RIT1 Antibody
(Synonyms: RIBB, RIT, ROC1, RIT1, GTP-binding protein Rit1, Ras-like protein expressed in many tissues, Ras-like without CAAX protein 1)RIT1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to RIT1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-200 |
Product Details
RIT1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to RIT1.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 25 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 25 kDa
Synthetic peptide corresponding to the C-term region of human RIT1.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
RIT1 plays a crucial role in coupling nerve growth factor (NGF) stimulation to the activation of both EPHB2 and MAPK14 signaling pathways and in NGF-dependent neuronal differentiation. Involved in ELK1 transactivation through the Ras-MAPK signaling cascade that mediates a wide variety of cellular functions, including cell proliferation, survival, and differentiation. Recognized by LZTR1, which mediates its ubiquitination by a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex[1][2][3].
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Subcellular Localization
Cell membrane
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Expression
Tissue_Specificity: Expressed in many tissues. -
Isoforms & Post-Translational Modification
RIT1 has 3 isoforms, Q92963-1: amino acid length is 219, molecular weight is 25145 Da (predicted); Q92963-2: amino acid length is 183, molecular weight is 21633 Da (predicted); Q92963-3: amino acid length is 236, molecular weight is 27148 Da (predicted).RIT1 存在 3 个异构体，Q92963-1：氨基酸个数为 219 个，分子量为 25145 Da (预测)；Q92963-2：氨基酸个数为 183 个，分子量为 21633 Da (预测)；Q92963-3：氨基酸个数为 236 个，分子量为 27148 Da (预测)。
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Subunit
Interacts with AFDN, the C-terminal domain of RALGDS and RLF, but not with RIN1 and PIK3CA.
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SwissProt ID
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Synonyms
RIBB, RIT, ROC1, RIT1, GTP-binding protein Rit1, Ras-like protein expressed in many tissues, Ras-like without CAAX protein 1
Documentation
[1]. Shi GX, et al. Rit contributes to nerve growth factor-induced neuronal differentiation via activation of B-Raf-extracellular signal-regulated kinase and p38 mitogen-activated protein kinase cascades. Mol Cell Biol. 2005 Jan;25(2):830-46. [Content Brief]
[2]. Aoki Y, et al. Gain-of-function mutations in RIT1 cause Noonan syndrome, a RAS/MAPK pathway syndrome. Am J Hum Genet. 2013 Jul 11;93(1):173-80. [Content Brief]
[3]. Dharmaiah S, et al. Structural basis for LZTR1 recognition of RAS GTPases for degradation. Science. 2025 Sep 11;389(6765):1112-1117. [Content Brief]
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