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Thrombopoietin Receptor Antibody (YA2241)

(Synonyms: MPL; TPOR; Thrombopoietin receptor; TPO-R; Myeloproliferative leukemia protein; Proto-oncogene c-Mpl; CD110)
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Based on 1 Customer Validation

Thrombopoietin Receptor Antibody (YA2241) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Thrombopoietin Receptor.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Thrombopoietin Receptor Antibody (YA2241)
  • Thrombopoietin Receptor Antibody (YA2241) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:1000-1:5000

Product Details

Description

Thrombopoietin Receptor Antibody (YA2241) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Thrombopoietin Receptor.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 85 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 71 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human TPOR

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Thrombopoietin Receptor Antibody (YA2241)
    Western blot analysis of extracts from NIH/3T3 (lane2, 20μg), Rat spleen tissue (lane3, 40μg) and Mouse spleen tissue (lane4, 40μg) using Thrombopoietin Receptor Antibody (HY-P82496). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Thrombopoietin (TPO) is the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.

    TPO binds to the thrombopoietin receptor (TPOr, also termed c-mpl) on platelets, megakaryocytes, and pluripotent stem cells leading to inhibition of apoptosis of stem cells and megakaryocytes; increased megakaryocyte number, size, and ploidy; increased rate of megakaryocyte maturation and platelet count; and decreased platelet threshold for activation by ADP and collagen.

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Golgi apparatus; Cell surface

  • Expression


    Tissue_specificity:It is expressed at low levels in a large number of hematopoietic cells. Isomer 1 and isomer 2 are always co-expressed.

  • Isoforms & Post-Translational Modification

    P40238 has 2 isomers: P40238-1: 71245 Da (predicted); P40238-2: 65521 Da (predicted).
    Phosphorylated at Tyr-591 in response to THPO stimulation;Ubiquitination at Lys-553 and Lys-573 targets MPL for degradation by both the lysosomal and proteasomal pathways. The E3 ubiquitin-protein ligase CBL significantly contributes to this ubiquitination

  • Subunit

    Homodimer (PubMed:32029621, PubMed:25538044). Interacts with ATXN2L (PubMed:11784712). Interacts with JAK2 and TYK2; these interactions increase MPL localization to the cell membrane (PubMed:15899890). Interacts with THPO (PubMed:37633268). Interacts with SHIP/INPP5D (PubMed:24607955). Interacts with BTK (PubMed:24607955). Interacts with SYK; this interaction negatively regulates THPO-mediated ERK1/2 signaling (PubMed:24607955)

  • SwissProt ID

    P40238

  • Gene ID
    4352 [NCBI]
  • Synonyms

    MPL; TPOR; Thrombopoietin receptor; TPO-R; Myeloproliferative leukemia protein; Proto-oncogene c-Mpl; CD110

  • Research Field

    Immunology

Thrombopoietin Receptor Antibody (YA2241) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from NIH/3T3 (lane2, 20μg), Rat spleen tissue (lane3, 40μg) and Mouse spleen tissue (lane4, 40μg) using Thrombopoietin Receptor Antibody (HY-P82496). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Thrombopoietin Receptor Antibody (YA2241) Product Image
    1/16
  • Experimental Validation Results for Thrombopoietin Receptor Antibody (YA2241)
  • Thrombopoietin Receptor Antibody (YA2241) Product Image
  • Experimental Validation Results for Thrombopoietin Receptor Antibody (YA2241)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from NIH/3T3 (lane2, 20μg), Rat spleen tissue (lane3, 40μg) and Mouse spleen tissue (lane4, 40μg) using Thrombopoietin Receptor Antibody (HY-P82496). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Thrombopoietin Receptor Antibody (YA2241)
  • Thrombopoietin Receptor Antibody (YA2241) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from NIH/3T3 (lane2, 20μg), Rat spleen tissue (lane3, 40μg) and Mouse spleen tissue (lane4, 40μg) using Thrombopoietin Receptor Antibody (HY-P82496). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Thrombopoietin Receptor Antibody (YA2241) Product Image WB