Toll-Like Receptor 5 Antibody (YA3265)

Cat. No.: HY-P83520: Data Sheet COA
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Toll-Like Receptor 5 Antibody (YA3265) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Toll-Like Receptor 5.

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10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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Toll-Like Receptor 5 Antibody (YA3265) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
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Toll-Like Receptor 5 Antibody (YA3265) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Toll-Like Receptor 5.

Host

Rabbit

Clonality

Recombinant,Monoclonal

분자량

Predicted band size: 98 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O60602

Gene ID

7100 [NCBI]

Immunogen

A synthesized peptide derived from human TLR5 aa250-410.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), A549 (lane 2, 15 μg), HT-29 (lane 3, 15 μg), RAW264.7 (lane 4, 15 μg), and HaCaT (lane 5, 15 μg) using Toll-Like Receptor 5 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Western blot analysis of extracts from Hela (lane 2(40μg),SK-Br-3 (lane 3(40μg), A549 (lane 4(40μg),U-251 (lane 5(40μg)using Toll-Like Receptor 5 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

Function：Pattern recognition receptor (PRR) located on the cell surface that participates in the activation of innate immunity and inflammatory response (PubMed:11323673, PubMed:18490781). Recognizes small molecular motifs named pathogen-associated molecular pattern (PAMPs) expressed by pathogens and microbe-associated molecular patterns (MAMPs) usually expressed by resident microbiota (PubMed:29934223). Upon ligand binding such as bacterial flagellins, recruits intracellular adapter proteins MYD88 and TRIF leading to NF-kappa-B activation, cytokine secretion and induction of the inflammatory response (PubMed:11489966, PubMed:20855887). Plays thereby an important role in the relationship between the intestinal epithelium and enteric microbes and contributes to the gut microbiota composition throughout life (By similarity)

Subcellular Localization：Cell membrane; Single-pass type I membrane protein

Expression：
Tissue_specificity:Highly expressed on the basolateral surface of intestinal epithelia (PubMed:11489966) . Expressed also in other cells such as lung epithelial cells (PubMed:11489966, PubMed:18490781)

Subunit：Homodimer (PubMed:22173220). Interacts with MYD88 (via TIR domain) (PubMed:20855887). Interacts with TICAM1 (via TIR domain) (PubMed:20855887).

Database

Entrez Gene: 7100 Human ; 53791 Mouse ; 289337 Rat

SwissProt: O60602 Human ; Q9JLF7 Mouse ;

OMIM: 601744 Human

Research Field

Immunology

Synonyms

Toll-Like Receptor 5; SLEB1; TIL3; Tlr5; Toll like receptor 5 precursor

각종 서류

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Toll-Like Receptor 5 Antibody (YA3265) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Toll-Like Receptor 5 Antibody (YA3265)

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Toll-Like Receptor 5 Antibody (YA3265) Related Classifications

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