Granzyme B Antibody (YA1326)

Cat. No.: HY-P81581: Data Sheet COA
SDS
User Guide for Antibodies
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Technical Support

Granzyme B Antibody (YA1326) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Granzyme B.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Granzyme B Antibody (YA1326) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Granzyme B Antibody (YA1326) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Granzyme B.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Observed band size: 32 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P10144

Gene ID

3002 [NCBI]

Immunogen

Amino acids 1-247 representing full length granzyme B of human origin.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:100-1:1000
IP IP: Immunoprecipitation	1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-500
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-500

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

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ALL WB IHC-P

Western blot analysis of GZMB (lane 2(5ng) and GZMB (lane 3(10ng)using Granzyme B (HY-P81581) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P81581, 1/1000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue using Granzyme B Antibody (YA1326). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81581, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue using Granzyme B Antibody (YA1326). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81581, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed:1985927, PubMed:3262682, PubMed:3263427). It cleaves after Asp (PubMed:1985927, PubMed:8258716). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death (PubMed:31953257, PubMed:32188940). Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -9 and -10 (CASP3, CASP9 and CASP10, respectively) to give rise to active enzymes mediating apoptosis (PubMed:9852092). Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (By similarity)

Subcellular Localization：Secreted; Cytolytic granule

Expression：
Induction:By staphylococcal enterotoxin A (SEA) in peripheral blood leukocytes

RRID

AB_3103693

Database

Entrez Gene: 3002 Human ; 14939 Mouse ; 171528 Rat

SwissProt: P10144 Human ; P04187 Mouse ; P18291 Rat

OMIM: 123910 Human

Research Field

Immunology

Synonyms

CGL1, CSPB, CTLA1,GRB

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Granzyme B Antibody (YA1326) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Granzyme B Antibody (YA1326)

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