WIPI2 Antibody
(Synonyms: CGI-50, WIPI2, WD repeat domain phosphoinositide-interacting protein 2, WIPI-2, WIPI49-like protein 2)WIPI2 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to WIPI2.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-200 |
Product Details
WIPI2 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to WIPI2.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 49 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 49 kDa
Synthetic peptide corresponding to the C-term region of human WIPI2.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
WIPI2 is a Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Involved in an early step of the formation of preautophagosomal structures. Binds and is activated by phosphatidylinositol 3-phosphate (PtdIns3P) forming on membranes of the endoplasmic reticulum upon activation of the upstream ULK1 and PI3 kinases. Mediates ER-isolation membranes contacts by interacting with the ULK1:RB1CC1 complex and PtdIns3P. Once activated, WIPI2 recruits at phagophore assembly sites the ATG12-ATG5-ATG16L1 complex that directly controls the elongation of the nascent autophagosomal membrane[1][2][3].
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Subcellular Localization
Preautophagosomal structure membrane
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Expression
Tissue_Specificity: Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas. Expression is down-regulated in pancreatic and in kidney tumors.
Induction: Expression is repressed by ZKSCAN3. -
Isoforms & Post-Translational Modification
WIPI2 has 6 isoforms, Q9Y4P8-1: amino acid length is 454, molecular weight is 49408 Da (predicted); Q9Y4P8-2: amino acid length is 425, molecular weight is 46384 Da (predicted); Q9Y4P8-3: amino acid length is 384, molecular weight is 42077 Da (predicted); Q9Y4P8-4: amino acid length is 436, molecular weight is 47543 Da (predicted); Q9Y4P8-5: amino acid length is 438, molecular weight is 48251 Da (predicted); Q9Y4P8-6: amino acid length is 443, molecular weight is 48249 Da (predicted).
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Subunit
Interacts with TECPR1.
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SwissProt ID
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Synonyms
CGI-50, WIPI2, WD repeat domain phosphoinositide-interacting protein 2, WIPI-2, WIPI49-like protein 2
Documentation
[1]. Polson HE, et al. Mammalian Atg18 (WIPI2) localizes to omegasome-anchored phagophores and positively regulates LC3 lipidation. Autophagy. 2010 May;6(4):506-22. [Content Brief]
[2]. Bakula D, et al. WIPI3 and WIPI4 β-propellers are scaffolds for LKB1-AMPK-TSC signalling circuits in the control of autophagy. Nat Commun. 2017 May 31;8:15637. [Content Brief]
[3]. Zhao YG, et al. The ER-Localized Transmembrane Protein EPG-3/VMP1 Regulates SERCA Activity to Control ER-Isolation Membrane Contacts for Autophagosome Formation. Mol Cell. 2017 Sep 21;67(6):974-989.e6. [Content Brief]
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