BCAR1 Antibody (YA2560)(PBS only)

Cat. No.: HY-P82815A: Data Sheet User Guide for Antibodies
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BCAR1 Antibody (YA2560) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BCAR1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

BCAR1 Antibody (YA2560) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BCAR1.

Host

Rabbit

Molecular Weight

Predicted band size: 93 kDa;

Observed band size: 130 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P56945

Gene ID

9564 [NCBI]

Immunogen

A synthesized peptide derived from human BCAR1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion (PubMed:12432078, PubMed:12832404). Implicated in induction of cell migration and cell branching (PubMed:12432078, PubMed:12832404, PubMed:17038317). Involved in the BCAR3-mediated inhibition of TGFB signaling (By similarity)

Subcellular Localization：Cell junction, focal adhesion; Cytoplasm; Cell projection, axon

Expression：
Tissue_specificity:Expressed in B-cells (at protein level) (PubMed:9020138) . Widely expressed with an abundant expression in the testis (PubMed:10639512) . Low level of expression seen in the liver, thymus, and peripheral blood leukocytes (PubMed:10639512)

Isoforms & Post-Translational Modification：P56945 has 8 isomers: P56945-1: 93372 Da (predicted); P56945-2: 95469 Da (predicted); P56945-3: 95454 Da (predicted); P56945-4: 77731 Da (predicted); P56945-5: 93137 Da (predicted); P56945-6: 97877 Da (predicted); P56945-7: 95216 Da (predicted); P56945-8: 93291 Da (predicted).
PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix;Dephosphorylated by PTPN14 at Tyr-128;Phosphorylated by SRC kinase in a EDN1- and PTK2B-mediated manner; phosphorylation strengthens its interaction with BCAR3 as part of the PTK2B/BCAR1/BCAR3/RAP1 signaling pathway

Subunit：Forms complexes in vivo with PTK2/FAK1, adapter protein CRKL and LYN kinase (PubMed:9020138). Heterodimerizes with NEDD9 (PubMed:10502414). Component of a complex comprised of SH2D3C, BCAR1/CAS, and CRK (PubMed:12432078).

Research Field

Signal Transduction

Synonyms

BCAR 1; CAS; CAS1; Cass1; CRKAS; p130cas

Documentation

User Guide for Antibodies (1077 KB)