Matriptase-2 Antibody (YA8957)
(Synonyms: UNQ354/PRO618, TMPRSS6, Transmembrane protease serine 6, Matriptase-2)
Based on 1 Customer Validation
Matriptase-2 Antibody (YA8957) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Matriptase-2.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, ICC/IF, IF-Tissue, IP, ELISA
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Reactivity :
human
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Formulation:
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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IP
IP: Immunoprecipitation
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|---|
| Dilution Ratio | 1:100-1000 | 1:50-500 | 1:50-500 | 1-2μg per 100-500μg Total protein | 1:30-3000 |
Product Details
Matriptase-2 Antibody (YA8957) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Matriptase-2.
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Host Mouse
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Species Reactivityhuman
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Observed Molecular WeightObserved band size: 70 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 89 kDa
OMIM: 609862
A synthesized peptide derived from human Matriptase-2.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at 2-8°C for 1 year, do not freeze.
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Shipping
Shipping with blue ice.
Background
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Function
Matriptase-2 is a Membrane-bound serine protease. Through the cleavage of cell surface hemojuvelin (HJV), a regulator of the expression of the iron absorption-regulating hormone hepicidin/HAMP, plays a role in iron homeostasis[1][2][3][4][5].
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Subcellular Localization
Cell membrane
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Isoforms & Post-Translational Modification
Matriptase-2 has 4 isoforms, Q8IU80-4: amino acid length is 811, molecular weight is 90000 Da (predicted); Q8IU80-1: amino acid length is 802, molecular weight is 88874 Da (predicted); Q8IU80-2: amino acid length is 461, molecular weight is 51602 Da (predicted); Q8IU80-5: amino acid length is 824, molecular weight is 91333 Da (predicted).
The single-chain zymogen undergoes autoproteolytic processing. This results in TMPRSS6 shedding from the cell surface and conversion into an activated two-chains form which is released extracellularly. The process involves a trans-activation mechanism that requires TMPRSS6 oligomerization -
Subunit
Interacts with HJV
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SwissProt ID
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Synonyms
UNQ354/PRO618, TMPRSS6, Transmembrane protease serine 6, Matriptase-2
Documentation
[1]. Silvestri L, et al. The serine protease matriptase-2 (TMPRSS6) inhibits hepcidin activation by cleaving membrane hemojuvelin. Cell Metab. 2008 Dec;8(6):502-11. [Content Brief]
[2]. Stirnberg M, et al. Proteolytic processing of the serine protease matriptase-2: identification of the cleavage sites required for its autocatalytic release from the cell surface. Biochem J. 2010 Aug 15;430(1):87-95. [Content Brief]
[3]. De Falco L, et al. Functional and clinical impact of novel TMPRSS6 variants in iron-refractory iron-deficiency anemia patients and genotype-phenotype studies. Hum Mutat. 2014 Nov;35(11):1321-9. [Content Brief]
[4]. McDonald CJ, et al. Functional analysis of matriptase-2 mutations and domains: insights into the molecular basis of iron-refractory iron deficiency anemia. Am J Physiol Cell Physiol. 2015 Apr 1;308(7):C539-47. [Content Brief]
[5]. Finberg KE, et al. Mutations in TMPRSS6 cause iron-refractory iron deficiency anemia (IRIDA). Nat Genet. 2008 May;40(5):569-71. [Content Brief]
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