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Granzyme B Antibody (YA1326)

(Synonyms: CGL1, CSPB, CTLA1,GRB)
Customer Review

Based on 1 Customer Validation

Granzyme B Antibody (YA1326) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Granzyme B.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IP, ICC/IF, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Granzyme B Antibody (YA1326)
  • Experimental Validation Results for Granzyme B Antibody (YA1326)
  • Experimental Validation Results for Granzyme B Antibody (YA1326)
  • Granzyme B Antibody (YA1326) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:100-1:1000 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) 1:50-500 1:50-500

Product Details

Description

Granzyme B Antibody (YA1326) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Granzyme B.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 32 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity Database
Immunogen

Amino acids 1-247 representing full length granzyme B of human origin.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3103693

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Granzyme B Antibody (YA1326)
    Western blot analysis of GZMB (lane 2(5ng) and GZMB (lane 3(10ng)using Granzyme B (HY-P81581) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P81581, 1/1000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Granzyme B Antibody (YA1326)
    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue using Granzyme B Antibody (YA1326). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81581, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Granzyme B Antibody (YA1326)
    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue using Granzyme B Antibody (YA1326). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81581, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Granzyme B is an Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -9 and -10 (CASP3, CASP9 and CASP10, respectively) to give rise to active enzymes mediating apoptosis. Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair[1][2][3][4][5][6][7].

  • Subcellular Localization

    Secreted; Cytolytic granule

  • Expression


    Induction:By staphylococcal enterotoxin A (SEA) in peripheral blood leukocytes

  • SwissProt ID

    P10144

  • Gene ID
    3002 [NCBI]
  • Synonyms

    CGL1, CSPB, CTLA1,GRB

  • Research Field

    Immunology

Granzyme B Antibody (YA1326) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of GZMB (lane 2(5ng) and GZMB (lane 3(10ng)using Granzyme B (HY-P81581) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P81581, 1/1000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat spleen tissue using Granzyme B Antibody (YA1326). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81581, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat spleen tissue using Granzyme B Antibody (YA1326). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81581, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Granzyme B Antibody (YA1326) Product Image
    1/16
  • Experimental Validation Results for Granzyme B Antibody (YA1326)
  • Experimental Validation Results for Granzyme B Antibody (YA1326)
  • Experimental Validation Results for Granzyme B Antibody (YA1326)
  • Granzyme B Antibody (YA1326) Product Image
  • Experimental Validation Results for Granzyme B Antibody (YA1326)
    1/1
    MedchemExpress Validation
     Western blot analysis of GZMB (lane 2(5ng) and GZMB (lane 3(10ng)using Granzyme B (HY-P81581) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P81581, 1/1000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Granzyme B Antibody (YA1326)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat spleen tissue using Granzyme B Antibody (YA1326). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81581, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Granzyme B Antibody (YA1326)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat spleen tissue using Granzyme B Antibody (YA1326). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81581, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Granzyme B Antibody (YA1326)
  • Experimental Validation Results for Granzyme B Antibody (YA1326)
  • Experimental Validation Results for Granzyme B Antibody (YA1326)
  • Granzyme B Antibody (YA1326) Product Image
    MedchemExpress Validation

    Western blot analysis of GZMB (lane 2(5ng) and GZMB (lane 3(10ng)using Granzyme B (HY-P81581) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P81581, 1/1000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Granzyme B Antibody (YA1326) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue using Granzyme B Antibody (YA1326). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81581, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Granzyme B Antibody (YA1326) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue using Granzyme B Antibody (YA1326). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81581, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Granzyme B Antibody (YA1326) Product Image WB
  • Granzyme B Antibody (YA1326) Product Image IHC-P
  • Granzyme B Antibody (YA1326) Product Image IHC-P