaling mediated by CCL13 (PubMed:38157855). Its binding with CCL2 on monocytes and macrophages mediates chemotaxis and migration induction through the activation of the PI3K cascade, the small G protein Rac and lamellipodium protrusion (PubMed:38157855). Also acts as a receptor for the beta-defensin DEFB106A/DEFB106B (PubMed:23938203). Regulates the expression of T-cell inflammatory cytokines and T-cell differentiation, promoting the differentiation of T-cells into T-helper 17 cells (Th17) during inflammation (By similarity). Facilitates the export of mature thymocytes by enhancing directional movement of thymocytes to sphingosine-1-phosphate stimulation and up-regulation of S1P1R expression; signals through the JAK-STAT pathway to regulate FOXO1 activity leading to an increased expression of S1P1R (By similarity). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (By similarity). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (By similarity). Mediates the recruitment of macrophages and monocytes to the injury site following brain injury (By similarity); (Microbial infection) Alternative coreceptor with CD4 for HIV-1 infection

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Expression by monocytes and IL2-activated NK cells (PubMed:9058802) . Highly expressed on CD14+/CD16- monocytes, and weakly expressed on CD14+/CD16+ monocytes, type 2 dendritic cells (DCs) , and plasmacytoid dendritic cells (protein level) (PubMed:38157855) .

    Induction:Up-regulated by CREB3 (PubMed:18587271) . In NK cells, induced by IL2 (PubMed:9058802)

  • Subunit

    Interacts with ARRB1 (PubMed:9501202). Interacts (via extracellular N-terminal region) with beta-defensin DEFB106A/DEFB106B; this interaction may preferentially require specific tyrosine sulfation on CCR2 (PubMed:23938203). Interacts with NUP85; the interaction is required for CCR2 clusters formation on the cell membrane and CCR2 signaling (PubMed:15995708, PubMed:25283965)

  • SwissProt ID

    P41597

  • Gene ID
    729230 [NCBI]
  • Synonyms

    C C chemokine receptor type 2 antibody; C C CKR 2 antibody; C-C chemokine receptor type 2 antibody; C-C CKR-2 antibody; CC chemokine receptor type 2 antibody; CC CKR 2 antibody; CC-CKR-2 antibody; CCCKR2 antibody; CCR 2 antibody; CCR-2 antibody; CCR1L antibody; CCR2 antibody; CCR2_HUMAN antibody; CCR2A antibody; CCR2B antibody; CCR5L antibody; CD192 antibody; CD192 antigen antibody; Chemokine C C motif receptor 2 antibody; Chemokine CC Motif Receptor 2 antibody; CKR 2 antibody; CKR2 antibody; CKR2A antibody; CKR2B antibody; CMKBR2 antibody; MCP 1 R antibody; MCP-1-R antibody; MCP1 RECEPTOR antibody; MCP1R antibody; Monocyte chemoattractant protein 1 receptor antibody; Monocyte Chemotactic Protein 1 Receptor antibody; Monocyte Chemotactic Protein 1 Receptor antibody

  • Research Field

    Immunology

CCR2 Antibody (YA548) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat(lane 3(20μg), 293T(lane 4(20μg), K562(lane 5(20μg) using CCR2 Antibody (HY-P80385). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • CCR2 Antibody (YA548) Product Image
    1/16
  • Experimental Validation Results for CCR2 Antibody (YA548)
  • CCR2 Antibody (YA548) Product Image
  • Experimental Validation Results for CCR2 Antibody (YA548)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat(lane 3(20μg), 293T(lane 4(20μg), K562(lane 5(20μg) using CCR2 Antibody (HY-P80385). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CCR2 Antibody (YA548)
  • CCR2 Antibody (YA548) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat(lane 3(20μg), 293T(lane 4(20μg), K562(lane 5(20μg) using CCR2 Antibody (HY-P80385). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • CCR2 Antibody (YA548) Product Image WB