MetRS Antibody (YA3196)(PBS only)

Cat. No.: HY-P83451A: Data Sheet User Guide for Antibodies
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MetRS Antibody (YA3196) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MetRS.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

MetRS Antibody (YA3196) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MetRS.

Host

Rabbit

Molecular Weight

Predicted band size: 101 kDa;

Observed band size: 101 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

P56192

Gene ID

4141 [NCBI]

Immunogen

A synthetic peptide of human MARS

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:500-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
FC FC: Flow Cytometry	1:20-1:50
IP IP: Immunoprecipitation	1:20-1:50

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed:11714285). Plays a role in the synthesis of ribosomal RNA in the nucleolus (PubMed:10791971)

Subcellular Localization：Cytoplasm, cytosol; Nucleus, nucleolus

Isoforms & Post-Translational Modification：P56192 has 2 isomers: P56192-1: 101116 Da (predicted); P56192-2: 61816 Da (predicted).

Subunit：Monomer (PubMed:11714285). Part of a multisubunit complex that groups tRNA ligases for Arg (RARS1), Asp (DARS1), Gln (QARS1), Ile (IARS1), Leu (LARS1), Lys (KARS1), Met (MARS1) the bifunctional ligase for Glu and Pro (EPRS1) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18 (PubMed:19131329, PubMed:19289464, PubMed:26472928, Ref.26). Forms a linear complex that contains MARS1, EEF1E1, EPRS1 and AIMP2 that is at the core of the multisubunit complex (PubMed:26472928)

Research Field

Signal Transduction

Synonyms

MRS; ILLD; CMT2U; ILFS2; METRS; MTRNS; SPG70

Documentation

User Guide for Antibodies (1077 KB)