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Alkaline Phosphatase(For 4 isoform) Antibody

(Synonyms: ALPP; PLAP; Alkaline phosphatase; placental type; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1)
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Based on 1 Customer Validation

Alkaline Phosphatase(For 4 isoform) Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Alkaline Phosphatase(For 4 isoform).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS (pH7.4), 0.2% BSA, 40% Glycerol, 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Alkaline Phosphatase(For 4 isoform) Antibody
  • Alkaline Phosphatase(For 4 isoform) Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:100-5000

Product Details

Description

Alkaline Phosphatase(For 4 isoform) Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Alkaline Phosphatase(For 4 isoform).

  • Host Rabbit
  • Species Reactivity
    Human
  • Calculated Molecular Weight Predicted band size: 58 kDa
Immunogen

Synthetic peptide within Human Alkaline Phosphatase aa 314-363 / 535.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.2% BSA, 40% Glycerol, 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Alkaline Phosphatase(For 4 isoform) Antibody
    Western blot analysis of extracts from Hela (lane 2, 20ug) and Hela (lane 3, 40ug) using Alkaline Phosphatase(For 4 isoform) Antibody (HY-P810649). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Alkaline Phosphatase is an Alkaline phosphatase that can hydrolyze various phosphate compounds

  • Subcellular Localization

    Cell membrane; Lipid-anchor, GPI-anchor

  • Expression


    Tissue_specificity:Protein levels were detected in the placenta.

  • Subunit

    Homodimer

  • SwissProt ID

    P05187

  • Gene ID
    250 [NCBI]
  • Synonyms

    ALPP; PLAP; Alkaline phosphatase; placental type; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1

Alkaline Phosphatase(For 4 isoform) Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2, 20ug) and Hela (lane 3, 40ug) using Alkaline Phosphatase(For 4 isoform) Antibody (HY-P810649). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Alkaline Phosphatase(For 4 isoform) Antibody Product Image
    1/16
  • Experimental Validation Results for Alkaline Phosphatase(For 4 isoform) Antibody
  • Alkaline Phosphatase(For 4 isoform) Antibody Product Image
  • Experimental Validation Results for Alkaline Phosphatase(For 4 isoform) Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2, 20ug) and Hela (lane 3, 40ug) using Alkaline Phosphatase(For 4 isoform) Antibody (HY-P810649). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Alkaline Phosphatase(For 4 isoform) Antibody
  • Alkaline Phosphatase(For 4 isoform) Antibody Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2, 20ug) and Hela (lane 3, 40ug) using Alkaline Phosphatase(For 4 isoform) Antibody (HY-P810649). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Alkaline Phosphatase(For 4 isoform) Antibody Product Image WB