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Digoxin Antibody (YA6545)

(Synonyms: 1672-46-4 antibody; BRN 0096479 antibody; DIG antibody; EINECS 216-806-2 antibody; HSDB 7108 antibody; Lanadigenin antibody; Lanadigigenin antibody; ST056392 antibody; Digoxigenin 地高辛)
Customer Review

Based on 1 Customer Validation

Digoxin Antibody (YA6545) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Digoxin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ELISA, IHC-P, IHC-F, Dot Blot, IF-Tissue, IP

  • Reactivity :

    Species independent

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Digoxin Antibody (YA6545)
  • Experimental Validation Results for Digoxin Antibody (YA6545)
  • Digoxin Antibody (YA6545) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
Dot Blot Info
 
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:2000 1:5000-1:20000 1:200-1:5000 1:50-1:1000 1:2000 1:50-1:1000 1-2μg/sample

Product Details

Description

Digoxin Antibody (YA6545) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Digoxin.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Species independent
Immunogen

DIG-OVA

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Digoxin Antibody (YA6545)
    Competitive ELISA analysis of Digoxin (HY-B1049) /Estradiol (HY-B0141)was performed by coating wells of a 96-well plate with 50 µl per well of Digoxin-BSA (HY-161533) diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with BSA blocking buffer, and incubated with 100 µl per well of Digoxin Antibody (HY-P86852) at concentration of 30 ng/mL with serial diluted Digoxin (HY-B1049) / Estradiol (HY-B0141) starting from a concentration of 1ug/ml for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated Goat Anti-Rabbit IgG H&L (HY-P8001) at a dilution of 1/1,0000 for one hour at room temperature. Detection was performed using TMB substrate for 8 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid, and absorbances were read on a spectrophotometer at 450 nm.
  • Experimental Validation Results for Digoxin Antibody (YA6545)
    Indirect ELISA analysis of Digoxin was performed by coating wells of a 96-well plate with 100 µl per well of digitoxin-BSA (HY-161533) at a concentration of 1 µg/mL overnight at 4 °C. Wells of the plate were washed and blocked with 1% BSA in 1× PBST buffer. The plate was then incubated with 100 µl per well of Digoxin Antibody (HY-P86852), starting at an initial concentration of 1μg/mL and followed by continuous dilution to 11 concentrations, for 2 hours at room temperature. The plate was washed and incubated with 100 µl per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody (HY-P8001) at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using TMB substrate for 8 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid, and absorbances were read on a spectrophotometer at 450 nm.

Background

  • Function

    Digoxin is a Digoxin is a potent inhibitor of Na+/K+-ATPase, has the potential for arrhythmia and heart failure research.

  • Synonyms

    1672-46-4 antibody; BRN 0096479 antibody; DIG antibody; EINECS 216-806-2 antibody; HSDB 7108 antibody; Lanadigenin antibody; Lanadigigenin antibody; ST056392 antibody; Digoxigenin 地高辛

Digoxin Antibody (YA6545) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Competitive ELISA analysis of Digoxin (HY-B1049) /Estradiol (HY-B0141)was performed by coating wells of a 96-well plate with 50 µl per well of Digoxin-BSA (HY-161533) diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with BSA blocking buffer, and incubated with 100 µl per well of Digoxin Antibody (HY-P86852) at concentration of 30 ng/mL with serial diluted Digoxin (HY-B1049) / Estradiol (HY-B0141) starting from a concentration of 1ug/ml for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated Goat Anti-Rabbit IgG H&L (HY-P8001) at a dilution of 1/1,0000 for one hour at room temperature. Detection was performed using TMB substrate for 8 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid, and absorbances were read on a spectrophotometer at 450 nm.

  • 1/16

    MedChemExpress Validation
     Indirect ELISA analysis of Digoxin was performed by coating wells of a 96-well plate with 100 µl per well of digitoxin-BSA (HY-161533) at a concentration of 1 µg/mL overnight at 4 °C. Wells of the plate were washed and blocked with 1% BSA in 1× PBST buffer. The plate was then incubated with 100 µl per well of Digoxin Antibody (HY-P86852), starting at an initial concentration of 1μg/mL and followed by continuous dilution to 11 concentrations, for 2 hours at room temperature. The plate was washed and incubated with 100 µl per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody (HY-P8001) at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using TMB substrate for 8 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid, and absorbances were read on a spectrophotometer at 450 nm.

