Alpha-ENaC Antibody

Cat. No.: HY-P81204: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Alpha-ENaC Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Alpha-ENaC.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

Alpha-ENaC Antibody Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Alpha-ENaC Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Alpha-ENaC.

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 76 kDa;

Observed band size: 80 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat Predicted Reactivity: Dog, Pig, Cow, Horse, Sheep

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

P37088

Gene ID

6337 [NCBI]

Immunogen

KLH conjugated synthetic peptide derived from human Alpha-ENaC: 201-300/669

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-10000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
FC FC: Flow Cytometry	5ug:Test
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：This is one of the three pore-forming subunits of the heterotrimeric epithelial sodium channel (ENaC), a critical regulator of sodium balance and fluid homeostasis (PubMed:30251954, PubMed:32729833, PubMed:8023962, PubMed:8278374, PubMed:9792722). ENaC operates in epithelial tissues, where it mediates the electrodiffusion of sodium ions from extracellular fluid through the apical membrane of cells, with water following osmotically (PubMed:24124190, PubMed:28710092, PubMed:8278374). It plays a key role in maintaining sodium homeostasis through electrogenic sodium reabsorption in the kidneys (PubMed:12107247). Additionally, ENaC is essential for airway surface liquid homeostasis, which is crucial for proper mucus clearance (PubMed:24124190, PubMed:28710092); Not functional

Subcellular Localization：Apical cell membrane; Multi-pass membrane protein; Cell projection, cilium; Cytoplasmic granule; Cytoplasm; Cytoplasmic vesicle, secretory vesicle, acrosome; Cell projection, cilium, flagellum

Expression：
Tissue_specificity:Expressed in the female reproductive tract, from the fimbrial end of the fallopian tube to the endometrium (at protein level) (PubMed:22207244) . Expressed in kidney (at protein level) . In the respiratory tract, expressed in the bronchial epithelium (at protein level) . Highly expressed in lung. Detected at intermediate levels in pancreas and liver, and at low levels in heart and placenta (PubMed:22207244) . in skin, expressed in keratinocytes, melanocytes and Merkel cells of the epidermal sub-layers, stratum basale, stratum spinosum and stratum granulosum (at protein level) (PubMed:28130590) . Expressed in the outer root sheath of the hair follicles (at protein level) (PubMed:28130590) . Detected in both peripheral and central cells of the sebaceous gland (at protein level) (PubMed:28130590) . Expressed by eccrine sweat glands (at protein level) (PubMed:28130590) . In skin, also expressed by arrector pili muscle cells and intradermal adipocytes (PubMed:28130590) . Isoform 1 and isoform 2 predominate in all tissues; Detected in lung and heart

Induction:By aldosterone

Isoforms & Post-Translational Modification：P37088 has 6 isomers: P37088-1: 75704 Da (predicted); P37088-2: 81856 Da (predicted); P37088-3: 28328 Da (predicted); P37088-4: 73603 Da (predicted); P37088-5: 77980 Da (predicted); P37088-6: 78234 Da (predicted).
Ubiquitinated. Can be ubiquitinated at multiple sites and undergo monoubiquitination and polyubiquitination. Ubiquitination by NEDD4 or NEDD4L inhibits the ENaC channel through endocytosis, intracellular retention and degradation of its individual subunits;ENaC is activated through the proteolytic maturation of its subunits. Furin cleaves the SCNN1A subunit, which results in a stepwise increase in the open probability of the channel due to the release of an inhibitory tract (By similarity). BPIFA1, which is recruited by the SCNN1B subunit, prevents the proteolytic activation of ENaC (PubMed:24124190);N-glycosylated

Subunit：Heterotrimer; containing an alpha/SCNN1A, a beta/SCNN1B and a gamma/SCNN1G subunit (PubMed:30251954, PubMed:32729833). Interacts with WWP1 (via WW domains) (PubMed:9169421). Interacts with WWP2 (via WW domains); inhibits the channel (PubMed:12167593, PubMed:9169421). Interacts with BPIFA1; the interaction is indirect via SCNN1B and inhibits the proteolytic processing of SCNN1A and SCNN1G and the activation of ENaC (PubMed:24124190). Interacts with the full-length immature form of PCSK9 (pro-PCSK9); inhibits ENaC by promoting its proteasomal degradation (PubMed:22493497)

RRID

AB_3103273

Database

Entrez Gene: 6337 Human ; 20276 Mouse ; 25122 Rat

SwissProt: P37088 Human ; Q61180 Mouse ;

OMIM: 264350 Human

Synonyms

Alpha ENaC 2; Alpha ENaC; Alpha NaCH; Alpha-NaCH; Amiloride sensitive epithelial sodium channel alpha subunit; Amiloride sensitive sodium channel subunit alpha; Amiloride-sensitive sodium channel subunit alpha; ENaCa; ENaCalpha; Epithelial Na(+) channel subunit alpha; Epithelial Na+ channel subunit alpha; FLJ21883; Nonvoltage gated sodium channel 1 subunit alpha; Nonvoltage-gated sodium channel 1 subunit alpha; SCNEA; SCNN 1; SCNN1; SCNN1A; SCNNA_HUMAN; Sodium channel nonvoltage gated 1 alpha.

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Alpha-ENaC Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Country or Region *

Email Address *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Alpha-ENaC Antibody

Alpha-ENaC Antibody Featured Recommendations:

Product Detail

Background

Documentation

Alpha-ENaC Antibody Related Classifications

Your Recently Viewed Products:

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report