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Alpha-ENaC Antibody

(Synonyms: Alpha ENaC 2; Alpha ENaC; Alpha NaCH; Alpha-NaCH; Amiloride sensitive epithelial sodium channel alpha subunit; Amiloride sensitive sodium channel subunit alpha; Amiloride-sensitive sodium channel subunit alpha; ENaCa; ENaCalpha; Epithelial Na(+) channel subunit alpha; Epithelial Na+ channel subunit alpha; FLJ21883; Nonvoltage gated sodium channel 1 subunit alpha; Nonvoltage-gated sodium channel 1 subunit alpha; SCNEA; SCNN 1; SCNN1; SCNN1A; SCNNA_HUMAN; Sodium channel nonvoltage gated 1 alpha.)
Customer Review

Based on 1 Customer Validation

Alpha-ENaC Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Alpha-ENaC.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ELISA, IHC-P, IHC-F, FC, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Alpha-ENaC Antibody
  • Experimental Validation Results for Alpha-ENaC Antibody
  • Alpha-ENaC Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
FC Info
FC: Flow Cytometry
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-2000 1:5000-10000 1:100-500 1:100-500 5ug:Test 1:100-500

Product Details

Description

Alpha-ENaC Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Alpha-ENaC.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat Predicted Reactivity: Dog,Pig,Cow,Horse,Sheep
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 80 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 76 kDa
Species Reactivity Database
Immunogen

KLH conjugated synthetic peptide derived from human Alpha-ENaC: 201-300/669

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103273

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Alpha-ENaC Antibody
    Immunocytochemistry analysis of Hela cells labeling Alpha-ENaC with Alpha-ENaC Antibody (HY-P81204) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Alpha-ENaC Antibody (HY-P81204) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Alpha-ENaC Antibody
    Immunocytochemistry analysis of Hela cells labeling Alpha-ENaC with Alpha-ENaC Antibody (HY-P81204) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Alpha-ENaC Antibody (HY-P81204) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Alpha-ENaC is a This is one of the three pore-forming subunits of the heterotrimeric epithelial sodium channel (ENaC), a critical regulator of sodium balance and fluid homeostasis. ENaC operates in epithelial tissues, where it mediates the electrodiffusion of sodium ions from extracellular fluid through the apical membrane of cells, with water following osmotically. It plays a key role in maintaining sodium homeostasis through electrogenic sodium reabsorption in the kidneys. Additionally, ENaC is essential for airway surface liquid homeostasis, which is crucial for proper mucus clearance; Not functional[1][2][3][4][5][6][7][8].

  • Subcellular Localization

    Apical cell membrane; Multi-pass membrane protein; Cell projection, cilium; Cytoplasmic granule; Cytoplasm; Cytoplasmic vesicle, secretory vesicle, acrosome; Cell projection, cilium, flagellum

  • Expression


    Tissue_specificity:Expressed in the female reproductive tract, from the fimbrial end of the fallopian tube to the endometrium (at protein level) (PubMed:22207244) . Expressed in kidney (at protein level) . In the respiratory tract, expressed in the bronchial epithelium (at protein level) . Highly expressed in lung. Detected at intermediate levels in pancreas and liver, and at low levels in heart and placenta (PubMed:22207244) . in skin, expressed in keratinocytes, melanocytes and Merkel cells of the epidermal sub-layers, stratum basale, stratum spinosum and stratum granulosum (at protein level) (PubMed:28130590) . Expressed in the outer root sheath of the hair follicles (at protein level) (PubMed:28130590) . Detected in both peripheral and central cells of the sebaceous gland (at protein level) (PubMed:28130590) . Expressed by eccrine sweat glands (at protein level) (PubMed:28130590) . In skin, also expressed by arrector pili muscle cells and intradermal adipocytes (PubMed:28130590) . Isoform 1 and isoform 2 predominate in all tissues; Detected in lung and heart

