ETS1 Antibody (YA2494)(PBS only)

Cat. No.: HY-P82749A: Data Sheet User Guide for Antibodies
FAQs
Technical Support

ETS1 Antibody (YA2494) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ETS1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

ETS1 Antibody (YA2494) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ETS1.

Host

Rabbit

Molecular Weight

Predicted band size: 50 kDa;

Observed band size: 50 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P14921

Gene ID

2113 [NCBI]

Immunogen

A synthetic peptide of human ETS1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Transcription factor (PubMed:10698492, PubMed:11909962). Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts (PubMed:20378371). May control the differentiation, survival and proliferation of lymphoid cells (PubMed:20378371). May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion (PubMed:15247905, PubMed:15592518); Acts as a dominant-negative for isoform c-ETS-1A

Subcellular Localization：Nucleus; Cytoplasm

Expression：
Tissue_specificity:It is highly expressed in lymphocytes. Both c-ETS-1A and Ets-1 p27 subtypes were detected in all fetal tissues tested, but in human tissues, the variation varied depending on the tissue type. It was not detected in brain tissue or kidneys.

Induction:Up-regulated by retinoic acid, VEGF, TNF-alpha/TNFA, lipopolysaccharide and in response to hypoxia (at protein level)

Isoforms & Post-Translational Modification：P14921 has 5 isomers: P14921-1: 50408 Da (predicted); P14921-2: 40786 Da (predicted); P14921-3: 55412 Da (predicted); P14921-4: 25697 Da (predicted); P14921-5: 30860 Da (predicted).
Sumoylated on Lys-15 and Lys-227, preferentially with SUMO2; which inhibits transcriptional activity;Ubiquitinated; which induces proteasomal degradation;Phosphorylation at Ser-251, Ser-282 and Ser-285 by CaMK2/CaMKII in response to calcium signaling decreases affinity for DNA: an increasing number of phosphoserines causes DNA-binding to become progressively weaker

Subunit：Binds DNA as a homodimer; homodimerization is required for transcription activation (PubMed:18566588). Interacts with MAF and MAFB (By similarity). Interacts with PAX5; the interaction alters DNA-binding properties (By similarity). Interacts with DAXX (PubMed:10698492). Interacts with UBE2I (PubMed:9333025). Interacts with SP100; the interaction is direct and modulates ETS1 transcriptional activity (PubMed:11909962, PubMed:15247905)

Research Field

Epigenetics and Nuclear Signaling

Synonyms

ETS1; EWSR2; Protein C-ets-1; p54

Documentation

User Guide for Antibodies (1077 KB)