EGFR Antibody (YA6410)
(Synonyms: EGFR; Avian erythroblastic leukemia viral(v erb b) oncogene homolog; Avian erythroblastic leukemia viral(verbb) oncogene homolog; Cell growth inhibiting protein 40; Cell proliferation inducing protein 61; EGF R; EGFR; Epidermal growth factor receptor(avian erythroblastic leukemia viral(v erb b) oncogene homolog); Epidermal growth factor receptor(erythroblastic leukemia viral(v erb b) oncogene homolog avian); Epidermal growth factor receptor; erbb 1; Erbb; Erbb1; HER1; mENA; Oncogene ERBB; PIG61; Receptor tyrosine protein kinase ErbB 1; Receptor tyrosine protein kinase ErbB1; Urogastrone; wa2; Wa5; EGFR_HUMAN.)EGFR Antibody (YA6410) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to EGFR.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, IHC-P, IHC-F, IF-Tissue
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Reactivity :
Human
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Formulation:
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:100-500 | 1:100-500 | 1:100-500 |
Product Details
EGFR Antibody (YA6410) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to EGFR.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman Predicted Reactivity: Dog,Cow,Sheep
Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
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Calculated Molecular Weight Predicted band size: 130 kDa
KLH conjugated synthetic peptide derived from human EGFR
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
The EGFR family of receptor tyrosine kinases (RTK) comprises four distinct receptors: the EGFR (also known as ErbB-1/HER1), ErbB-2 (neu, HER2), ErbB-3 (HER3) and ErbB-4 (HER4) . All EGFR family members are characterized by a modular structure consisting of an extracellular ligand-binding domain, a single hydrophobic transmembrane region, and the intracellular part harbouring the highly conserved tyrosine kinase domain. The ErbB family of receptor tyrosine kinases (RTKs) couples binding of extracellular growth factor ligands to intracellular signaling pathways regulating diverse biologic responses, including proliferation, differentiation, cell motility, and survival. Ten growth factors and their ErbB specificities are: EGF, amphiregulin (AR), and TGF bind ErbB-1; betacellulin, and epiregulin bind both ErbB-1 and ErbB-4; the neuregulins (also called heregulins and Neu differentiation factors) NRG-1 and NRG-2 bind ErbB-3 and ErbB-4; and NRG-3 and NRG-4 bind ErbB-4. No known ligand binds ErbB-2. The three best characterized signaling pathways induced through ErbBs are Ras-mitogen-activated protein kinase (Ras-MAPK), phosphatidylinositol 3 kinase-protein kinase B (PI3K-PKB/Akt), and phospholipase C-protein kinase C (PLC-PKC) pathways.
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Subcellular Localization
Cell membrane,Endoplasmic reticulum membrane,Golgi apparatus membrane,Nucleus membrane,Endosome,Endosome membrane,Nucleus,Secreted
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Expression
Tissue_Specificity: Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers -
Isoforms & Post-Translational Modification
P00533 has four isomers: P00533-1: 134277 Da (predicted); P00533-2: 44664 Da (predicted); P00533-3: 77312 Da (predicted); P00533-4: 69228 Da (predicted).
Phosphorylated on Tyr residues in response to EGF (PubMed:20462955, PubMed:27153536)丨Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting (PubMed:27153536)丨Palmitoylated on Cys residues by ZDHHC20丨Methylated -
Subunit
Binding of the ligand triggers homo- and/or heterodimerization of the receptor triggering its autophosphorylation
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SwissProt ID
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Synonyms
EGFR; Avian erythroblastic leukemia viral(v erb b) oncogene homolog; Avian erythroblastic leukemia viral(verbb) oncogene homolog; Cell growth inhibiting protein 40; Cell proliferation inducing protein 61; EGF R; EGFR; Epidermal growth factor receptor(avian erythroblastic leukemia viral(v erb b) oncogene homolog); Epidermal growth factor receptor(erythroblastic leukemia viral(v erb b) oncogene homolog avian); Epidermal growth factor receptor; erbb 1; Erbb; Erbb1; HER1; mENA; Oncogene ERBB; PIG61; Receptor tyrosine protein kinase ErbB 1; Receptor tyrosine protein kinase ErbB1; Urogastrone; wa2; Wa5; EGFR_HUMAN.
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