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PLEKHA1 Antibody (YA2145)

(Synonyms: PH domain-containing family A member 1)
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Based on 1 Customer Validation

PLEKHA1 Antibody (YA2145) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PLEKHA1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PLEKHA1 Antibody (YA2145)
  • PLEKHA1 Antibody (YA2145) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

PLEKHA1 Antibody (YA2145) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PLEKHA1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 46 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 46 kDa
Species Reactivity Database

Entrez Gene: 59338 Human

SwissProt: Q9HB21 Human

Immunogen

A synthetic peptide of human TAPP-1

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104509

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PLEKHA1 Antibody (YA2145)
    Western blot analysis of extracts from Jurkat (lane 2, 20ug), Hela (lane 3, 20ug), K562 (lane 4, 20ug) and SiHa (lane 5, 20ug) using PLEKHA1 Antibody (HY-P82400). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    PLEKHA1 binds specifically to phosphatidylinositol 3,4-diphosphate (PtdIns3,4P2), but not to other phosphoinositides. May recruit other proteins to the plasma membrane

  • Subcellular Localization

    Cytoplasm; Cell membrane; Peripheral membrane protein; Nucleus

  • Expression


    Tissue_specificity:Highly expressed in skeletal muscle, thymus, pancreas, placenta and lung. Detected at low levels in brain, heart, peripheral blood leukocytes, testis, ovary, spinal cord, thyroid, kidney, liver, small intestine and colon

  • Isoforms & Post-Translational Modification

    Q9HB21 has 2 isomers: Q9HB21-1: 45553 Da (predicted); Q9HB21-2: 38113 Da (predicted).

  • Subunit

    Interacts with MPDZ and PTPN13

  • SwissProt ID

    Q9HB21

  • Gene ID
    59338 [NCBI]
  • Synonyms

    PH domain-containing family A member 1

  • Research Field

    Signal Transduction

PLEKHA1 Antibody (YA2145) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2, 20ug), Hela (lane 3, 20ug), K562 (lane 4, 20ug) and SiHa (lane 5, 20ug) using PLEKHA1 Antibody (HY-P82400). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • PLEKHA1 Antibody (YA2145) Product Image
    1/16
  • Experimental Validation Results for PLEKHA1 Antibody (YA2145)
  • PLEKHA1 Antibody (YA2145) Product Image
  • Experimental Validation Results for PLEKHA1 Antibody (YA2145)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2, 20ug), Hela (lane 3, 20ug), K562 (lane 4, 20ug) and SiHa (lane 5, 20ug) using PLEKHA1 Antibody (HY-P82400). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PLEKHA1 Antibody (YA2145)
  • PLEKHA1 Antibody (YA2145) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat (lane 2, 20ug), Hela (lane 3, 20ug), K562 (lane 4, 20ug) and SiHa (lane 5, 20ug) using PLEKHA1 Antibody (HY-P82400). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • PLEKHA1 Antibody (YA2145) Product Image WB