Copy Link Print Information

SNX1 Antibody (YA2044)

(Synonyms: SNX1; Sorting nexin-1)
Customer Review

Based on 1 Customer Validation

SNX1 Antibody (YA2044) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SNX1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC FC
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • SNX1 Antibody (YA2044) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50-1:100

Product Details

Description

SNX1 Antibody (YA2044) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SNX1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 74 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 59 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human SNX1 aa50-100/522.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104410

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SNX1 Antibody (YA2044)
    Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), HepG2 (lane 2, 20 μg), 293T (lane 3, 20 μg), Jurkat (lane 4, 20 μg), and U2OS (lane 5, 20 μg) using SNX1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for SNX1 Antibody (YA2044)
    Immunohistochemical analysis of paraffin-embedded human Glioma‌ tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SNX1 Antibody (YA2044)
    Immunohistochemical analysis of paraffin-embedded human Liver cyst tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SNX1 Antibody (YA2044)
    Immunohistochemical analysis of paraffin-embedded human pancreatic serous cystadenoma‌ tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SNX1 Antibody (YA2044)
    Immunohistochemical analysis of paraffin-embedded human Hepatocellular Carcinoma tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SNX1 Antibody (YA2044)
    Immunohistochemical analysis of paraffin-embedded human Kimura's disease tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SNX1 Antibody (YA2044)
    Immunohistochemical analysis of paraffin-embedded human adenolymphoma tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SNX1 Antibody (YA2044)
    Immunohistochemical analysis of paraffin-embedded human Mucoepidermoid carcinoma tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SNX1 Antibody (YA2044)
    Immunohistochemical analysis of paraffin-embedded human Follicular Lymphoma tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SNX1 Antibody (YA2044)
    Immunohistochemical analysis of paraffin-embedded human solitary fibrous tumor tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SNX1 Antibody (YA2044)
    Immunohistochemical analysis of paraffin-embedded human cervical squamous cell carcinoma tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SNX1 Antibody (YA2044)
    Immunocytochemistry analysis of HepG2 cells labeling SNX1 with SNX1 Antibody (HY-P82299) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SNX1 Antibody (HY-P82299) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SNX1 Antibody (YA2044)
    Immunocytochemistry analysis of NIH-3T3 cells labeling SNX1 with SNX1 Antibody (HY-P82299) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SNX1 Antibody (HY-P82299) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SNX1 Antibody (YA2044)
    Flow cytometric analysis of 1X106 HeLa cells labeling SNX1 Antibody (HY-P82299, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    SNX1 is involved in several stages of intracellular trafficking. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier (ETC) (Probable). Can sense membrane curvature and has in vitro vesicle-to-membrane remodeling activity. Involved in retrograde endosome-to-TGN transport of lysosomal enzyme receptors (IGF2R, M6PR and SORT1) and Shiginella dysenteria toxin stxB. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Involvement in retromer-independent endocytic trafficking of P2RY1 and lysosomal degradation of protease-activated receptor-1/F2R. Promotes KALRN- and RHOG-dependent but retromer-independent membrane remodeling such as lamellipodium formation; the function is dependent on GEF activity of KALRN. Required for endocytosis of DRD5 upon agonist stimulation but not for basal receptor trafficking[1][2][3][4][5][6][7][8][9][10][11][12].

  • Subcellular Localization

    Endosome membrane; Peripheral membrane protein; Cytoplasmic side; Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein; Cytoplasmic side; Early endosome membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection, lamellipodium

  • Isoforms & Post-Translational Modification

    Q13596 has 3 isomers: Q13596-1: 59070 Da (predicted); Q13596-2: 51813 Da (predicted); Q13596-3: 63082 Da (predicted).

  • Subunit

    Predominantly forms heterodimers with BAR domain-containing sorting nexins SNX5, SNX6 and SNX32; can self-associate to form homodimers (PubMed:23085988). The heterodimers are proposed to self-assemble into helical arrays on the membrane to stabilize and expand local membrane curvature underlying endosomal tubule formation. Thought to be a component of the originally described retromer complex (also called SNX-BAR retromer) which is a pentamer containing the heterotrimeric retromer cargo-selective complex (CSC), also described as vacuolar protein sorting subcomplex (VPS) and a heterodimeric membrane-deforming subcomplex formed between SNX1 or SNX2 and SNX5 or SNX6 (also called SNX-BAR subcomplex); the respective CSC and SNX-BAR subcomplexes associate with low affinity (Probable). Interacts with SNX5, SNX6, SNX32, VPS26A, VPS29, VPS35, DRD5, DENND5A, KALRN, RHOG (GDP-bound form) (PubMed:11102511, PubMed:19619496, PubMed:19935774, PubMed:20604901, PubMed:23085988, PubMed:23152498, PubMed:9819414). The interaction with SNX2 is reported controversially (PubMed:11997453, PubMed:19619496, PubMed:23085988, PubMed:9819414). Interacts with DNAJC13; prevented by presence of HGS (PubMed:19874558). Interacts with HGS (By similarity)

