PRPF31 Antibody (YA3338)(PBS only)

Cat. No.: HY-P83593A: Data Sheet User Guide for Antibodies
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PRPF31 Antibody (YA3338) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PRPF31.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PRPF31 Antibody (YA3338) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PRPF31.

Host

Rabbit

Molecular Weight

Predicted band size: 55 kDa;

Observed band size: 55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q8WWY3

Gene ID

26121 [NCBI]

Immunogen

A synthetic peptide of human PRPF31 aa 380-393.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11867543, PubMed:20118938, PubMed:28781166). Required for the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome (PubMed:11867543)

Subcellular Localization：Nucleus; Nucleus speckle; Nucleus, Cajal body

Expression：
Tissue_specificity:Ubiquitously expressed

Isoforms & Post-Translational Modification：Q8WWY3 has 4 isomers: Q8WWY3-1: 55456 Da (predicted); Q8WWY3-2: 40779 Da (predicted); Q8WWY3-3: 30966 Da (predicted); Q8WWY3-4: 54909 Da (predicted).
Phosphorylated by PRP4K during spliceosome assembly

Subunit：Identified in the spliceosome B complex (PubMed:28781166). Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39 (PubMed:11867543, PubMed:16723661, PubMed:26912367).

Research Field

Epigenetics and Nuclear Signaling

Synonyms

Protein 61K; hPrp31; U4/U6 snRNP 61 kDa protein

Documentation

User Guide for Antibodies (1077 KB)