Bcl-2 Antibody (YA6404)

Cat. No.: HY-P86712: Data Sheet User Guide for Antibodies
FAQs
Technical Support

Bcl-2 Antibody (YA6404) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to Bcl-2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Bcl-2 Antibody (YA6404) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to Bcl-2.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 26 kDa

Species Reactivity

Human

SwissProt ID

P10415

Gene ID

596 [NCBI]

Immunogen

Recombinant human Bcl-2 protein aa 1-211/239

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200
FC FC: Flow Cytometry	1μg/Test

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed:11368354). Appears to function in a feedback loop system with caspases (PubMed:11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed:11368354). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed:18570871, PubMed:20889974, PubMed:21358617). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785)

Subcellular Localization：Mitochondrion outer membrane,Nucleus membrane,Endoplasmic reticulum membrane,Cytoplasm

Expression：
Tissue_Specificity: Expressed in a variety of tissues

Isoforms & Post-Translational Modification：P10415 has two isomers: P10415-1: 26266 Da (predicted); P10415-2: 22337 Da (predicted).
Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity (PubMed:11368354)丨Proteolytically cleaved by caspases during apoptosis丨Monoubiquitinated by PRKN, leading to an increase in its stability (PubMed:20889974)

Subunit：Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L)

Synonyms

Apoptosis regulator Bcl 2; Apoptosis regulator Bcl2; AW986256; B cell CLL/lymphoma 2; B cell leukemia/lymphoma 2; B cell lymphoma 2; Bcl 2; Bcl-2; Bcl2; BCL2 protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; Leukemia/lymphoma, B-cell, 2; Oncogene B-cell leukemia 2; BCL2_HUMAN.

Documentation

User Guide for Antibodies (1077 KB)