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Toll-Like Receptor 5 Antibody (YA3265)

(Synonyms: Toll-Like Receptor 5; SLEB1; TIL3; Tlr5; Toll like receptor 5 precursor)
Customer Review

Based on 1 Customer Validation

Toll-Like Receptor 5 Antibody (YA3265) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Toll-Like Receptor 5.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Toll-Like Receptor 5 Antibody (YA3265)
  • Experimental Validation Results for Toll-Like Receptor 5 Antibody (YA3265)
  • Toll-Like Receptor 5 Antibody (YA3265) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Toll-Like Receptor 5 Antibody (YA3265) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Toll-Like Receptor 5.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Calculated Molecular Weight Predicted band size: 98 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human TLR5 aa250-410.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Toll-Like Receptor 5 Antibody (YA3265)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), A549 (lane 2, 15 μg), HT-29 (lane 3, 15 μg), RAW264.7 (lane 4, 15 μg), and HaCaT (lane 5, 15 μg) using Toll-Like Receptor 5 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Toll-Like Receptor 5 Antibody (YA3265)
    Western blot analysis of extracts from Hela (lane 2(40μg),SK-Br-3 (lane 3(40μg), A549 (lane 4(40μg),U-251 (lane 5(40μg)using Toll-Like Receptor 5 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Toll-Like Receptor 5 is a Pattern recognition receptor (PRR) located on the cell surface that participates in the activation of innate immunity and inflammatory response. Recognizes small molecular motifs named pathogen-associated molecular pattern (PAMPs) expressed by pathogens and microbe-associated molecular patterns (MAMPs) usually expressed by resident microbiota. Upon ligand binding such as bacterial flagellins, recruits intracellular adapter proteins MYD88 and TRIF leading to NF-kappa-B activation, cytokine secretion and induction of the inflammatory response. Plays thereby an important role in the relationship between the intestinal epithelium and enteric microbes and contributes to the gut microbiota composition throughout life[1][2][3][4][5].

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:Highly expressed on the basolateral surface of intestinal epithelia (PubMed:11489966) . Expressed also in other cells such as lung epithelial cells (PubMed:11489966, PubMed:18490781)

  • Subunit

    Homodimer (PubMed:22173220). Interacts with MYD88 (via TIR domain) (PubMed:20855887). Interacts with TICAM1 (via TIR domain) (PubMed:20855887).

  • SwissProt ID

    O60602

  • Gene ID
    7100 [NCBI]
  • Synonyms

    Toll-Like Receptor 5; SLEB1; TIL3; Tlr5; Toll like receptor 5 precursor

  • Research Field

    Immunology

Toll-Like Receptor 5 Antibody (YA3265) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), A549 (lane 2, 15 μg), HT-29 (lane 3, 15 μg), RAW264.7 (lane 4, 15 μg), and HaCaT (lane 5, 15 μg) using Toll-Like Receptor 5 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(40μg),SK-Br-3 (lane 3(40μg), A549 (lane 4(40μg),U-251 (lane 5(40μg)using Toll-Like Receptor 5 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Toll-Like Receptor 5 Antibody (YA3265) Product Image
    1/16
  • Experimental Validation Results for Toll-Like Receptor 5 Antibody (YA3265)
  • Experimental Validation Results for Toll-Like Receptor 5 Antibody (YA3265)
  • Toll-Like Receptor 5 Antibody (YA3265) Product Image
  • Experimental Validation Results for Toll-Like Receptor 5 Antibody (YA3265)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), A549 (lane 2, 15 μg), HT-29 (lane 3, 15 μg), RAW264.7 (lane 4, 15 μg), and HaCaT (lane 5, 15 μg) using Toll-Like Receptor 5 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Toll-Like Receptor 5 Antibody (YA3265)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(40μg),SK-Br-3 (lane 3(40μg), A549 (lane 4(40μg),U-251 (lane 5(40μg)using Toll-Like Receptor 5 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Toll-Like Receptor 5 Antibody (YA3265)
  • Experimental Validation Results for Toll-Like Receptor 5 Antibody (YA3265)
  • Toll-Like Receptor 5 Antibody (YA3265) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), A549 (lane 2, 15 μg), HT-29 (lane 3, 15 μg), RAW264.7 (lane 4, 15 μg), and HaCaT (lane 5, 15 μg) using Toll-Like Receptor 5 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Toll-Like Receptor 5 Antibody (YA3265) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(40μg),SK-Br-3 (lane 3(40μg), A549 (lane 4(40μg),U-251 (lane 5(40μg)using Toll-Like Receptor 5 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Toll-Like Receptor 5 Antibody (YA3265) Product Image WB
  • Toll-Like Receptor 5 Antibody (YA3265) Product Image WB