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CD9 Antibody (YA524)

Customer Review

Based on 1 Customer Validation

CD9 Antibody (YA524) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD9.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, IP

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for CD9 Antibody (YA524)
  • Experimental Validation Results for CD9 Antibody (YA524)
  • Experimental Validation Results for CD9 Antibody (YA524)
  • Experimental Validation Results for CD9 Antibody (YA524)
  • Experimental Validation Results for CD9 Antibody (YA524)
  • Experimental Validation Results for CD9 Antibody (YA524)
  • Experimental Validation Results for CD9 Antibody (YA524)
  • Experimental Validation Results for CD9 Antibody (YA524)
  • CD9 Antibody (YA524) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500 1:50 1:50-1:200 Use at an assay dependent concentration.

Product Details

Description

CD9 Antibody (YA524) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD9.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 23 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 25 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human CD9 AA. range:179-228.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102066

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD9 Antibody (YA524)
    Western blot analysis of extracts from Hela(lane 1(20μg)) 、HCT116(lane 2(20μg)) 、​Mouse liver(lane 3(20μg))and Mouse lung(lane 4(20μg) using CD9 Antibody（HY-P80068）. Proteins were transferred to a PVDF membrane and blocked with 5%BSA in TBST for 1.5 hour at room temperature. The primary antibody ( 1/500) and Loading control antibody (GAPDH, 1/1000) was used in 5% BSA in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD9 Antibody (YA524)
    Western blot analysis of extracts from K562(lane 2(20ug) , MCF-7(lane 3(20ug) and HCT116(lane 4(20ug) using CD43 Antibody (HY-P80068) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD9 Antibody (YA524)
    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using CD9 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD9 Antibody (YA524)
    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using CD9 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD9 Antibody (YA524)
    Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue using CD9 Antibody (YA524). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80068, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for CD9 Antibody (YA524)
    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using CD9 Antibody (YA524). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80068, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for CD9 Antibody (YA524)
    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using CD9 Antibody (YA524). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80068, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for CD9 Antibody (YA524)
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CD9 Antibody (YA524). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80068, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

Background

  • Function

    Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion (PubMed:14575715, PubMed:18541721, PubMed:8478605). Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion (By similarity). In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration (By similarity). In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles (PubMed:12796480). Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption (By similarity). Acts as a receptor for PSG17 (By similarity). Involved in platelet activation and aggregation (PubMed:18541721). Regulates paranodal junction formation (By similarity). Involved in cell adhesion, cell motility and tumor metastasis (PubMed:7511626, PubMed:8478605)

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein; Membrane; Multi-pass membrane protein; Secreted, extracellular exosome

  • Expression


    Tissue_specificity:Protein levels were detected in platelets (PubMed: 19640571) . It is expressed by various hematopoietic cells and epithelial cells (PubMed: 19640571) .

  • Subunit

    Forms both disulfide-linked homodimers and higher homooligomers as well as heterooligomers with other members of the tetraspanin family (PubMed:14556650). Interacts (via the second extracellular domain) with integrin ITGAV:ITGB3 (PubMed:19640571, PubMed:27993971). Interacts with integrin ITGA6:ITGB1; interaction takes place in oocytes and is involved in sperm-egg fusion (By similarity). Part of integrin-tetraspanin complexes composed of CD81, beta-1 and beta-2 integrins in the membrane of monocyte/macrophages (PubMed:12796480). Interacts with CD63; identified in a complex with CD63 and ITGB3 (PubMed:19640571). Associates with CR2/CD21 and with PTGFRN/CD9P1 (PubMed:11278880). Part of a complex composed of CD9, CD81, PTGFRN and IGSF8 (By similarity). Interacts directly with IGSF8 (PubMed:11504738). Interacts with PDPN; this interaction is homophilic and attenuates platelet aggregation and pulmonary metastasis induced by PDPN (PubMed:18541721). Interacts (on T cell side) with CD81 at immunological synapses between antigen-presenting cells and T cells (PubMed:23858057)

