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INMT Antibody (YA9990)

(Synonyms: Indolethylamine N-methyltransferase, Indolamine N-methyltransferase, Aromatic alkylamine N-methyltransferase, Thioether S-methyltransferase, Amine N-methyltransferase, Arylamine N-methyltransferase, TEMT, INMT)

INMT Antibody (YA9990) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to INMT.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH 7.4), containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for INMT Antibody (YA9990)
  • INMT Antibody (YA9990) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000-2000 1:50-200

Product Details

Description

INMT Antibody (YA9990) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to INMT.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 25 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 28 kDa
Immunogen

Synthetic peptide corresponding to human INMT.

Sensitivity

Endogenous

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH 7.4), containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for INMT Antibody (YA9990)
    Western blot analysis was performed on protein extracts (25 μg) from Mouse lung (lane 2), Mouse liver (lane 3), Mouse kidney (lane 4), and Rat liver (lane 5) using INMT antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    INMT functions as a thioether S-methyltransferase and is active with a variety of thioethers and the corresponding selenium and tellurium compounds, including 3-methylthiopropionaldehyde, dimethyl selenide, dimethyl telluride, 2-methylthioethylamine, 2-methylthioethanol, methyl-n-propyl sulfide and diethyl sulfide. Plays an important role in the detoxification of selenium compounds. Catalyzes the N-methylation of tryptamine and structurally related compounds

  • Subcellular Localization

    Cytoplasm

  • Expression


    Tissue_specificity:Widely expressed. The highest levels were in thyroid, adrenal gland, human lung. Intermediate levels in heart, placenta, skeletal muscle, testis, small intestine, pancreas, stomach, spinal cord, lymph node and trachea. Very low levels in human kidney and liver, in human spleen, thymus, ovary, colon and bone marrow. Not expressed in peripheral blood leukocytes and brain

  • Isoforms & Post-Translational Modification

    O95050 has 2 isomers: O95050-1: 28891 Da (predicted); O95050-2: 28834 Da (predicted).

  • Subunit

    Monomer

  • SwissProt ID

    O95050

  • Gene ID
    11185 [NCBI]
  • Synonyms

    Indolethylamine N-methyltransferase, Indolamine N-methyltransferase, Aromatic alkylamine N-methyltransferase, Thioether S-methyltransferase, Amine N-methyltransferase, Arylamine N-methyltransferase, TEMT, INMT

INMT Antibody (YA9990) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Mouse lung (lane 2), Mouse liver (lane 3), Mouse kidney (lane 4), and Rat liver (lane 5) using INMT antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • INMT Antibody (YA9990) Product Image
    1/16
  • Experimental Validation Results for INMT Antibody (YA9990)
  • INMT Antibody (YA9990) Product Image
  • Experimental Validation Results for INMT Antibody (YA9990)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Mouse lung (lane 2), Mouse liver (lane 3), Mouse kidney (lane 4), and Rat liver (lane 5) using INMT antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for INMT Antibody (YA9990)
  • INMT Antibody (YA9990) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from Mouse lung (lane 2), Mouse liver (lane 3), Mouse kidney (lane 4), and Rat liver (lane 5) using INMT antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • INMT Antibody (YA9990) Product Image WB