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KIF15 Antibody (YA8915)

(Synonyms: 230049F1, hKLP2, Kinesin-like protein 2, Kinesin-like protein 7, Kinesin-like protein KIF15)
Customer Review

Based on 1 Customer Validation

KIF15 Antibody (YA8915) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to KIF15.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IP, ELISA

  • Reactivity :

    human

  • Formulation:

    Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for KIF15 Antibody (YA8915)
  • KIF15 Antibody (YA8915) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1000 1-2μg per 100-500μg Total protein 1:50-3000

Product Details

Description

KIF15 Antibody (YA8915) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to KIF15.

  • Host Mouse
  • Species Reactivity
    human
  • Calculated Molecular Weight Predicted band size: 160 kDa
Immunogen

A synthesized peptide derived from human KIF15.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at 2-8°C for 1 year, do not freeze.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for KIF15 Antibody (YA8915)
    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), HepG2 (lane 3), K562 (lane 4), THP-1 (lane 5), and SH-SY5Y (lane 6) using KIF15 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    KIF15 is a Plus-end directed kinesin-like motor enzyme involved in mitotic spindle assembly

  • Subcellular Localization

    Cytoplasm; Cytoplasm, cytoskeleton, spindle

  • Expression


    Tissue_Specificity: Expressed in testis, colon, thymus and in breast cancer.

  • Isoforms & Post-Translational Modification

    KIF15 has 4 isoforms, Q9NS87-1: amino acid length is 1388, molecular weight is 160160 Da (predicted); Q9NS87-2: amino acid length is 1291, molecular weight is 149784 Da (predicted); Q9NS87-3: amino acid length is 436, molecular weight is 51433 Da (predicted); Q9NS87-4: amino acid length is 1199, molecular weight is 137169 Da (predicted).

  • Subunit

    Interacts with MKI67 and TPX2

  • SwissProt ID

    Q9NS87

  • Gene ID
    56992 [NCBI]
  • Synonyms

    230049F1, hKLP2, Kinesin-like protein 2, Kinesin-like protein 7, Kinesin-like protein KIF15

KIF15 Antibody (YA8915) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), HepG2 (lane 3), K562 (lane 4), THP-1 (lane 5), and SH-SY5Y (lane 6) using KIF15 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • KIF15 Antibody (YA8915) Product Image
    1/16
  • Experimental Validation Results for KIF15 Antibody (YA8915)
  • KIF15 Antibody (YA8915) Product Image
  • Experimental Validation Results for KIF15 Antibody (YA8915)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), HepG2 (lane 3), K562 (lane 4), THP-1 (lane 5), and SH-SY5Y (lane 6) using KIF15 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for KIF15 Antibody (YA8915)
  • KIF15 Antibody (YA8915) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), HepG2 (lane 3), K562 (lane 4), THP-1 (lane 5), and SH-SY5Y (lane 6) using KIF15 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • KIF15 Antibody (YA8915) Product Image WB