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TCP 1 alpha Antibody (YA2238)

(Synonyms: TCP1; CCT1; CCTA; T-complex protein 1 subunit alpha; TCP-1-alpha; CCT-alpha)
Customer Review

Based on 1 Customer Validation

TCP 1 alpha Antibody (YA2238) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TCP 1 alpha.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in rabbit IgG in 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
  • TCP 1 alpha Antibody (YA2238) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:2000 1:100-1:200 1:50-1:200 1:50

Product Details

Description

TCP 1 alpha Antibody (YA2238) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TCP 1 alpha.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 60 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 60 kDa
Species Reactivity Database

Entrez Gene: 6950 Human

SwissProt: P17987 Human

Immunogen

A synthesized peptide derived from human TCP1 alpha aa1-55/556.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in rabbit IgG in 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), Daudi (lane 2, 15 μg), HT-1080 (lane 3, 15 μg), HepG2 (lane 4, 15 μg), Ramos (lane 5, 15 μg), and Jurkat (lane 6, 15 μg) using TCP 1 alpha Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
    Immunohistochemical analysis of paraffin-embedded human lungs tissue using TCP 1 alpha antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82493, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
    Immunohistochemical analysis of paraffin-embedded human appendix‌ tissue using TCP 1 alpha antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82493, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
    Immunohistochemical analysis of paraffin-embedded human Liver tissue using TCP 1 alpha antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82493, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
    Immunohistochemical analysis of paraffin-embedded human thyroid tissue using TCP 1 alpha antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82493, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using TCP 1 alpha antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82493, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
    Immunohistochemical analysis of paraffin-embedded human Colorectal cancer tissue using TCP 1 alpha antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82493, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
    Immunocytochemistry analysis of Hela cells labeling TCP 1 alpha with TCP 1 alpha Antibody (HY-P82493) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with TCP 1 alpha Antibody (HY-P82493) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
    Immunocytochemistry analysis of Hela cells labeling TCP 1 alpha with TCP 1 alpha Antibody (HY-P82493) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with TCP 1 alpha Antibody (HY-P82493) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    TCP 1 alpha component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia[1][2][3][4][5].

  • Subcellular Localization

    Cytoplasm, cytosol; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

  • Subunit

    Component of the chaperonin-containing T-complex (TRiC), a hexadecamer composed of two identical back-to-back stacked rings enclosing a protein folding chamber (PubMed:1630492, PubMed:20080638, PubMed:25467444, PubMed:36493755, PubMed:35449234, PubMed:37193829). Each ring is made up of eight different subunits: TCP1/CCT1, CCT2, CCT3, CCT4, CCT5, CCT6A/CCT6, CCT7, CCT8 (PubMed:36493755, PubMed:35449234, PubMed:37193829). Interacts with PACRG (PubMed:14532270). Interacts with GBA1 (PubMed:21098288). Interacts with DLEC1 (PubMed:33144677)

  • SwissProt ID

    P17987

  • Gene ID
    6950 [NCBI]
  • Synonyms

    TCP1; CCT1; CCTA; T-complex protein 1 subunit alpha; TCP-1-alpha; CCT-alpha

  • Research Field

    Signal Transduction

References

[1]. Freund A, et al. Proteostatic control of telomerase function through TRiC-mediated folding of TCAB1. Cell. 2014 Dec 4;159(6):1389-403. [Content Brief]

[2]. Gestaut D, et al. Structural visualization of the tubulin folding pathway directed by human chaperonin TRiC/CCT. Cell. 2022 Dec 8;185(25):4770-4787.e20. [Content Brief]

[3]. Kelly JJ, et al. Snapshots of actin and tubulin folding inside the TRiC chaperonin. Nat Struct Mol Biol. 2022 May;29(5):420-429. [Content Brief]

[4]. Liu C, et al. Pathway and mechanism of tubulin folding mediated by TRiC/CCT along its ATPase cycle revealed using cryo-EM. Commun Biol. 2023 May 16;6(1):531. [Content Brief]

[5]. Seo S, et al. BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly. Proc Natl Acad Sci U S A. 2010 Jan 26;107(4):1488-93. [Content Brief]

