Caldesmon Antibody (YA4187)
(Synonyms: CDM; HCAD; LCAD; H-CAD; L-CAD; NAG22)Caldesmon Antibody (YA4187) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Caldesmon.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, IHC-P, FC, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
Caldesmon Antibody (YA4187) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Caldesmon.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 70-75 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 93 kDa
Purified recombinant fragment of human CALD1 (AA: 26-207) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS with 0.05% sodium azide
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Caldesmon is an Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration
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Subcellular Localization
Cytoplasm, cytoskeleton; Cytoplasm, myofibril; Cytoplasm, cytoskeleton, stress fiber
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Expression
Tissue_specificity:High molecular weight calmodulin (isoform 1) is primarily expressed in smooth muscle, while low molecular weight calmodulin (isoforms 2, 3, 4, and 5) are widely distributed in non-muscle tissues and cells. It is not expressed in skeletal muscle or the heart. -
Isoforms & Post-Translational Modification
Q05682 has 6 isomers: Q05682-1: 93231 Da (predicted); Q05682-2: 65707 Da (predicted); Q05682-3: 64256 Da (predicted); Q05682-4: 62663 Da (predicted); Q05682-5: 61213 Da (predicted); Q05682-6: 65620 Da (predicted).
In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization. CDK1-mediated phosphorylation promotes Schwann cell migration during peripheral nerve regeneration (By similarity) -
SwissProt ID
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Synonyms
CDM; HCAD; LCAD; H-CAD; L-CAD; NAG22
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