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CD127 Antibody (YA545)(PBS only)

(Synonyms: IL7R; Interleukin-7 receptor subunit alpha; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; CDw127; CD antigen CD127)

CD127 Antibody (YA545) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD127.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD127 Antibody (YA545)(PBS only)
  • CD127 Antibody (YA545)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

CD127 Antibody (YA545) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD127.

  • Host Rabbit
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 70 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 52 kDa
Immunogen

Synthetic peptide corresponding to Human CD127.The exact sequence is proprietary to MCE.

Purification

affinity purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD127 Antibody (YA545)(PBS only)
    Western blot analysis was performed on extracts from Daudi (lane 1, 20 μg), HeLa (lane 2, 20 μg), U2OS (lane 3, 20 μg), and K562 (lane 4, 20 μg) using CD127 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    CD127 (IL-7Rα, IL7R) is the IL-7 receptor α-chain and a key immune molecule for maintaining T-cell survival, development, and memory formation. It is highly expressed on naive and memory T cells but expressed at low levels on regulatory T cells (Tregs); therefore, it is widely used in T-cell subset identification, immune monitoring, and research into tumor immunology, infection immunology, and autoimmune diseases.

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Cell membrane; Single-pass type I membrane protein; Secreted

  • Isoforms & Post-Translational Modification

    CD127 has 4 isomers: P16871-1: 51579 Da (predicted); P16871-2: 34018 Da (predicted); P16871-3: 29936 Da (predicted); P16871-4: 28722 Da (predicted).
    N-glycosylated IL-7Ralpha binds IL7 300-fold more tightly than the unglycosylated form;Ubiquitinated by MARCHF8; leading to lysosomal degradation

  • Subunit

    The IL7 receptor is a heterodimer of IL7R and IL2RG. The TSLP receptor is a heterodimer of CRLF2 and IL7R. Interacts with CD53

  • SwissProt ID

    P16871

  • Gene ID
    3575 [NCBI]
  • Synonyms

    IL7R; Interleukin-7 receptor subunit alpha; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; CDw127; CD antigen CD127

  • Research Field

    Immunology

CD127 Antibody (YA545)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from Daudi (lane 1, 20 μg), HeLa (lane 2, 20 μg), U2OS (lane 3, 20 μg), and K562 (lane 4, 20 μg) using CD127 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • CD127 Antibody (YA545)(PBS only) Product Image
    1/16
  • Experimental Validation Results for CD127 Antibody (YA545)(PBS only)
  • CD127 Antibody (YA545)(PBS only) Product Image
  • Experimental Validation Results for CD127 Antibody (YA545)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from Daudi (lane 1, 20 μg), HeLa (lane 2, 20 μg), U2OS (lane 3, 20 μg), and K562 (lane 4, 20 μg) using CD127 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for CD127 Antibody (YA545)(PBS only)
  • CD127 Antibody (YA545)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from Daudi (lane 1, 20 μg), HeLa (lane 2, 20 μg), U2OS (lane 3, 20 μg), and K562 (lane 4, 20 μg) using CD127 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • CD127 Antibody (YA545)(PBS only) Product Image WB