SARA Antibody (YA3226)(PBS only)

Cat. No.: HY-P83481A: Data Sheet User Guide for Antibodies
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SARA Antibody (YA3226) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SARA.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

SARA Antibody (YA3226) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SARA.

Host

Rabbit

Molecular Weight

Predicted band size: 156 kDa;

Observed band size: 156 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

O95405

Gene ID

9372 [NCBI]

Immunogen

A synthetic peptide of human SARA

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000-1:5000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Early endosomal protein that functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. Plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. Possibly associated with TGF-beta receptor internalization

Subcellular Localization：Cytoplasm; Early endosome membrane

Expression：
Tissue_specificity:Ubiquitous. In the brain found primarily in the cerebrovascular smooth muscle cells and reactive astrocytes

Isoforms & Post-Translational Modification：O95405 has 3 isomers: O95405-1: 156403 Da (predicted); O95405-2: 150348 Da (predicted); O95405-3: 83721 Da (predicted).

Subunit：Interacts (via the SBD region) with SMAD2; the interaction recruits SMAD2 to the TGF-beta receptor and is disrupted by phosphorylation of SMAD2 upon TGF-beta receptor activation. Interacts with SMAD3. Interacts with TGFBR1 and TGFBR2; the interaction recruits SMAD2 to the TGF-beta receptor. Interacts with PML

Research Field

Signal Transduction

Synonyms

NSP; SARA; MADHIP; SMADIP; PPP1R173

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/cho-cell.html [Content Brief]