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Wnt10a Antibody

(Synonyms: FLJ14301; OODD; Protein Wnt-10a ; SSPS; STHAG4; Wingless type MMTV integration site family member 10A antibody; WN10A_HUMAN; wnt10a; )
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Based on 1 Customer Validation

Wnt10a Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Wnt10a.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ELISA

  • Reactivity :

    Human, Mouse, Goat

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Wnt10a Antibody
  • Wnt10a Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-2000 1:5000-20000

Product Details

Description

Wnt10a Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Wnt10a.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Goat
  • Observed Molecular Weight
    Observed band size: 40 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 46 kDa
Species Reactivity Database
Immunogen

Synthesized peptide derived from human protein AA range: 110-190

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103289

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Wnt10a Antibody
    Western blot analysis of extracts from RAW264.7(lane 2(20ug) and RAW264.7(lane 3(40ug) using Wnt10a Antibody (HY-P81237) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Wnt10a is a Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt/beta-catenin signaling pathway. Plays a role in normal ectoderm development. Required for normal tooth development. Required for normal postnatal development and maintenance of tongue papillae and sweat ducts. Required for normal proliferation of basal cells in tongue filiform papillae, plantar epithelium and sweat ducts. Required for normal expression of keratins in tongue papillae. Required for normal expression of KRT9 in foot plant epithelium. Required for normal hair follicle function[1][2][3].

  • Subcellular Localization

    Secreted, extracellular space, extracellular matrix; Secreted

  • Subunit

    Forms a soluble 1:1 complex with AFM; this prevents oligomerization and is required for prolonged biological activity (PubMed:26902720). The complex with AFM may represent the physiological form in body fluids (PubMed:26902720)

  • SwissProt ID

    Q9GZT5

  • Gene ID
    80326 [NCBI]
  • Synonyms

    FLJ14301; OODD; Protein Wnt-10a ; SSPS; STHAG4; Wingless type MMTV integration site family member 10A antibody; WN10A_HUMAN; wnt10a;

  • Research Field

    Stem Cells; Signaling Pathways; Wnt Secreted;Cancer

[1]. Adaimy L, et al. Mutation in WNT10A is associated with an autosomal recessive ectodermal dysplasia: the odonto-onycho-dermal dysplasia. Am J Hum Genet. 2007 Oct;81(4):821-8. [Content Brief]

[2]. Xu M, et al. WNT10A mutation causes ectodermal dysplasia by impairing progenitor cell proliferation and KLF4-mediated differentiation. Nat Commun. 2017 Jun 7;8:15397. [Content Brief]

[3]. Yuan Q, et al. Role of WNT10A in failure of tooth development in humans and zebrafish. Mol Genet Genomic Med. 2017 Nov;5(6):730-741. [Content Brief]

Wnt10a Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from RAW264.7(lane 2(20ug) and RAW264.7(lane 3(40ug) using Wnt10a Antibody (HY-P81237) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Wnt10a Antibody Product Image
    1/16
  • Experimental Validation Results for Wnt10a Antibody
  • Wnt10a Antibody Product Image
  • Experimental Validation Results for Wnt10a Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from RAW264.7(lane 2(20ug) and RAW264.7(lane 3(40ug) using Wnt10a Antibody (HY-P81237) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Wnt10a Antibody
  • Wnt10a Antibody Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from RAW264.7(lane 2(20ug) and RAW264.7(lane 3(40ug) using Wnt10a Antibody (HY-P81237) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Wnt10a Antibody Product Image WB