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ETAA1 Antibody (YA2781)

(Synonyms: ETAA16)
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Based on 1 Customer Validation

ETAA1 Antibody (YA2781) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ETAA1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ETAA1 Antibody (YA2781)
  • ETAA1 Antibody (YA2781) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

ETAA1 Antibody (YA2781) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ETAA1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 95 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 103 kDa
Species Reactivity Database

Entrez Gene: 54465 Human

SwissProt: Q9NY74 Human

Immunogen

Recombinant protein of human ETAA1

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105024

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ETAA1 Antibody (YA2781)
    Western blot analysis of extracts from K562 (lane2, 20μg), MCF-7 (lane3, 20μg), Hela (lane4, 20μg) and HUVEC (lane5, 20μg) using ETAA1 Antibody (HY-P83036). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    ETAA1 is a Replication stress response protein that accumulates at DNA damage sites and promotes replication fork progression and integrity. Recruited to stalled replication forks via interaction with the RPA complex and directly stimulates ATR kinase activity independently of TOPBP1. Probably only regulates a subset of ATR targets[1][2][3][4].

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:Expressed at high levels in the brain, liver kidney and Ewing tumor cell lines

  • Subunit

    Interacts (via RBM1 motif) with RPA1 (PubMed:27601467, PubMed:27723717, PubMed:27723720). Interacts (via RBM2 motif) with RPA2 (PubMed:27601467, PubMed:27723717, PubMed:27723720).

  • SwissProt ID

    Q9NY74

  • Gene ID
    54465 [NCBI]
  • Synonyms

    ETAA16

  • Research Field

    Cancer

References

[1]. Feng S, et al. Ewing Tumor-associated Antigen 1 Interacts with Replication Protein A to Promote Restart of Stalled Replication Forks. J Biol Chem. 2016 Oct 14;291(42):21956-21962. [Content Brief]

[2]. Haahr P, et al. Activation of the ATR kinase by the RPA-binding protein ETAA1. Nat Cell Biol. 2016 Nov;18(11):1196-1207. [Content Brief]

[3]. Bass TE, et al. ETAA1 acts at stalled replication forks to maintain genome integrity. Nat Cell Biol. 2016 Nov;18(11):1185-1195. [Content Brief]

[4]. Saldivar JC, et al. An intrinsic S/G(2) checkpoint enforced by ATR. Science. 2018 Aug 24;361(6404):806-810. [Content Brief]

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ETAA1 Antibody (YA2781) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane2, 20μg), MCF-7 (lane3, 20μg), Hela (lane4, 20μg) and HUVEC (lane5, 20μg) using ETAA1 Antibody (HY-P83036). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • ETAA1 Antibody (YA2781) Product Image
    1/16
  • Experimental Validation Results for ETAA1 Antibody (YA2781)
  • ETAA1 Antibody (YA2781) Product Image
  • Experimental Validation Results for ETAA1 Antibody (YA2781)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane2, 20μg), MCF-7 (lane3, 20μg), Hela (lane4, 20μg) and HUVEC (lane5, 20μg) using ETAA1 Antibody (HY-P83036). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ETAA1 Antibody (YA2781)
  • ETAA1 Antibody (YA2781) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562 (lane2, 20μg), MCF-7 (lane3, 20μg), Hela (lane4, 20μg) and HUVEC (lane5, 20μg) using ETAA1 Antibody (HY-P83036). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • ETAA1 Antibody (YA2781) Product Image WB