CDK8 Antibody (YA1323)
(Synonyms: Cyclin-dependent kinase 8)
Based on 1 Customer Validation
CDK8 Antibody (YA1323) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDK8.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IHC-F, IF-Tissue
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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|---|---|---|---|---|
| Dilution Ratio | 1:500-1000 | 1:100-500 | 1:100-500 | 1:100-500 |
Product Details
CDK8 Antibody (YA1323) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDK8.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size:55 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 53 kDa
Entrez Gene: 1024 Human ; 264064 Mouse ;
SwissProt: P49336 Human ; Q8R3L8 Mouse ;
OMIM: 618748 Human
KLH conjugated synthetic peptide derived from human CDK8.
Endogenous
Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CDK8 (cyclin-dependent kinase 8) is a transcription-associated kinase that functions within the Mediator kinase module and regulates RNA polymerase II-dependent gene expression through reversible association with the Mediator complex.[1][2] Mechanistically, CDK8 integrates developmental, metabolic, inflammatory, and signal-responsive transcriptional programs by modulating transcription factor activity and enhancer-driven transcriptional output.[2][3] In disease-relevant contexts, dysregulation of CDK8 signaling has been linked to multiple human disorders, particularly cancer, where Mediator kinase activity contributes to transcriptional reprogramming and tumor-promoting gene expression networks.[2][4] Experimental studies further demonstrate that CDK8 and CDK19 regulate intestinal lineage specification through Mediator-dependent control of chromatin-remodeling mechanisms and enhancer function.[5] Compared with the related isoform CDK19, CDK8 is more broadly expressed across tissues, whereas CDK19 shows greater tissue specificity; however, both kinases display highly overlapping transcriptional and phosphoregulatory functions and often act redundantly within the Mediator kinase module.[3] Therefore, genetic disruption of a single paralog frequently produces partial phenotypes, while dual inhibition more closely recapitulates Mediator kinase suppression.[3] For experimental applications, most currently reported Mediator kinase inhibitors target both CDK8 and CDK19, and these compounds are widely used to investigate transcriptional reprogramming, signal-induced gene expression, and therapeutic vulnerabilities in cancer models.[3][4]
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Subcellular Localization
Nucleus
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Isoforms & Post-Translational Modification
P49336 has 2 isomers: P49336-1: 53284 Da (predicted); P49336-2: 53156 Da (predicted).
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Subunit
Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. The cylin/CDK pair formed by CCNC/CDK8 also associates with the large subunit of RNA polymerase II. Interacts with CTNNB1, GLI3 and MAML1
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SwissProt ID
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Synonyms
Cyclin-dependent kinase 8
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Research Field
Cell biology
Documentation
[1]. Fant CB, et al. Regulatory functions of the Mediator kinases CDK8 and CDK19. Transcription. 2019 Apr;10(2):76-90. [Content Brief]
[2]. Dannappel MV, et al. Molecular and in vivo Functions of the CDK8 and CDK19 Kinase Modules. Front Cell Dev Biol. 2019 Jan 14;6:171. doi: 10.3389/fcell.2018.00171. PMID: 30693281; PMCID: PMC6340071. [Content Brief]
[3]. Chen M, et al. CDK8 and CDK19: positive regulators of signal-induced transcription and negative regulators of Mediator complex proteins. Nucleic Acids Res. 2023 Aug 11;51(14):7288-7313. doi: 10.1093/nar/gkad538. PMID: 37378433; PMCID: PMC10415139. [Content Brief]
[4]. Luyties O, et al. The Mediator kinase module: an interface between cell signaling and transcription. Trends Biochem Sci. 2022 Apr;47(4):314-327. doi: 10.1016/j.tibs.2022.01.002. Epub 2022 Feb 19. PMID: 35193797; PMCID: PMC8939868. [Content Brief]
[5]. Dannappel MV, et al. CDK8 and CDK19 regulate intestinal differentiation and homeostasis via the chromatin remodeling complex SWI/SNF. J Clin Invest. 2022 Oct 17;132(20):e158593. doi: 10.1172/JCI158593. PMID: 36006697; PMCID: PMC9566890. [Content Brief]
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