  • SwissProt ID

    Q13596

  • Gene ID
    6642 [NCBI]
  • Synonyms

    SNX1; Sorting nexin-1

  • Research Field

    Signal Transduction

SNX1 Antibody (YA2044) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), HepG2 (lane 2, 20 μg), 293T (lane 3, 20 μg), Jurkat (lane 4, 20 μg), and U2OS (lane 5, 20 μg) using SNX1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Glioma‌ tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Liver cyst tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreatic serous cystadenoma‌ tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Hepatocellular Carcinoma tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Kimura's disease tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human adenolymphoma tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Mucoepidermoid carcinoma tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Follicular Lymphoma tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human solitary fibrous tumor tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervical squamous cell carcinoma tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling SNX1 with SNX1 Antibody (HY-P82299) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SNX1 Antibody (HY-P82299) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of NIH-3T3 cells labeling SNX1 with SNX1 Antibody (HY-P82299) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SNX1 Antibody (HY-P82299) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling SNX1 Antibody (HY-P82299, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • SNX1 Antibody (YA2044) Product Image
    1/16
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • SNX1 Antibody (YA2044) Product Image
  • Experimental Validation Results for SNX1 Antibody (YA2044)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), HepG2 (lane 2, 20 μg), 293T (lane 3, 20 μg), Jurkat (lane 4, 20 μg), and U2OS (lane 5, 20 μg) using SNX1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for SNX1 Antibody (YA2044)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Glioma‌ tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SNX1 Antibody (YA2044)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Liver cyst tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SNX1 Antibody (YA2044)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreatic serous cystadenoma‌ tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SNX1 Antibody (YA2044)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Hepatocellular Carcinoma tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SNX1 Antibody (YA2044)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Kimura's disease tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SNX1 Antibody (YA2044)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human adenolymphoma tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SNX1 Antibody (YA2044)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Mucoepidermoid carcinoma tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SNX1 Antibody (YA2044)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Follicular Lymphoma tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SNX1 Antibody (YA2044)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human solitary fibrous tumor tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SNX1 Antibody (YA2044)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervical squamous cell carcinoma tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for SNX1 Antibody (YA2044)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling SNX1 with SNX1 Antibody (HY-P82299) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SNX1 Antibody (HY-P82299) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SNX1 Antibody (YA2044)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of NIH-3T3 cells labeling SNX1 with SNX1 Antibody (HY-P82299) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SNX1 Antibody (HY-P82299) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SNX1 Antibody (YA2044)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling SNX1 Antibody (HY-P82299, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • Experimental Validation Results for SNX1 Antibody (YA2044)
  • SNX1 Antibody (YA2044) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), HepG2 (lane 2, 20 μg), 293T (lane 3, 20 μg), Jurkat (lane 4, 20 μg), and U2OS (lane 5, 20 μg) using SNX1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • SNX1 Antibody (YA2044) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Glioma‌ tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SNX1 Antibody (YA2044) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Liver cyst tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SNX1 Antibody (YA2044) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human pancreatic serous cystadenoma‌ tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SNX1 Antibody (YA2044) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Hepatocellular Carcinoma tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SNX1 Antibody (YA2044) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Kimura's disease tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SNX1 Antibody (YA2044) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human adenolymphoma tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SNX1 Antibody (YA2044) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Mucoepidermoid carcinoma tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SNX1 Antibody (YA2044) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Follicular Lymphoma tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SNX1 Antibody (YA2044) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human solitary fibrous tumor tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SNX1 Antibody (YA2044) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cervical squamous cell carcinoma tissue using SNX1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82299, 1:200 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • SNX1 Antibody (YA2044) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling SNX1 with SNX1 Antibody (HY-P82299) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SNX1 Antibody (HY-P82299) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • SNX1 Antibody (YA2044) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of NIH-3T3 cells labeling SNX1 with SNX1 Antibody (HY-P82299) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SNX1 Antibody (HY-P82299) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • SNX1 Antibody (YA2044) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 HeLa cells labeling SNX1 Antibody (HY-P82299, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • SNX1 Antibody (YA2044) Product Image WB
  • SNX1 Antibody (YA2044) Product Image IHC-P
  • SNX1 Antibody (YA2044) Product Image IHC-P
  • SNX1 Antibody (YA2044) Product Image IHC-P
  • SNX1 Antibody (YA2044) Product Image IHC-P
  • SNX1 Antibody (YA2044) Product Image IHC-P
  • SNX1 Antibody (YA2044) Product Image IHC-P
  • SNX1 Antibody (YA2044) Product Image IHC-P
  • SNX1 Antibody (YA2044) Product Image IHC-P
  • SNX1 Antibody (YA2044) Product Image IHC-P
  • SNX1 Antibody (YA2044) Product Image IHC-P
  • SNX1 Antibody (YA2044) Product Image ICC
  • SNX1 Antibody (YA2044) Product Image ICC
  • SNX1 Antibody (YA2044) Product Image FC