    Induction:By aldosterone

  • Isoforms & Post-Translational Modification

    P37088 has 6 isomers: P37088-1: 75704 Da (predicted); P37088-2: 81856 Da (predicted); P37088-3: 28328 Da (predicted); P37088-4: 73603 Da (predicted); P37088-5: 77980 Da (predicted); P37088-6: 78234 Da (predicted).
    Ubiquitinated. Can be ubiquitinated at multiple sites and undergo monoubiquitination and polyubiquitination. Ubiquitination by NEDD4 or NEDD4L inhibits the ENaC channel through endocytosis, intracellular retention and degradation of its individual subunits;ENaC is activated through the proteolytic maturation of its subunits. Furin cleaves the SCNN1A subunit, which results in a stepwise increase in the open probability of the channel due to the release of an inhibitory tract (By similarity). BPIFA1, which is recruited by the SCNN1B subunit, prevents the proteolytic activation of ENaC (PubMed:24124190);N-glycosylated

  • Subunit

    Heterotrimer; containing an alpha/SCNN1A, a beta/SCNN1B and a gamma/SCNN1G subunit (PubMed:30251954, PubMed:32729833). Interacts with WWP1 (via WW domains) (PubMed:9169421). Interacts with WWP2 (via WW domains); inhibits the channel (PubMed:12167593, PubMed:9169421). Interacts with BPIFA1; the interaction is indirect via SCNN1B and inhibits the proteolytic processing of SCNN1A and SCNN1G and the activation of ENaC (PubMed:24124190). Interacts with the full-length immature form of PCSK9 (pro-PCSK9); inhibits ENaC by promoting its proteasomal degradation (PubMed:22493497)

  • SwissProt ID

    P37088

  • Gene ID
    6337 [NCBI]
  • Synonyms

    Alpha ENaC 2; Alpha ENaC; Alpha NaCH; Alpha-NaCH; Amiloride sensitive epithelial sodium channel alpha subunit; Amiloride sensitive sodium channel subunit alpha; Amiloride-sensitive sodium channel subunit alpha; ENaCa; ENaCalpha; Epithelial Na(+) channel subunit alpha; Epithelial Na+ channel subunit alpha; FLJ21883; Nonvoltage gated sodium channel 1 subunit alpha; Nonvoltage-gated sodium channel 1 subunit alpha; SCNEA; SCNN 1; SCNN1; SCNN1A; SCNNA_HUMAN; Sodium channel nonvoltage gated 1 alpha.

Alpha-ENaC Antibody Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Alpha-ENaC with Alpha-ENaC Antibody (HY-P81204) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Alpha-ENaC Antibody (HY-P81204) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Alpha-ENaC with Alpha-ENaC Antibody (HY-P81204) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Alpha-ENaC Antibody (HY-P81204) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Alpha-ENaC Antibody Product Image
    1/16
  • Experimental Validation Results for Alpha-ENaC Antibody
  • Experimental Validation Results for Alpha-ENaC Antibody
  • Alpha-ENaC Antibody Product Image
  • Experimental Validation Results for Alpha-ENaC Antibody
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Alpha-ENaC with Alpha-ENaC Antibody (HY-P81204) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Alpha-ENaC Antibody (HY-P81204) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Alpha-ENaC Antibody
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Alpha-ENaC with Alpha-ENaC Antibody (HY-P81204) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Alpha-ENaC Antibody (HY-P81204) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Alpha-ENaC Antibody
  • Experimental Validation Results for Alpha-ENaC Antibody
  • Alpha-ENaC Antibody Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Alpha-ENaC with Alpha-ENaC Antibody (HY-P81204) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Alpha-ENaC Antibody (HY-P81204) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Alpha-ENaC Antibody Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Alpha-ENaC with Alpha-ENaC Antibody (HY-P81204) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Alpha-ENaC Antibody (HY-P81204) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Alpha-ENaC Antibody Product Image ICC
  • Alpha-ENaC Antibody Product Image ICC