Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455)

Cat. No.: HY-P80392: Data Sheet COA
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Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187).

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사이즈	재고	수량
20 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
제품 설명

제품 설명

Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187).

Host

Rabbit

Clonality

Recombinant,Monoclonal

분자량

Predicted band size: 41/43 kDa;

Observed band size: 41/43 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P27361 / P28482

Gene ID

5595 [NCBI] / 5594 [NCBI]

Immunogen

Synthetic peptide corresponding to Human.AA range:166-215.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:5000-1:10000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:1000
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:200
FC FC: Flow Cytometry	1:1000
IP IP: Immunoprecipitation	Use at an assay dependent concentration.

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Phosphorylated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

ALL WB ICC FC

Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg)，NIH/3T3 (lane 4(20μg) ) and NIH/3T3 (lane 5(40μg) )using Phospho-ERK1/2 (HY-P80392) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80392, 1/1000) and Loading control antibody (ERK2, HY-P80394, 1/1000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of Hela cells labeling Phospho-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187)withPhospho-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187) antibody (HY-P80392) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187) antibody (HY-P80392) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Flow cytometric analysis of 1X10^6 Hela cells labeling Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody(HY-P80392, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1μg/mL dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

Function：P27361: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway (PubMed:34497368). MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors.
P28482: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors.

Subcellular Localization：P27361: Cytoplasm; Nucleus; Membrane, caveola; Cell junction, focal adhesion
P28482: Cytoplasm, cytoskeleton, spindle; Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm; Membrane, caveola; Cell junction, focal adhesion

Subunit：P27361: Binds both upstream activators and downstream substrates in multimolecular complexes. Found in a complex with at least BRAF, HRAS, MAP2K1/MEK1, MAPK3 and RGS14 (By similarity).
P28482: Binds both upstream activators and downstream substrates in multimolecular complexes. This interaction inhibits its tyrosine-kinase activity.

RRID

AB_3102477

Database

Entrez Gene: 5595 Human ; 26413 Mouse ; 50689 Rat

SwissProt: P27361 Human ; P28482 Human ; P63085 Mouse ; Q63844 Mouse ; P21708 Rat ; P63086 Rat

OMIM: 601795 Human

Research Field

Cell Biology

각종 서류

Select Batch:

Data Sheet (236 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455)

Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455) Featured Recommendations:

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Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) Antibody (YA455) Related Classifications

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