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MOV10 Antibody (YA1669)

(Synonyms: gb110; MOV 10)
Customer Review

Based on 1 Customer Validation

MOV10 Antibody (YA1669) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MOV10.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for MOV10 Antibody (YA1669)
  • Experimental Validation Results for MOV10 Antibody (YA1669)
  • Experimental Validation Results for MOV10 Antibody (YA1669)
  • MOV10 Antibody (YA1669) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

MOV10 Antibody (YA1669) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MOV10.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 114 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 114 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Mov10 aa850-880.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104020

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MOV10 Antibody (YA1669)
    Western blot analysis of extracts from HEK293 (lane 2, 20ug), NIH/3T3 (lane 3, 20ug), RAW264.7 (lane 4, 20ug) and C6 (lane 5, 20ug) using MOV10 Antibody (HY-P81924). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MOV10 Antibody (YA1669)
    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using MOV10 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MOV10 Antibody (YA1669)
    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using MOV10 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    MOV10 is a 5' to 3' RNA helicase that is involved in a number of cellular roles ranging from mRNA metabolism and translation, modulation of viral infectivity, inhibition of retrotransposition, or regulation of synaptic transmission. Plays an important role in innate antiviral immunity by promoting type I interferon production. Mechanistically, specifically uses IKKepsilon/IKBKE as the mediator kinase for IRF3 activation. Blocks HIV-1 virus replication at a post-entry step. Counteracts HIV-1 Vif-mediated degradation of APOBEC3G through its helicase activity by interfering with the ubiquitin-proteasome pathway. Also inhibits hepatitis B virus/HBV replication by interacting with HBV RNA and thereby inhibiting the early step of viral reverse transcription. Contributes to UPF1 mRNA target degradation by translocation along 3' UTRs. Required for microRNA (miRNA)-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC. In cooperation with FMR1, regulates miRNA-mediated translational repression by AGO2. Restricts retrotransposition of long interspersed element-1 (LINE-1) in cooperation with TUT4 and TUT7 counteracting the RNA chaperonne activity of L1RE1. Facilitates LINE-1 uridylation by TUT4 and TUT7. Required for embryonic viability and for normal central nervous system development and function. Plays two critical roles in early brain development: suppresses retroelements in the nucleus by directly inhibiting cDNA synthesis, while regulates cytoskeletal mRNAs to influence neurite outgrowth in the cytosol. May function as a messenger ribonucleoprotein (mRNP) clearance factor; (Microbial infection) Required for RNA-directed transcription and replication of the human hepatitis delta virus (HDV). Interacts with small capped HDV RNAs derived from genomic hairpin structures that mark the initiation sites of RNA-dependent HDV RNA transcription[1][2][3][4][5][6][7][8][9][10][11][12][13].

  • Subcellular Localization

    Cytoplasm, P-body; Cytoplasm, Cytoplasmic ribonucleoprotein granule; Cytoplasm, Stress granule; Nucleus; Cytoplasm

  • Expression


    Induction:By herpes simplex 1/HHV-1 virus infection

  • Isoforms & Post-Translational Modification

    Q9HCE1 has 3 isomers: Q9HCE1-1: 113671 Da (predicted); Q9HCE1-2: 101598 Da (predicted); Q9HCE1-3: 6546 Da (predicted).
    Ubiquitinated by the DCX(DCAF12) complex that specifically recognizes the glutamate-leucine (Glu-Leu) degron at the C-terminus, leading to its degradation;(Microbial infection) Cleaved and targeted for degradation by picornavirus proteases

  • Subunit

    Interacts with DICER1, AGO2, TARBP2, EIF6 and RPL7A (60S ribosome subunit); they form a large RNA-induced silencing complex (RISC) (PubMed:17507929).

  • SwissProt ID

    Q9HCE1

  • Gene ID
    4343 [NCBI]
  • Synonyms

    gb110; MOV 10

  • Research Field

    Epigenetics and Nuclear Signaling

MOV10 Antibody (YA1669) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 2, 20ug), NIH/3T3 (lane 3, 20ug), RAW264.7 (lane 4, 20ug) and C6 (lane 5, 20ug) using MOV10 Antibody (HY-P81924). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using MOV10 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using MOV10 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • MOV10 Antibody (YA1669) Product Image
    1/16
  • Experimental Validation Results for MOV10 Antibody (YA1669)
  • Experimental Validation Results for MOV10 Antibody (YA1669)
  • Experimental Validation Results for MOV10 Antibody (YA1669)
  • MOV10 Antibody (YA1669) Product Image
  • Experimental Validation Results for MOV10 Antibody (YA1669)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 2, 20ug), NIH/3T3 (lane 3, 20ug), RAW264.7 (lane 4, 20ug) and C6 (lane 5, 20ug) using MOV10 Antibody (HY-P81924). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MOV10 Antibody (YA1669)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using MOV10 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MOV10 Antibody (YA1669)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using MOV10 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MOV10 Antibody (YA1669)
  • Experimental Validation Results for MOV10 Antibody (YA1669)
  • Experimental Validation Results for MOV10 Antibody (YA1669)
  • MOV10 Antibody (YA1669) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293 (lane 2, 20ug), NIH/3T3 (lane 3, 20ug), RAW264.7 (lane 4, 20ug) and C6 (lane 5, 20ug) using MOV10 Antibody (HY-P81924). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • MOV10 Antibody (YA1669) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using MOV10 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • MOV10 Antibody (YA1669) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using MOV10 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • MOV10 Antibody (YA1669) Product Image WB
  • MOV10 Antibody (YA1669) Product Image IHC-P
  • MOV10 Antibody (YA1669) Product Image IHC-P