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Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455)

(Synonyms: ERK1, PRKM3, MAPK3, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, Microtubule-associated protein 2 kinase, p44-ERK1, ERK-1, p44-MAPK)
Customer Review

Based on 1 Customer Validation

Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, FC, IF-Tissue

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC FC
  • Experimental Validation Results for Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455)
  • Experimental Validation Results for Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455)
  • Experimental Validation Results for Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455)
  • Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:5000-1:10000 1:100 1:1000 1:200 1:1000 Use at an assay dependent concentration.

Product Details

Description

Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 41/43 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 41/43 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human.AA range:166-215.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

RRID

AB_3102477

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455)
    Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg)，NIH/3T3 (lane 4(20μg) ) and NIH/3T3 (lane 5(40μg) )using Phospho-ERK1/2 (HY-P80392) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80392, 1/1000) and Loading control antibody (ERK2, HY-P80394, 1/1000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455)
    Immunocytochemistry analysis of Hela cells labeling Phospho-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187)withPhospho-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187) antibody (HY-P80392) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187) antibody (HY-P80392) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455)
    Flow cytometric analysis of 1X10^6 Hela cells labeling Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody(HY-P80392, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1μg/mL dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    ERK1/2 / is a P27361: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors.
    P28482: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors.
    [1].

  • Subcellular Localization

    P27361: Cytoplasm; Nucleus; Membrane, caveola; Cell junction, focal adhesion
    P28482: Cytoplasm, cytoskeleton, spindle; Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm; Membrane, caveola; Cell junction, focal adhesion

  • Subunit

    P27361: Binds both upstream activators and downstream substrates in multimolecular complexes. Found in a complex with at least BRAF, HRAS, MAP2K1/MEK1, MAPK3 and RGS14 (By similarity).
    P28482: Binds both upstream activators and downstream substrates in multimolecular complexes. This interaction inhibits its tyrosine-kinase activity.

  • SwissProt ID

    P27361 / P28482

  • Gene ID
    5595 [NCBI] / 5594 [NCBI]
  • Synonyms

    ERK1, PRKM3, MAPK3, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, Microtubule-associated protein 2 kinase, p44-ERK1, ERK-1, p44-MAPK

  • Research Field

    Cell Biology

References

[1]. Zhu G, et al. TRIM15 and CYLD regulate ERK activation via lysine-63-linked polyubiquitination. Nat Cell Biol. 2021 Sep;23(9):978-991. [Content Brief]

Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg)，NIH/3T3 (lane 4(20μg) ) and NIH/3T3 (lane 5(40μg) )using Phospho-ERK1/2 (HY-P80392) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80392, 1/1000) and Loading control antibody (ERK2, HY-P80394, 1/1000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Phospho-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187)withPhospho-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187) antibody (HY-P80392) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187) antibody (HY-P80392) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody(HY-P80392, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1μg/mL dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455) Product Image
    1/16
  • Experimental Validation Results for Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455)
  • Experimental Validation Results for Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455)
  • Experimental Validation Results for Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455)
  • Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455) Product Image
  • Experimental Validation Results for Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg)，NIH/3T3 (lane 4(20μg) ) and NIH/3T3 (lane 5(40μg) )using Phospho-ERK1/2 (HY-P80392) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80392, 1/1000) and Loading control antibody (ERK2, HY-P80394, 1/1000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Phospho-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187)withPhospho-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187) antibody (HY-P80392) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187) antibody (HY-P80392) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455)
    1/1
    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody(HY-P80392, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1μg/mL dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455)
  • Experimental Validation Results for Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455)
  • Experimental Validation Results for Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455)
  • Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg)，NIH/3T3 (lane 4(20μg) ) and NIH/3T3 (lane 5(40μg) )using Phospho-ERK1/2 (HY-P80392) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80392, 1/1000) and Loading control antibody (ERK2, HY-P80394, 1/1000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Phospho-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187)withPhospho-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187) antibody (HY-P80392) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187) antibody (HY-P80392) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455) Product Image
    MedchemExpress Validation

    Flow cytometric analysis of 1X10^6 Hela cells labeling Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody(HY-P80392, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1μg/mL dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455) Product Image WB
  • Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455) Product Image ICC
  • Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455) Product Image FC