Phospho-PAK4/5/6 (Ser474/Ser560/Ser602) Antibody (YA3333)(PBS only)

Cat. No.: HY-P83588A: Data Sheet User Guide for Antibodies
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Phospho-PAK4/5/6 (Ser474/Ser560/Ser602) Antibody (YA3333) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-PAK4/5/6 (Ser474/Ser560/Ser602).

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

Phospho-PAK4/5/6 (Ser474/Ser560/Ser602) Antibody (YA3333) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-PAK4/5/6 (Ser474/Ser560/Ser602).

Host

Rabbit

Molecular Weight

Predicted band size: 64,80,75 kDa;

Observed band size: 64,80,75 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O96013 / Q9P286 / Q9NQU5

Gene ID

10298 [NCBI] / 57144 [NCBI] / 56924 [NCBI]

Immunogen

A synthesized peptide derived from human Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602)

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：O96013: Serine/threonine-protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell adhesion turnover, cell migration, growth, proliferation or cell survival (PubMed:26598620). Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin.
Q9P286: Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity. Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Phosphorylates CTNND1, probably to regulate cytoskeletal organization and cell morphology. Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions
Q9NQU5: Serine/threonine protein kinase that plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including AR or MAP2K6/MAPKK6. Phosphorylates the DNA-binding domain of androgen receptor/AR and thereby inhibits AR-mediated transcription. Also inhibits ESR1-mediated transcription. May play a role in cytoskeleton regulation by interacting with IQGAP1. May protect cells from apoptosis through phosphorylation of BAD

Subcellular Localization：O96013: Cytoplasm
Q9P286: Mitochondrion; Cytoplasm; Nucleus
Q9NQU5: Cytoplasm; Nucleus

Expression：
Tissue_specificity: O96013: Highest expression in prostate, testis and colon
Q9P286: Predominantly expressed in brain
Q9NQU5: Selectively expressed in brain and testis, with lower levels in multiple tissues including prostate and breast

Subunit：O96013: Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and weakly with RAC1 (PubMed:15827085, PubMed:26598620). Interacts with INKA1 (PubMed:26607847)
Q9P286: Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. Interacts with MARK2, leading to inhibit MARK2 independently of kinase activity. Interacts with RHOD and RHOH
Q9NQU5: Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1 (By similarity). Interacts with the androgen receptor AR and the estrogen receptor ESR1. Interacts with IQGAP1 and PPM1B

Research Field

Signal Transduction

Synonyms

PAK4; PAK5; PAK6

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/mt4-cell.html [Content Brief]