  • SwissProt ID

    P21926

  • Gene ID
    928 [NCBI]
  • Research Field

    Immunology

CD9 Antibody (YA524) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 1(20μg)) 、HCT116(lane 2(20μg)) 、​Mouse liver(lane 3(20μg))and Mouse lung(lane 4(20μg) using CD9 Antibody（HY-P80068）. Proteins were transferred to a PVDF membrane and blocked with 5%BSA in TBST for 1.5 hour at room temperature. The primary antibody ( 1/500) and Loading control antibody (GAPDH, 1/1000) was used in 5% BSA in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20ug) , MCF-7(lane 3(20ug) and HCT116(lane 4(20ug) using CD43 Antibody (HY-P80068) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using CD9 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using CD9 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue using CD9 Antibody (YA524). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80068, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using CD9 Antibody (YA524). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80068, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using CD9 Antibody (YA524). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80068, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CD9 Antibody (YA524). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80068, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • CD9 Antibody (YA524) Product Image
    1/16
  • Experimental Validation Results for CD9 Antibody (YA524)
  • Experimental Validation Results for CD9 Antibody (YA524)
  • Experimental Validation Results for CD9 Antibody (YA524)
  • Experimental Validation Results for CD9 Antibody (YA524)
  • Experimental Validation Results for CD9 Antibody (YA524)
  • Experimental Validation Results for CD9 Antibody (YA524)
  • Experimental Validation Results for CD9 Antibody (YA524)
  • Experimental Validation Results for CD9 Antibody (YA524)
  • CD9 Antibody (YA524) Product Image
  • Experimental Validation Results for CD9 Antibody (YA524)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 1(20μg)) 、HCT116(lane 2(20μg)) 、​Mouse liver(lane 3(20μg))and Mouse lung(lane 4(20μg) using CD9 Antibody（HY-P80068）. Proteins were transferred to a PVDF membrane and blocked with 5%BSA in TBST for 1.5 hour at room temperature. The primary antibody ( 1/500) and Loading control antibody (GAPDH, 1/1000) was used in 5% BSA in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD9 Antibody (YA524)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20ug) , MCF-7(lane 3(20ug) and HCT116(lane 4(20ug) using CD43 Antibody (HY-P80068) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD9 Antibody (YA524)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using CD9 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD9 Antibody (YA524)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using CD9 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD9 Antibody (YA524)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue using CD9 Antibody (YA524). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80068, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for CD9 Antibody (YA524)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using CD9 Antibody (YA524). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80068, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for CD9 Antibody (YA524)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using CD9 Antibody (YA524). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80068, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for CD9 Antibody (YA524)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CD9 Antibody (YA524). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80068, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for CD9 Antibody (YA524)
  • Experimental Validation Results for CD9 Antibody (YA524)
  • Experimental Validation Results for CD9 Antibody (YA524)
  • Experimental Validation Results for CD9 Antibody (YA524)
  • Experimental Validation Results for CD9 Antibody (YA524)
  • Experimental Validation Results for CD9 Antibody (YA524)
  • Experimental Validation Results for CD9 Antibody (YA524)
  • Experimental Validation Results for CD9 Antibody (YA524)
  • CD9 Antibody (YA524) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela(lane 1(20μg)) 、HCT116(lane 2(20μg)) 、​Mouse liver(lane 3(20μg))and Mouse lung(lane 4(20μg) using CD9 Antibody（HY-P80068）. Proteins were transferred to a PVDF membrane and blocked with 5%BSA in TBST for 1.5 hour at room temperature. The primary antibody ( 1/500) and Loading control antibody (GAPDH, 1/1000) was used in 5% BSA in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • CD9 Antibody (YA524) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from K562(lane 2(20ug) , MCF-7(lane 3(20ug) and HCT116(lane 4(20ug) using CD43 Antibody (HY-P80068) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • CD9 Antibody (YA524) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using CD9 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD9 Antibody (YA524) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using CD9 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD9 Antibody (YA524) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue using CD9 Antibody (YA524). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80068, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • CD9 Antibody (YA524) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using CD9 Antibody (YA524). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80068, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • CD9 Antibody (YA524) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using CD9 Antibody (YA524). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80068, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • CD9 Antibody (YA524) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CD9 Antibody (YA524). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80068, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • CD9 Antibody (YA524) Product Image WB
  • CD9 Antibody (YA524) Product Image WB
  • CD9 Antibody (YA524) Product Image IHC-P
  • CD9 Antibody (YA524) Product Image IHC-P
  • CD9 Antibody (YA524) Product Image IHC-P
  • CD9 Antibody (YA524) Product Image IHC-P
  • CD9 Antibody (YA524) Product Image IHC-P
  • CD9 Antibody (YA524) Product Image IHC-P