EGFR Antibody (YA6410)

Cat. No.: HY-P86718: Data Sheet User Guide for Antibodies
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EGFR Antibody (YA6410) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to EGFR.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

EGFR Antibody (YA6410) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to EGFR.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 130 kDa

Species Reactivity

Human Predicted Reactivity: Dog,Cow,Sheep

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

P00533

Gene ID

1956 [NCBI]

Immunogen

KLH conjugated synthetic peptide derived from human EGFR

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:10805725, PubMed:27153536, PubMed:2790960, PubMed:35538033). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed:12297049, PubMed:15611079, PubMed:17909029, PubMed:20837704, PubMed:27153536, PubMed:2790960, PubMed:7679104, PubMed:8144591, PubMed:9419975). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed:27153536). May also activate the NF-kappa-B signaling cascade (PubMed:11116146).

Subcellular Localization：Cell membrane,Endoplasmic reticulum membrane,Golgi apparatus membrane,Nucleus membrane,Endosome,Endosome membrane,Nucleus,Secreted

Expression：
Tissue_Specificity: Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers

Isoforms & Post-Translational Modification：P00533 has four isomers: P00533-1: 134277 Da (predicted); P00533-2: 44664 Da (predicted); P00533-3: 77312 Da (predicted); P00533-4: 69228 Da (predicted).
Phosphorylated on Tyr residues in response to EGF (PubMed:20462955, PubMed:27153536)丨Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting (PubMed:27153536)丨Palmitoylated on Cys residues by ZDHHC20丨Methylated

Subunit：Binding of the ligand triggers homo- and/or heterodimerization of the receptor triggering its autophosphorylation

Synonyms

EGFR; Avian erythroblastic leukemia viral(v erb b) oncogene homolog; Avian erythroblastic leukemia viral(verbb) oncogene homolog; Cell growth inhibiting protein 40; Cell proliferation inducing protein 61; EGF R; EGFR; Epidermal growth factor receptor(avian erythroblastic leukemia viral(v erb b) oncogene homolog); Epidermal growth factor receptor(erythroblastic leukemia viral(v erb b) oncogene homolog avian); Epidermal growth factor receptor; erbb 1; Erbb; Erbb1; HER1; mENA; Oncogene ERBB; PIG61; Receptor tyrosine protein kinase ErbB 1; Receptor tyrosine protein kinase ErbB1; Urogastrone; wa2; Wa5; EGFR_HUMAN.

Documentation

User Guide for Antibodies (1077 KB)