Angiopoietin Like 4 Antibody (YA2090)(PBS only)

Cat. No.: HY-P82345A: Data Sheet User Guide for Antibodies
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Angiopoietin Like 4 Antibody (YA2090) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Angiopoietin Like 4.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Angiopoietin Like 4 Antibody (YA2090) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Angiopoietin Like 4.

Host

Rabbit

Molecular Weight

Predicted band size: 45 kDa;

Observed band size: 55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9BY76

Gene ID

51129 [NCBI]

Immunogen

Recombinant protein of human Angiopoietin-like 4

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:100
FC FC: Flow Cytometry	1:200-1:1000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Mediates inactivation of the lipoprotein lipase LPL, and thereby plays a role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism (PubMed:19270337, PubMed:21398697, PubMed:27929370, PubMed:29899144). May also play a role in regulating glucose homeostasis and insulin sensitivity (Probable). Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage (PubMed:14583458, PubMed:17068295). Upon heterologous expression, inhibits the adhesion of endothelial cell to the extracellular matrix (ECM), and inhibits the reorganization of the actin cytoskeleton, formation of actin stress fibers and focal adhesions in endothelial cells that have adhered to ANGPTL4-containing ECM (in vitro) (PubMed:17068295). Depending on context, may modulate tumor-related angiogenesis (By similarity); Mediates inactivation of the lipoprotein lipase LPL, and thereby plays an important role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism (PubMed:19270337, PubMed:21398697, PubMed:27929370, PubMed:29899144). Has higher activity in LPL inactivation than the uncleaved protein (PubMed:19270337, PubMed:21398697)

Subcellular Localization：Secreted; Secreted, extracellular space, extracellular matrix

Expression：
Tissue_specificity:Detected in blood plasma (at protein level) (PubMed:29899519) . Detected in liver (PubMed:10698685) . Detected in white fat tissue and placenta (PubMed:10866690) . Expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney

Induction:Up-regulated when cells are exposed to severe hypoxia (in vitro)

Isoforms & Post-Translational Modification：Q9BY76 has 3 isomers: Q9BY76-1: 45214 Da (predicted); Q9BY76-2: 40917 Da (predicted); Q9BY76-3: 26853 Da (predicted).
N-glycosylated;Forms disulfide-linked dimers and tetramers;Cleaved into a smaller N-terminal chain and a larger chain that contains the fibrinogen C-terminal domain; both cleaved and uncleaved forms are detected in the extracellular space. The cleaved form is not present within the cell

Subunit：Homooligomer; disulfide-linked via Cys residues in the N-terminal part of the protein (PubMed:19270337). The homooligomer undergoes proteolytic processing to release the ANGPTL4 C-terminal chain, which circulates as a monomer (PubMed:19270337). The homooligomer unprocessed form is able to interact with the extracellular matrix (PubMed:21398697)

Research Field

Cardiovascular

Synonyms

NL2; ARP4; FIAF; HARP; PGAR; HFARP; TGQTL; UNQ171; pp1158

Documentation

User Guide for Antibodies (1077 KB)