BANP Antibody (YA10071)
(Synonyms: BEND1, SMAR1, BANP, Protein BANP, BEN domain-containing protein 1, Btg3-associated nuclear protein, Scaffold/matrix-associated region-1-binding protein)BANP Antibody (YA10071) is a Mouse-derived and non-conjugated IgG1 Monoclonal antibody, targeting to BANP.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
BANP Antibody (YA10071) is a Mouse-derived and non-conjugated IgG1 Monoclonal antibody, targeting to BANP.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 70 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 56 kDa
Purified recombinant protein of human BANP.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
BANP controls V(D)J recombination during T-cell development by repressing T-cell receptor (TCR) beta enhancer function (By similarity). Binds to scaffold/matrix attachment region beta (S/MARbeta), an ATC-rich DNA sequence located upstream of the TCR beta enhancer (By similarity). Represses cyclin D1 transcription by recruiting HDAC1 to its promoter, thereby diminishing H3K9ac, H3S10ph and H4K8ac levels. Promotes TP53 activation, which causes cell cycle arrest (By similarity). Plays a role in the regulation of alternative splicing. Binds to CD44 pre-mRNA and negatively regulates the inclusion of CD44 proximal variable exons v2-v6 but has no effect on distal variable exons v7-v10[1][2].
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Subcellular Localization
Nucleus; Nucleus speckle; Cytoplasm
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Expression
Tissue_Specificity: Down-regulated in breast cancer cell lines. -
Isoforms & Post-Translational Modification
BANP has 7 isoforms, Q8N9N5-1: amino acid length is 519, molecular weight is 56494 Da (predicted); Q8N9N5-2: amino acid length is 469, molecular weight is 51243 Da (predicted); Q8N9N5-3: amino acid length is 442, molecular weight is 48404 Da (predicted); Q8N9N5-4: amino acid length is 491, molecular weight is 53527 Da (predicted); Q8N9N5-5: amino acid length is 497, molecular weight is 54243 Da (predicted); Q8N9N5-6: amino acid length is 466, molecular weight is 50905 Da (predicted); Q8N9N5-7: amino acid length is 508, molecular weight is 55502 Da (predicted).
MAPK1/MAPK3-mediated phosphorylation at Thr-337 and Thr-352 results in export to the cytoplasm. -
Subunit
Part of a corepressor complex containing BANP, HDAC1, SIN3A, SIN3B, RBL1 and RBL2.
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SwissProt ID
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Synonyms
BEND1, SMAR1, BANP, Protein BANP, BEN domain-containing protein 1, Btg3-associated nuclear protein, Scaffold/matrix-associated region-1-binding protein
Documentation
References
[1]. Rampalli S, et al. Tumor suppressor SMAR1 mediates cyclin D1 repression by recruitment of the SIN3/histone deacetylase 1 complex. Mol Cell Biol. 2005 Oct;25(19):8415-29. [Content Brief]
[2]. Nakka KK, et al. Nuclear matrix-associated protein SMAR1 regulates alternative splicing via HDAC6-mediated deacetylation of Sam68. Proc Natl Acad Sci U S A. 2015 Jun 30;112(26):E3374-83. [Content Brief]
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