AEBP2 Antibody (YA4347)(PBS only)

Cat. No.: HY-P84650A: Data Sheet User Guide for Antibodies
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AEBP2 Antibody (YA4347) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to AEBP2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

AEBP2 Antibody (YA4347) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to AEBP2.

Host

Mouse

Molecular Weight

Predicted band size: 54 kDa;

Observed band size: 55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q6ZN18

Gene ID

121536 [NCBI]

Immunogen

Purified recombinant fragment of human AEBP2 (AA: 358-495) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-1:500
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Solution

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Acts as an accessory subunit for the core Polycomb repressive complex 2 (PRC2), which mediates histone H3K27 (H3K27me3) trimethylation on chromatin leading to transcriptional repression of the affected target gene (PubMed:15225548, PubMed:29499137, PubMed:31959557). Plays a role in nucleosome localization of the PRC2 complex (PubMed:29499137)

Subcellular Localization：Nucleus

Subunit：Self-associates (PubMed:15225548). Associates with the PRC2 complex, which consists of the core components EED, EZH1 or EZH2, SUZ12, and RBBP4, and various combinations of accessory subunits including AEBP2, JARID2, PHF19, MTF2 and EPOP (PubMed:12351676, PubMed:15225548, PubMed:29499137, PubMed:31959557). Found in a monomeric PRC2.2 (class 2) complex consisting of at least SUZ12, RBBP4, AEBP2 and JARID2 (PubMed:29499137). Within the PRC2 complex, interacts directly with SUZ12; competes with PHF19 for SUZ12 binding (PubMed:12351676, PubMed:29499137). Interacts with EED, EZH2, and RBBP4 (PubMed:12351676, PubMed:15225548). May also interact with RBBP7 (PubMed:12351676)

Synonyms

Adipocyte enhancer-binding protein 2, AE-binding protein 2, AEBP 2

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/hcc1954-cell.html