  • Digoxin Antibody (YA6545) Product Image
    1/16
  • Experimental Validation Results for Digoxin Antibody (YA6545)
  • Experimental Validation Results for Digoxin Antibody (YA6545)
  • Digoxin Antibody (YA6545) Product Image
  • Experimental Validation Results for Digoxin Antibody (YA6545)
    1/1
    MedChemExpress Validation
     Competitive ELISA analysis of Digoxin (HY-B1049) /Estradiol (HY-B0141)was performed by coating wells of a 96-well plate with 50 µl per well of Digoxin-BSA (HY-161533) diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with BSA blocking buffer, and incubated with 100 µl per well of Digoxin Antibody (HY-P86852) at concentration of 30 ng/mL with serial diluted Digoxin (HY-B1049) / Estradiol (HY-B0141) starting from a concentration of 1ug/ml for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated Goat Anti-Rabbit IgG H&L (HY-P8001) at a dilution of 1/1,0000 for one hour at room temperature. Detection was performed using TMB substrate for 8 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid, and absorbances were read on a spectrophotometer at 450 nm.
  • Experimental Validation Results for Digoxin Antibody (YA6545)
    1/1
    MedChemExpress Validation
     Indirect ELISA analysis of Digoxin was performed by coating wells of a 96-well plate with 100 µl per well of digitoxin-BSA (HY-161533) at a concentration of 1 µg/mL overnight at 4 °C. Wells of the plate were washed and blocked with 1% BSA in 1× PBST buffer. The plate was then incubated with 100 µl per well of Digoxin Antibody (HY-P86852), starting at an initial concentration of 1μg/mL and followed by continuous dilution to 11 concentrations, for 2 hours at room temperature. The plate was washed and incubated with 100 µl per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody (HY-P8001) at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using TMB substrate for 8 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid, and absorbances were read on a spectrophotometer at 450 nm.
  • Experimental Validation Results for Digoxin Antibody (YA6545)
  • Experimental Validation Results for Digoxin Antibody (YA6545)
  • Digoxin Antibody (YA6545) Product Image
    MedChemExpress Validation

    Competitive ELISA analysis of Digoxin (HY-B1049) /Estradiol (HY-B0141)was performed by coating wells of a 96-well plate with 50 µl per well of Digoxin-BSA (HY-161533) diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with BSA blocking buffer, and incubated with 100 µl per well of Digoxin Antibody (HY-P86852) at concentration of 30 ng/mL with serial diluted Digoxin (HY-B1049) / Estradiol (HY-B0141) starting from a concentration of 1ug/ml for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated Goat Anti-Rabbit IgG H&L (HY-P8001) at a dilution of 1/1,0000 for one hour at room temperature. Detection was performed using TMB substrate for 8 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid, and absorbances were read on a spectrophotometer at 450 nm.

  • Digoxin Antibody (YA6545) Product Image
    MedChemExpress Validation

    Indirect ELISA analysis of Digoxin was performed by coating wells of a 96-well plate with 100 µl per well of digitoxin-BSA (HY-161533) at a concentration of 1 µg/mL overnight at 4 °C. Wells of the plate were washed and blocked with 1% BSA in 1× PBST buffer. The plate was then incubated with 100 µl per well of Digoxin Antibody (HY-P86852), starting at an initial concentration of 1μg/mL and followed by continuous dilution to 11 concentrations, for 2 hours at room temperature. The plate was washed and incubated with 100 µl per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody (HY-P8001) at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using TMB substrate for 8 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid, and absorbances were read on a spectrophotometer at 450 nm.

  • Digoxin Antibody (YA6545) Product Image ELISA
  • Digoxin Antibody (YA6545) Product Image ELISA