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TCP 1 alpha Antibody (YA2238) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), Daudi (lane 2, 15 μg), HT-1080 (lane 3, 15 μg), HepG2 (lane 4, 15 μg), Ramos (lane 5, 15 μg), and Jurkat (lane 6, 15 μg) using TCP 1 alpha Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lungs tissue using TCP 1 alpha antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82493, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human appendix‌ tissue using TCP 1 alpha antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82493, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Liver tissue using TCP 1 alpha antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82493, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human thyroid tissue using TCP 1 alpha antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82493, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using TCP 1 alpha antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82493, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colorectal cancer tissue using TCP 1 alpha antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82493, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling TCP 1 alpha with TCP 1 alpha Antibody (HY-P82493) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with TCP 1 alpha Antibody (HY-P82493) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling TCP 1 alpha with TCP 1 alpha Antibody (HY-P82493) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with TCP 1 alpha Antibody (HY-P82493) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • TCP 1 alpha Antibody (YA2238) Product Image
    1/16
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
  • TCP 1 alpha Antibody (YA2238) Product Image
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), Daudi (lane 2, 15 μg), HT-1080 (lane 3, 15 μg), HepG2 (lane 4, 15 μg), Ramos (lane 5, 15 μg), and Jurkat (lane 6, 15 μg) using TCP 1 alpha Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lungs tissue using TCP 1 alpha antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82493, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human appendix‌ tissue using TCP 1 alpha antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82493, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Liver tissue using TCP 1 alpha antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82493, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human thyroid tissue using TCP 1 alpha antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82493, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using TCP 1 alpha antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82493, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colorectal cancer tissue using TCP 1 alpha antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82493, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling TCP 1 alpha with TCP 1 alpha Antibody (HY-P82493) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with TCP 1 alpha Antibody (HY-P82493) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling TCP 1 alpha with TCP 1 alpha Antibody (HY-P82493) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with TCP 1 alpha Antibody (HY-P82493) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
  • Experimental Validation Results for TCP 1 alpha Antibody (YA2238)
  • TCP 1 alpha Antibody (YA2238) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), Daudi (lane 2, 15 μg), HT-1080 (lane 3, 15 μg), HepG2 (lane 4, 15 μg), Ramos (lane 5, 15 μg), and Jurkat (lane 6, 15 μg) using TCP 1 alpha Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • TCP 1 alpha Antibody (YA2238) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lungs tissue using TCP 1 alpha antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82493, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • TCP 1 alpha Antibody (YA2238) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human appendix‌ tissue using TCP 1 alpha antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82493, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • TCP 1 alpha Antibody (YA2238) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Liver tissue using TCP 1 alpha antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82493, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • TCP 1 alpha Antibody (YA2238) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human thyroid tissue using TCP 1 alpha antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82493, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • TCP 1 alpha Antibody (YA2238) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using TCP 1 alpha antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82493, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • TCP 1 alpha Antibody (YA2238) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Colorectal cancer tissue using TCP 1 alpha antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82493, 1:600 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • TCP 1 alpha Antibody (YA2238) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling TCP 1 alpha with TCP 1 alpha Antibody (HY-P82493) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with TCP 1 alpha Antibody (HY-P82493) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • TCP 1 alpha Antibody (YA2238) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling TCP 1 alpha with TCP 1 alpha Antibody (HY-P82493) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with TCP 1 alpha Antibody (HY-P82493) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • TCP 1 alpha Antibody (YA2238) Product Image WB
  • TCP 1 alpha Antibody (YA2238) Product Image IHC-P
  • TCP 1 alpha Antibody (YA2238) Product Image IHC-P
  • TCP 1 alpha Antibody (YA2238) Product Image IHC-P
  • TCP 1 alpha Antibody (YA2238) Product Image IHC-P
  • TCP 1 alpha Antibody (YA2238) Product Image IHC-P
  • TCP 1 alpha Antibody (YA2238) Product Image IHC-P
  • TCP 1 alpha Antibody (YA2238) Product Image ICC
  • TCP 1 alpha Antibody (YA2238) Product Image ICC